Detection of hepatitis B virus in the cerumen of patients with chronic hepatitis B infection

Eftekharian, Ali; Moghaddasi, Hamid; Gachkar, Latif; Amlashi, S S A

doi:10.1017/s0022215113002314

Cited by 5 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, the presence of DCH DNA in body fluid specimens other than blood in this case might indicate the hematogenous spreading of DCH DNA. This result agrees with the evidence in humans showing that HBV viral DNA can be independently present in cerumen specimens without any blood contamination, as they ruled out the presence of blood in the collected specimens using a specific Meyer test ( 21 ).…”

Section: Discussionsupporting

confidence: 91%

“…Statistically, this study revealed that DCH viremia cats with DCH DNA levels in the blood higher than 7 LC/mL are 3.5 and 4 times more likely to express DCH DNA in OS and RS specimens, respectively. The result in this study raised supporting evidence that mirrors the characteristic of HBV, where a correlation between the presence of high levels of HBV DNA in the serum with the presence of HBV DNA in other body fluids such as cerumen, saliva, nasopharyngeal swab, tears, and sweat has been documented in chronically infected humans ( 12 , 21 , 24 – 26 ). The increasing level of HBV DNA in the blood of HBV chronically infected patients is commonly seen during the HBV reactivation period due to various underlying causes that promotes immunosuppressive conditions, such as prior human immunodeficiency virus infection, chronic diseases, therapeutic intervention and/or chemotherapy, and inadequate host immune response ( 27 ).…”

Section: Discussionsupporting

confidence: 77%

“…This concern was especially highlighted in settings where close contact with chronically infected patients that showed no apparent clinical signs was inevitable, such in children's daycares or preliminary schools ( 12 , 13 ). Correspondingly, the detection of HBV DNA in various body fluid specimens other than blood, including cerumen, sweat, nasopharyngeal swab, urine, saliva, and tears, has been reported in numerous studies ( 3 , 12 , 14 , 15 , 20 , 21 ). In contrast to the broad evidence demonstrating the detection of HBV DNA in various body fluid specimens from infected patients, to date, there has been no report on the detection of DCH DNA in body fluid specimens other than blood in cats, although such an investigation was attempted in an earlier study ( 16 ).…”

Section: Discussionmentioning

confidence: 92%

See 2 more Smart Citations

Quantification of domestic cat hepadnavirus DNA in various body fluid specimens of cats: the potential viral shedding routes

Wardhani,

Tummaruk,

Piewbang

et al. 2023

Front. Vet. Sci.

View full text Add to dashboard Cite

Domestic cat hepadnavirus (DCH) belongs to the Hepadnaviridae family together with human hepatitis B virus (HBV) that remains to be a major health problem worldwide. The transmission of HBV infectious virion has been one of the essential factors that contribute to high number of HBV infection in humans. It has been long known that various body fluid specimens of human with chronic HBV infection contain HBV DNA and demonstrated to be infectious. In contrast to this knowledge, the detection of DCH in various body fluid specimens of cats, has not been reported. This study explored the detection of DCH DNA in various body fluid specimens of cats by quantitative polymerase chain reaction (qPCR) and investigated whether the detection of DCH DNA from broader routes was correlated with any genomic diversity by phylogenetic analysis. A total of 1,209 body fluid specimens were included, and DCH DNA was detected not only in 4.70% (25/532) of blood samples; but also in 12.5% (1/8), 1.14% (1/88), 2.54% (10/394), and 1.65% (3/182) of auricular swab (AS), nasal swab (NS), oral swab (OS), and rectal swab (RS) specimens, respectively. Furthermore, the level of DCH DNA detected in the blood was significantly correlated with DCH DNA detection in OS (P = 0.02) and RS (P = 0.04) specimens. Genomic analysis revealed that there was no notable genomic diversity within the complete genome sequences obtained in this study. In conclusion, this study highlighted the presence of DCH DNA in various body fluid specimens of cats, and the potential role of these specimens in DCH horizontal transmission within the cat population warrants further studies.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 92%

