Detection of hepatitis B virus core mutants by PCR-RFLP in chronically infected patients

Barros, M.F.; Rodrigues, Pierre; Matias, S.R; Carreira, L. Miguel; Machado-Caetano, J. A.

doi:10.1016/j.jviromet.2004.03.009

Cited by 4 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HBeAg and HBcAg, which share a large number of amino acids (AA) and some epitopes for B-and T-cells, are important targets for antiviral immunity [7][8][9]. HBcAg, which is more immunogenic than hepatitis B surface antigen (HBsAg) and HBeAg proteins, is a major target for immune-mediated viral clearance, inducing B-cell, T-helper and cytotoxic T-lymphocyte responses.…”

mentioning

confidence: 99%

See 1 more Smart Citation

HBV Core Region Variability: Effect of Antiviral Treatments on Main Epitopic Regions

et al. 2011

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 99%

“…In both cases, the highest Av12 value corresponded to mutant cases. Patients with changes in the B74-84 region showed higher HBV DNA levels (mean [range] 6.9 [4-12] log 10 copies/ml) than those without alterations in this region (mean [range] 5.8[4][5][6][7][8][9] log 10 copies/ml; P=0.04).…”

mentioning

confidence: 99%

HBV Core Region Variability: Effect of Antiviral Treatments on Main Epitopic Regions

et al. 2011

View full text Add to dashboard Cite

show abstract

“…In the late 1990s, RFLP was demonstrated to sensitively detect wild‐type and mutant HBV strains at codons 204, 528 and 552 in a mixed viral population . Although RFLP has also been applied to detect core mutants , it is commonly used to detect variants in the YMDD motif . The major disadvantage is the requirement for combinations of different restriction enzymes that must be carefully designed for each mutant of interest.…”

Section: Standard Approaches To Describe Genomic Variabilitymentioning

confidence: 99%

Current molecular methods for the detection of hepatitis B virus quasispecies

Rybicka

Stalke

Bielawski

2016

Reviews in Medical Virology

View full text Add to dashboard Cite

Chronic HBV infection affects more than 240 million people worldwide and is associated with a broad range of clinical manifestations including liver cirrhosis, liver failure and hepatocellular carcinoma. Because of the lack of an efficient cure for chronic hepatitis B, the main goal of antiviral therapy is the prevention of liver disease progression coupled with prolonged survival of patients. Because HBV viral load has been shown to be a crucial determinant of the progression of liver damage, these goals can be achieved as long as HBV replication can be suppressed. Unfortunately, long-term therapy with the low-to-moderate genetic barrier drugs, which are still recommended in a majority of developing countries, are strongly associated with HBV resistance development and treatment failure. In such cases, the precise and accurate determination of drug-resistant variants in an individual patient before treatment is important for a proper choice of first-line potent therapy. Nowadays, a number of techniques are available to study HBV quasispecies evolution. This review describes the advantages and limitations of various assays detecting drug-resistant HBV variants. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

Identification of hepatitis B virus core mutants by PCR-RFLP in chronic hepatitis B patients from Punjab, Pakistan

et al. 2007

View full text Add to dashboard Cite

Chronic hepatitis B virus (HBV) infection remains a major health issue worldwide. Several factors including core gene variation are responsible for the development of chronicity of HBV infection. The present study was designed to identify the variations in the core region of the HBV genome in a local population of chronic hepatitis B patients (n = 57) using a PCR-based restriction fragment length polymorphism (PCR-RFLP) method. Fifty subjects were found to be positive for the presence of HBV DNA. For the core region genotyping, the Ava II and Msp I restriction enzymes were used. Mutations at nucleotide (nt) 2147 and nt 2362 in the HBV genome in the core region for Ava II (A4 type, 74%) and nt 2331 for Msp I (M1 type, 66%) were observed as the most common pattern. These results are different from those of previously reported studies on other populations and thus appear to be unique to the Pakistani population. This type of characterization of core mutants may be useful for the design of vaccines based on viral epitopes that are effective for the Pakistani population. Moreover, these unique genotypic patterns for the HBV core gene might be some of the main factors responsible for understanding the underlying mechanism by which HBV chronicity is developed in the Pakistani population.

show abstract

Detection of hepatitis B virus core mutants by PCR-RFLP in chronically infected patients

Cited by 4 publications

References 12 publications

HBV Core Region Variability: Effect of Antiviral Treatments on Main Epitopic Regions

HBV Core Region Variability: Effect of Antiviral Treatments on Main Epitopic Regions

Current molecular methods for the detection of hepatitis B virus quasispecies

Identification of hepatitis B virus core mutants by PCR-RFLP in chronic hepatitis B patients from Punjab, Pakistan

Contact Info

Product

Resources

About