Detection of hepatocellular carcinomas with near-infrared fluorescence imaging using indocyanine green: its usefulness and limitation

Morita, Yoshifumi; Sakaguchi, Takanori; Unno, Naoki; Shibasaki, Yasushi; Suzuki, Atsushi; Fukumoto, Koji; Inaba, Kazuaki; Baba, Satoshi; Takehara, Yasuo; Suzuki, Shinichi; Konno, Hiroyuki

doi:10.1007/s10147-011-0367-3

Cited by 96 publications

(68 citation statements)

References 19 publications

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“…For instance, ICG can clearly identify hepatocellular carcinoma lesions and colorectal cancer liver metastases during surgery. 147,148 Presently, intraoperative evaluation of tumor margins depends on palpation and visual inspection. NIRF probes with cancertargeting ability can provide instrumental information in demarcating tumors.…”

Section: Nirf Probes In Cancer Imagingmentioning

confidence: 99%

Near-infrared fluorescent probes in cancer imaging and therapy: an emerging field

Yi¹,

Wang²,

Qin³

et al. 2014

IJN

213

149

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Near-infrared fluorescence (NIRF) imaging is an attractive modality for early cancer detection with high sensitivity and multi-detection capability. Due to convenient modification by conjugating with moieties of interests, NIRF probes are ideal candidates for cancer targeted imaging. Additionally, the combinatory application of NIRF imaging and other imaging modalities that can delineate anatomical structures extends fluorometric determination of biomedical information. Moreover, nanoparticles loaded with NIRF dyes and anticancer agents contribute to the synergistic management of cancer, which integrates the advantage of imaging and therapeutic functions to achieve the ultimate goal of simultaneous diagnosis and treatment. Appropriate probe design with targeting moieties can retain the original properties of NIRF and pharmacokinetics. In recent years, great efforts have been made to develop new NIRF probes with better photostability and strong fluorescence emission, leading to the discovery of numerous novel NIRF probes with fine photophysical properties. Some of these probes exhibit tumoricidal activities upon light radiation, which holds great promise in photothermal therapy, photodynamic therapy, and photoimmunotherapy. This review aims to provide a timely and concise update on emerging NIRF dyes and multifunctional agents. Their potential uses as agents for cancer specific imaging, lymph node mapping, and therapeutics are included. Recent advances of NIRF dyes in clinical use are also summarized.

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Section: Nirf Probes In Cancer Imagingmentioning

confidence: 99%

Near-infrared fluorescent probes in cancer imaging and therapy: an emerging field

Yi¹,

Wang²,

Qin³

et al. 2014

IJN

213

149

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“…Intravenously administered ICG has also been used in NIR endoscopy systems to successfully differentiate superficial gastric tumors from invasive cancers (13). ICG has been shown to localize to both hepatocellular carcinoma and intrahepatic metastases 24-72 hours after injection with high target-to-background ratios (2,3,6). The mechanism by which ICG localizes to intrahepatic metastases has recently begun to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

“…The intraoperative imaging of ICG for the detection of intrahepatic malignancy has been demonstrated (2,3,6). Indeed, OMI may add value to standard visual inspection and intraoperative ultrasonography (US) for the detection of superficial hepatic lesions during surgical resection (7).…”

Section: Molecular Imaging: Interventional Optical Molecular Imaging mentioning

confidence: 99%

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Interventional Optical Molecular Imaging Guidance during Percutaneous Biopsy

