Detection of hepatitis C virus by polym erase chain reaction in haemodialysed patients in relationship to anti-HCV status

Bouchardeau, Françoise; Chauveau, P; Marrec, N. Le; Girault, A; Zins, B.; Couroucé, Anne‐Marie

doi:10.1016/s0923-2516(06)80034-8

Cited by 13 publications

(10 citation statements)

References 27 publications

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“…Hemodialysis may be beneficial for the liver disease in patients infected with HCV with end-stage renal diseases as it induces a decrease in viremia levels and stimulates HGF production [Bouchardeau et al, 1993;Chan et al, 1993;Rampino et al, 1998Rampino et al, , 1999. However, whether the hemodialysis schedule and type of hemodialysis membrane used affect both the HCV RNA and HGF levels has not been investigated.…”

Section: Discussionmentioning

confidence: 99%

“…Hemodialysis patients with chronic HCV infection usually have normal or near normal aminotransferase levels [Bouchardeau et al, 1993;Chan et al, 1993] and their serum HCV RNA concentrations are lower than those found in patients with chronic hepatitis C without renal disease [Umlanft et al, 1997]. Furthermore, liver biopsy findings in hemodialysis patients with chronic HCV infection suggest that liver damage is milder than that in nonuremic patients [Akpolat et al, 1998;Rampino et al, 1999].…”

Section: Introductionmentioning

confidence: 99%

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Effect of hemodialysis schedules and membranes on hepatocyte growth factor and hepatitis C virus RNA levels

Barril¹,

Bartolomé²,

Sanz³

et al. 2010

Journal of Medical Virology

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Hemodialysis induces production of the hepatocyte growth factor (HGF) and decrease of serum hepatitis C virus (HCV) RNA in patients with HCV infection, but it is not known if the hemodialysis schedule or type of membrane affect both the HGF production and HCV viremia. The effects on both parameters of alternate-day intermittent hemodialysis and short-daily hemodialysis and high and low flux membranes were investigated in 41 patients treated by hemodialysis. Sixteen (39%) patients were anti-HCV positive and 11 (69%) had HCV RNA. Twenty-six patients were on alternate-day intermittent and 15 on short-daily hemodialysis. High flux membranes were used for 29 patients and low flux membranes for 12 patients. A decrease in HCV RNA was observed at the end of hemodialysis (8.6 x 10(5) +/- 1.1 x 10(6) IU/ml vs. 4.4 x 10(5) +/- 7.3 x 10(5) IU/ml, P = 0.003). The proportion of HCV RNA decrease was similar in patients dialyzed with both schedules and with both types of membranes. The HGF levels increased from 2,605.9 +/- 1,428.7 to >8,000 pg/ml at 15 min. At the end of the session, the HGF levels decreased to 5,106.7 +/- 2,533.9 pg/ml. The HGF levels at the start of the next session were similar to those at baseline (2,680.0 +/- 1,209.3 pg/ml). The increase and dynamics of the HGF levels were similar in patient's hemodialyzed with both schedules and with both types of membranes. These results suggest that changes in HCV RNA and HGF levels during hemodialysis are not influenced by the schedule or type of membrane used.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of hemodialysis schedules and membranes on hepatocyte growth factor and hepatitis C virus RNA levels

Barril¹,

Bartolomé²,

Sanz³

et al. 2010

Journal of Medical Virology

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“…The majority of HDpatients with HCVhave only a minimal derangement in the ALTlevel (1,6). However, recent histological analysis of the liver tissue indicates that HDpatients with HCVoften have chronic active hepatitis or cirrhosis (7,8).…”

Section: Discussionmentioning

confidence: 99%

“…The levels of serum aminotransferases are usually normal or only slightly elevated in HDpatients with HCV (1,6). Uremia is considered to predispose a patient to a chronic viral carrier status with little inflammatory activity in the liver.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Characterization of Hepatitis C Virus in Hemodialysis Patients.

et al. 1999

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Object Chronic hepatitis C virus (HCV) infection is commonin hemodialysis (HD) patients. In the present study, the prevalence and properties of HCVin HDpatients were analyzed. Methods and Results Of 125 HDpatients, 34 (27%) were positive for antibody to HCV,and HCV-RNA was detected in 23 (68%) of the 34 patients using reverse transcription polymerase chain reaction. The HCV-RNA sequence analysis did not identify the alterations specific to HDpatients with HCV,although one patient had a variant virus containing the deletion of the core gene sequence. Whenserial changes in the levels of HCV-RNA were evaluated in 15 patients by a branched DNAassay, the values decreased immediately after HDprocedure, but returned to the baseline values 2 days after the procedure. Conclusion These results indicate that HCVin HDpatients is replication-competent, although a transient reduction in the levels of HCV-RNA occurs during HD. (Internal Medicine 38: 626-631, 1999)

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“…HCV-RNA-positive samples were genotyped using InnoLiPA HCV II (Innogenetics, Zwijnaarde, Belgium) [10]. If the genotype could not be identified or if samples were HCV-RNA negative by Amplicor, we carried out an ‘in-house’ polymerase chain reaction (PCR) with 5′ non-coding (NC) biotinylated primers [11]. Thus all HCV-RNA-negative blood samples were tested twice.…”

Section: Methodsmentioning

confidence: 99%

Hepatitis C Virus: Routes of Infection and Genotypes in a Cohort of Anti-HCV-Positive French Blood Donors

Elghouzzi¹,

Bouchardeau²,

Pillonel³

et al. 2000

Vox Sanguinis

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Background: We evaluated and analysed risk factors of HCV-infected blood donors according to HCV genotypes in order to improve the transfusion policy and safety of blood supply. Materials and Methods: HCV-RNA was analysed in sera from 518 anti-HCV-positive blood donors, who were invited to medical consultation and interview as to risk factors by means of an extensive questionnaire. HCV genotyping was done on all samples positive for HCV-RNA. Results: Of the 518 sera, 399 (77%) were HCV-RNA positive, and 394 of 399 HCV genotypes were identified. Major genotypes were 1b (34.3%), 3a (24%), 1a (19.5%) and 2 (11.4%). Of the donors, 289 (55.8%) were interviewed regarding their risk behaviour: 27% were former intravenous drug users (IVDUs), 26% had been transfused, 8% had a history of invasive diagnostic procedures, and 13% a history of surgery. Among the 224 interviewed donors, genotypes 1a and 3a were mainly associated with IVDU (51 and 45% respectively) and genotype 1b, with transfusion and nosocomial infections (40 and 25%, respectively). Conclusion: In this population of anti-HCV-positive blood donors, nosocomial infection may be a route of HCV spread, but the main risk factor remains IVDU, particularly in young men. The transfusion policy will improve if predonation interviews of such young men are done with a specific and sensitive questionnaire.

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Detection of hepatitis C virus by polym erase chain reaction in haemodialysed patients in relationship to anti-HCV status

Cited by 13 publications

References 27 publications

Effect of hemodialysis schedules and membranes on hepatocyte growth factor and hepatitis C virus RNA levels

Effect of hemodialysis schedules and membranes on hepatocyte growth factor and hepatitis C virus RNA levels

Prevalence and Characterization of Hepatitis C Virus in Hemodialysis Patients.

Hepatitis C Virus: Routes of Infection and Genotypes in a Cohort of Anti-HCV-Positive French Blood Donors

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