Detection of hepatitis B virus DNA in paraffin-embedded intrahepatic and extrahepatic cholangiocarcinoma tissue in the northern Chinese population

Wu, Yiqi; Wang, Tianzhen; Ye, Shengqian; Zhao, Ruihua; Bai, Xiaohui; Wu, Yingying; Abe, Kenji; Jin, Xiaoming

doi:10.1016/j.humpath.2011.04.004

Cited by 14 publications

(13 citation statements)

References 29 publications

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“…Sample collection was approved by the Harbin Medical University (Harbin, China) Institutional Ethics Committee. Formalin-fixed paraffin-embedded sections were incubated with t-Src and p-Y416Src antibodies, as described previously [19]. Briefly, the sections were heated for antigen retrieval at 95°C and blocked with 10% goat serum for 1 h. The slides were stained using the standard indirect horseradish peroxidase method, as described above for immunocytochemistry.…”

Section: Methodsmentioning

confidence: 99%

EF24 Suppresses Invasion and Migration of Hepatocellular Carcinoma CellsIn Vitrovia Inhibiting the Phosphorylation of Src

Zhao

Tin

Zhang

et al. 2016

BioMed Research International

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Diphenyl difluoroketone (EF24), a curcumin analog, is a promising anticancer compound that exerts its effects by inhibiting cell proliferation and inducing apoptosis. However, the efficacy of EF24 against cancer metastasis, particularly in hepatocellular carcinoma (HCC), remains elusive. In this study, the effect of EF24 on HCCLM-3 and HepG2 cell migration and invasion was detected by wound healing and transwell assay, respectively. The results revealed that EF24 suppressed the migration and invasion of both HCCLM-3 and HepG2 cells. Furthermore, EF24 treatment decreased the formation of filopodia on the cell surface and inhibited the phosphorylation of Src in both cell lines, which may help contribute towards understanding the mechanism underlying the suppressive effect of EF24 on HCC migration and invasion. Additionally, the expression of total- and phosphorylated-Src in primary HCC tissues and their paired lymph node metastatic tissues was detected, and phosphorylated-Src was found to be associated with HCC lymph node metastasis. The results of this study suggest that Src is a novel and promising therapeutic target in HCC and provide evidence to support the hypothesis that EF24 may be a useful therapeutic agent for the treatment of HCC.

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Section: Methodsmentioning

confidence: 99%

EF24 Suppresses Invasion and Migration of Hepatocellular Carcinoma CellsIn Vitrovia Inhibiting the Phosphorylation of Src

Zhao

Tin

Zhang

et al. 2016

BioMed Research International

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“…In a study from the United States, HBV and/or HCV DNA was present in 3 (27%) out of 11 ICC tissue samples obtained at the time of surgical resection [24]. Another study from China also showed that HBV DNA was detected in 34.8% (8/23) of ICC cases [25]. Recently, Torbenson et al [26] found that dysplasia of the intrahepatic bile ducts, the histologic precursor lesion of ICC, was present in HCV related cirrhotic livers.…”

Section: Discussionmentioning

confidence: 99%

Hepatitis viruses infection and risk of intrahepatic cholangiocarcinoma: evidence from a meta-analysis

Zhou

Zhao

et al. 2012

BMC Cancer

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BackgroundStudies investigating the association between Hepatitis B virus (HBV) and hepatitis C virus (HCV) infections and intrahepatic cholangiocarcinoma (ICC) have reported inconsistent findings. We conducted a meta-analysis of epidemiological studies to explore this relationship.MethodsA comprehensive search was conducted to identify the eligible studies of hepatitis infections and ICC risk up to September 2011. Summary odds ratios (OR) with their 95% confidence intervals (95% CI) were calculated with random-effects models using Review Manager version 5.0.ResultsThirteen case–control studies and 3 cohort studies were included in the final analysis. The combined risk estimate of all studies showed statistically significant increased risk of ICC incidence with HBV and HCV infection (OR = 3.17, 95% CI, 1.88-5.34, and OR = 3.42, 95% CI, 1.96-5.99, respectively). For case–control studies alone, the combined OR of infection with HBV and HCV were 2.86 (95% CI, 1.60-5.11) and 3.63 (95% CI, 1.86-7.05), respectively, and for cohort studies alone, the OR of HBV and HCV infection were 5.39 (95% CI, 2.34-12.44) and 2.60 (95% CI, 1.36-4.97), respectively.ConclusionsThis study suggests that both HBV and HCV infection are associated with an increased risk of ICC.

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“…HBV infection has also been associated with cancers at other extrahepatic sites (e.g., colorectum, kidney and ovaries) . Besides the hepatocytes, HBV DNA sequences have been found in extrahepatic tissues such as pancreas, biliary tract and kidney, as well as lymphatic tissue and peripheral mononuclear cells, thus making associations with cancer biologically plausible …”

Section: Introductionmentioning

confidence: 99%

Hepatitis B virus infection and the risk of cancer in the elderly US population

Mahale

Engels

Koshiol

2018

Intl Journal of Cancer

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Hepatitis B virus (HBV) infection causes hepatocellular carcinoma (HCC). Associations with other cancers are not established. We systematically assessed associations between HBV infection and cancers in the US elderly population. We conducted a case-control study using the Surveillance, Epidemiology, and End Results (SEER)-Medicare database in US adults aged ≥66 years. Cases (N = 1,825,316) were people with first cancers diagnosed in SEER registries (1993-2013). Controls (N = 200,000) were randomly selected, cancer-free individuals who were frequency-matched to cases on age, sex, race and calendar year. Associations with HBV infection (ascertained by Medicare claims) were assessed by logistic regression. HBV prevalence was higher in cases than controls (0.6% vs. 0.5%). HBV was positively associated with cancers of the stomach (adjusted odds ratio [aOR] = 1.19; 95% confidence intervals [CI] = 1.03-1.37), anus (1.66; 1.17-2.33), liver (10.6; 9.66-11.6), intrahepatic bile ducts (1.67; 1.18-2.37), nasopharynx (2.08; 1.33-3.25), as well as myelodysplastic syndrome (1.26; 1.07-1.49) and diffuse large B-cell lymphoma (DLBCL) (1.24; 1.06-1.46). Inverse associations were observed with female breast (aOR = 0.86; 95%CI = 0.76-0.98) and prostate (0.81; 0.73-0.91) cancers and chronic lymphocytic leukemia (0.77; 0.62-0.96). Associations were maintained in sensitivity analyses conducted in people without claims for cirrhosis or hepatitis C or human immunodeficiency virus infections. HBV infection is associated with increased risk of cancers other than HCC, such as bile duct cancers and DLBCL. The biological mechanisms by which HBV may lead to these cancers need to be explored.

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Detection of hepatitis B virus DNA in paraffin-embedded intrahepatic and extrahepatic cholangiocarcinoma tissue in the northern Chinese population

Cited by 14 publications

References 29 publications

EF24 Suppresses Invasion and Migration of Hepatocellular Carcinoma CellsIn Vitrovia Inhibiting the Phosphorylation of Src

EF24 Suppresses Invasion and Migration of Hepatocellular Carcinoma CellsIn Vitrovia Inhibiting the Phosphorylation of Src

Hepatitis viruses infection and risk of intrahepatic cholangiocarcinoma: evidence from a meta-analysis

Hepatitis B virus infection and the risk of cancer in the elderly US population

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