See 1 more Smart Citation

Quantification of domestic cat hepadnavirus DNA in various body fluid specimens of cats: the potential viral shedding routes

Wardhani,

Tummaruk,

Piewbang

et al. 2023

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“… Recientemente, el hallazgo de que el cerumen es capaz de transmitir el virus de la hepatitis B obliga a tomar precauciones apropiadas para evitar su transmisión 30 .…”

Section: Procedimiento De Irrigaciónunclassified

Extracción de la cera de los oídos

Orejas

Redondo²,

Vicente³

et al. 2015

Rev Pediatr Aten Primaria

View full text Add to dashboard Cite

Extraction of wax from the earA través de cuatro artículos sucesivos pretendemos mostrar los procedimientos que consideramos de mayor utilidad para el diagnóstico y el seguimiento de la otitis media serosa (OMS) por parte de Pediatría de Atención Primaria. En este primero, expondremos la que, a nuestro juicio, es la manera más eficaz de limpiar la cera del oído de un niño. Las conclusiones aportadas conjugan las recomendaciones ofrecidas por las principales guías sobre extracción de cera del oído y las de la revisión bibliográfica efectuada, con la experiencia de un equipo de pediatras y de otorrinolaringólogos de la misma área de salud. Alrededor de un 50% de niños requieren limpiar de cera sus oídos a fin de realizar una correcta otoscopia. Para ello podemos utilizar la instilación de cerumenolíticos, la irrigación, la extracción manual o cualquier combinación. No existe evidencia en la bibliografía de que un procedimiento sea mejor que otro. Conclusiones: tras aplicar las diferentes técnicas de limpieza, consideramos que, si la cera es externa y el conducto auditivo permeable, la mejor manera de eliminarla es mediante el uso de curetas o portaalgodones, pero si la cera es más profunda o está impactada, el método elegido será el lavado con irrigación de agua templada, para lo que un cerumenolítico aplicado previamente es de gran ayuda. Aconsejamos realizar dicha irrigación con una jeringa de 20 cc y un catéter intravenoso Abocat ® del 14-16, a fin de evitar riesgos.Through four successive articles we aim to show the procedures we consider to be most useful for the diagnosis and follow-up of otitis media with effusion (OME) by Primary care Pediatrics. In the first one, we expose what we believe is the best way to clean wax from the ear of a child. The conclusions provided combine the recommendations offered by the main guides on extraction of wax from the ear and the literature review carried out, with the experience of a team of pediatricians and otolaryngologists from the same Healthcare Area. Around 50% of children require clean wax from their ears in order to perform a correct otoscopy. To do this we can use the instillation of cerumenolytics, irrigation, manual removal, or any combination. There is no evidence in the literature that a procedure is better than another one. Conclusions: after applying different cleaning techniques, we believe that if the wax is external and the ear canal permeable, the best way to remove it is by the use of blunt ear curettes or applicator with triangular tip, but if the wax is deeper or is impacted, the better choice will be washing with warm water irrigation, in this case, the previous application of a cerumenolytic will be of great help. We advise to perform such irrigation with a syringe of 20 cc and an intravenous catheter Abocat ® 14-16, in order to avoid risks.

show abstract

“…They can, however, potentially transmit infection. Eftekharian and colleagues 3 demonstrate that hepatitis B virus DNA can be routinely isolated from the cerumen of hepatitis B infected patients. Thus, cerumen can be a potential source of transmission of hepatitis B virus.…”

mentioning

confidence: 99%

Focus on the external auditory canal

Youngs¹,

Fisher²

2013

J. Laryngol. Otol.

View full text Add to dashboard Cite

Detection of hepatitis B virus in the cerumen of patients with chronic hepatitis B infection

Abstract: Cerumen can be a potential source of transmission of hepatitis B virus.

Cited by 5 publications

References 13 publications

Quantification of domestic cat hepadnavirus DNA in various body fluid specimens of cats: the potential viral shedding routes

Quantification of domestic cat hepadnavirus DNA in various body fluid specimens of cats: the potential viral shedding routes

Extracción de la cera de los oídos

Focus on the external auditory canal

Contact Info

Product

Resources

About