Detection of hepatitis E virus genotype 3 in wastewater by an electrochemical genosensor

Alzate, Diana; Lopez-Osorio, Maria C.; Cortés-Mancera, Fabián; Navas, María Cristina; Orozco, Jahir

doi:10.1016/j.aca.2022.340121

Cited by 7 publications

(9 citation statements)

References 34 publications

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“…This event can be monitored in a reagentless manner by tagging one end of the probe with a redox-active label. After the hybridization, the change in the redox current is measured to quantify the specific target DNA sequence. − Based on this approach, various electrochemical genosensors have been developed. − Peptide nucleic acids (PNAs) and locked nucleic acids (LNAs) offer improved hybridization properties over nucleic acids and have also been explored as biorecognition element …”

Section: Biorecognition Elements and Signal Transductionmentioning

confidence: 99%

Advances in Bioelectrode Design for Developing Electrochemical Biosensors

Kalita,

Gogoi,

Minteer

et al. 2023

ACS Meas. Sci. Au

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The critical performance factors such as selectivity, sensitivity, operational and storage stability, and response time of electrochemical biosensors are governed mainly by the function of their key component, the bioelectrode. Suitable design and fabrication strategies of the bioelectrode interface are essential for realizing the requisite performance of the biosensors for their practical utility. A multifaceted attempt to achieve this goal is visible from the vast literature exploring effective strategies for preparing, immobilizing, and stabilizing biorecognition elements on the electrode surface and efficient transduction of biochemical signals into electrical ones (i.e., current, voltage, and impedance) through the bioelectrode interface with the aid of advanced materials and techniques. The commercial success of biosensors in modern society is also increasingly influenced by their size (and hence portability), multiplexing capability, and coupling in the interface of the wireless communication technology, which facilitates quick data transfer and linked decision-making processes in real-time in different areas such as healthcare, agriculture, food, and environmental applications. Therefore, fabrication of the bioelectrode involves careful selection and control of several parameters, including biorecognition elements, electrode materials, shape and size of the electrode, detection principles, and various fabrication strategies, including microscale and printing technologies. This review discusses recent trends in bioelectrode designs and fabrications for developing electrochemical biosensors. The discussions have been delineated into the types of biorecognition elements and their immobilization strategies, signal transduction approaches, commonly used advanced materials for electrode fabrication and techniques for fabricating the bioelectrodes, and device integration with modern electronic communication technology for developing electrochemical biosensors of commercial interest.

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Section: Biorecognition Elements and Signal Transductionmentioning

confidence: 99%

Advances in Bioelectrode Design for Developing Electrochemical Biosensors

Kalita,

Gogoi,

Minteer

et al. 2023

ACS Meas. Sci. Au

View full text Add to dashboard Cite

show abstract

“…Nevertheless, it is necessary to overcome the interference of complex target genomes during the design phase and the mismatch problem of short nucleotide chains as recognition molecules. To date, DNA sensors have been reported to detect HAV, HEV [ 44 , 45 ], and NoV [ 46 ] with the use of nanomaterials such as silver (Ag)/gold (Au)/magnetic nanoparticles or quantum dots (QDs).…”

Section: Current Biosensors For Waterborne Virus Detection In Human S...mentioning

confidence: 99%

“…Moreover, modify-free electrodes have stronger expansibility. Examples include the addition of magnetic nanoparticles (MNPs) to facilitate the rapid separation and enrichment of target analytes from complex samples, thereby significantly improving the sensitivity of bioassays and eliminating matrix interference [ 44 , 70 ]. Jiang et al.…”

Section: Current Biosensors For Waterborne Virus Detection In Human S...mentioning

confidence: 99%

“…3 F) [ 71 ]. Additionally, the modification-free system can achieve electrical signal amplification through various methods, such as sandwich detection [ 44 , 70 ], nucleic acid hybridization [ 45 , 72 ], and gene amplification [ 72 , 73 ], as further discussed in Section 3.5 .…”

Section: Current Biosensors For Waterborne Virus Detection In Human S...mentioning

confidence: 99%

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Biosensors for waterborne virus detection: Challenges and strategies

Song,

Fredj,

Zheng

et al. 2023

Journal of Pharmaceutical Analysis

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“…Electrochemical genosensors employing nanomaterials could provide detection with several benefits, such as great sensitivity and selectivity, early detection, celerity, and low-cost [ 7 , 8 ]. Reduced graphene oxide (rGO) advantages include a large specific surface area, high conductivity, and good stability [ 9 ].…”

Section: Introductionmentioning

confidence: 99%