Sheth¹,

Heidari²,

Wood³

et al. 2014

Radiology

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Purpose:To investigate indocyanine green (ICG) as a molecular beacon for malignant lesions within the liver and evaluate the ability of a developed handheld imaging system to allow measurement of ICG fluorescence within focal hepatic lesions with high target-to-background ratios in a mouse model. Materials and Methods:All animal experiments were approved by the institutional animal care committee. A handheld optical molecular imaging device was constructed to pass through the introducer needle of a standard percutaneous biopsy kit. An ex vivo phantom system was constructed to quantify tissue attenuation properties of ICG in liver parenchyma. Subsequently, intrahepatic colorectal cancer metastases were generated in nude mice, and epifluorescence imaging of ICG, as well as histologic analysis of the explanted livers, was performed at 3 weeks after implantation (n = 6). Epifluorescence imaging with the handheld imaging device was then performed on intrahepatic colorectal metastases after the administration of ICG (n = 15) at 3, 6, and 24 hours after injection. Target-to-background ratios were calculated for each time point. Subsequently, a core biopsy of intrahepatic colorectal metastases was performed by using a standard clinical 18-gauge biopsy needle. Results:There was avid localization of ICG to the focal lesions at all time points. Similarly, fluorescence within the tumors was greater than that within normal liver, as detected with the handheld imaging system (mean target-to-background ratio 6 standard deviation, 3.9 6 0.2 at 24 hours). A core biopsy of tumor and normal adjacent liver by using a standard biopsy needle demonstrated a sharp margin of fluorescence intensity at the tumor-liver interface. Conclusion:The custom-designed molecular imaging device, in combination with ICG, readily allowed differentiation between normal and malignant tissue in a murine model of intrahepatic colorectal metastasis.q RSNA, 2014

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“…PDT requires a chemical agent that is called as photosensitizer and activation of the agent by light of a specific wavelength to produce oxygendependent cytotoxic reaction [1][2][3] Indocyanine green (ICG), a photosensitizer with a molecular weight of 775 Da, has been used as a diagnostic agent to determine cardiac output, hepatic function and blood flow [4,5]. ICG has low toxicity and has been approved by Food and Drug Administration (FDA) [6].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effects of Different ICG Concentrations and Laser Parameters on Neuroblastoma

Kaya

Coşan

et al. 2015

Acta Phys. Pol. A

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Photodynamic therapy (PDT) is a minimally invasive treatment for cancer therapy. It can be administered in combination with other treatments such as chemotherapy, radiotherapy, and surgical excision. PDT involves a photosensitizing agent that is activated by exposure to a specific wavelength of light. PDT is a cold photochemical process, there is no tissue heating. In our study, we investigated whether different laser parameters with different concentrations of indocyanine green (ICG) have cytotoxic and anti-proliferative effects on neuroblastoma. Plates were divided groups as control, only ICG concentrations (25 and 50 µg/ml), only laser treatment I (50 J/cm 2 ), only laser treatment II (100 J/cm 2 ), 25 µg/ml ICG + laser treatment I and 25 µg/ml ICG + laser treatment II, 50 µg/ml ICG + laser treatment I and 50 µg/ml ICG + laser treatment II. Neuroblastoma cell lines were irradiated with an in-house developed diode laser system (λ = 809 nm, 70 mW/cm 2 , 50 & 100 J/cm 2 ) in continuous wave operation mode after ICG application. Cell proliferation was measured by XTT assay after light irradiation. Cell proliferation was decreased in a dose-dependent manner in 25 and 50 µg/ml ICG concentrations when compared with control. The applied ICG concentrations (especially 50 µg/ml) had cytotoxic effects for neuroblastoma cell lines, SH-SY5Y. There was no difference between laser treatment groups (L 50 & 100 J/cm 2 ). However, PDT groups (laser exposure with ICG) showed significant inhibition of cell viability (p < 0.05). Additionally, laser exposure did not increase the well temperature above the incubation parameter. In conclusion, PDT has cytotoxic effects in neuroblastoma cell lines. Appropriate ICG dose -laser parameter combinations must be determined for each cell type. Different energy densities may cause different effects of PDT on inhibition of cell viability.

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Detection of hepatocellular carcinomas with near-infrared fluorescence imaging using indocyanine green: its usefulness and limitation

Abstract: Indocyanine green fluorography is useful to detect HCCs; however, attention should be paid to the fact that HCCs may be occasionally overlooked by this imaging method and that lesions detected by this method are not always neoplastic lesions.

Cited by 96 publications

References 19 publications

Near-infrared fluorescent probes in cancer imaging and therapy: an emerging field

Near-infrared fluorescent probes in cancer imaging and therapy: an emerging field

Interventional Optical Molecular Imaging Guidance during Percutaneous Biopsy

Cytotoxic Effects of Different ICG Concentrations and Laser Parameters on Neuroblastoma

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