Detection of herpes viruses in children with acute appendicitis

Katzoli, P; Sakellaris, George; Ergazaki, M.; Charissis, G.; Spandidos, Demetrios Α.; Sourvinos, George

doi:10.1016/j.jcv.2009.01.013

Cited by 29 publications

(16 citation statements)

References 32 publications

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“…There has been a long standing suspicion that non-HHV viral infections are associated with appendicitis, given it’s bimodal and seasonal occurrence (158). The first described association of HHV with appendicitis was in an AIDS patient (159), but a recent study of childhood appendicitis has shown periodic reactivation (21%) of CMV in lymphoid tissue of the appendix (160). In this study, HHV-6 was also identified in about 8% of specimens.…”

Section: Virusesmentioning

confidence: 99%

Resistant Pathogens, Fungi, and Viruses

Guidry

Mansfield

Sawyer

et al. 2014

Surgical Clinics of North America

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The first reports of antibiotic pathogens occurred a few short years after the introduction of these powerful new agents, heralding a new kind of war between medicine and pathogens. Although originally described in Staphylococcus aureus, resistance among bacteria has now become a grim race to determine which classes of bacteria will become more resistant, pitting the Gram positive staphylococci, enterococci, and streptococci against the increasingly resistant Gram negative pathogens, e. g., carbapenemase-resistant enterobacteriaceae. In addition, the availability of antibacterial agents has allowed the development of whole new kinds of diseases caused by non-bacterial pathogens, related largely to fungi that are inherently resistant to antibacterials. All of these organisms are becoming more prevalent and, ultimately, more clinically relevant for surgeons. It is ironic that despite their ubiquity in our communities, there is seldom a second thought given to viral infections in patients with surgical illness. The extent of most surgeon’s interest in viral infections ends with hepatitis and HIV, no doubt related to transmissibility as well as the implications that these viruses might have in a patient’s hepatic or immune functions. There are chapters and even textbooks written about these viruses so these will not be considered here. Instead, we will present the growing body of knowledge of the herpes family viruses and their occurrence and consequences in patients with concomitant surgical disease or critical illness. We have also chosen to focus this chapter on previously immune competent patients, as the impact of herpes family viruses in immunosuppressed patients such as transplant or AIDS patients has received thorough treatment elsewhere.

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Section: Virusesmentioning

confidence: 99%

Resistant Pathogens, Fungi, and Viruses

Guidry

Mansfield

Sawyer

et al. 2014

Surgical Clinics of North America

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“…4 HHV-6'nın akut apandisit ve aortta kronik arterite yol açtığına dair yayınlar da bildirilmiştir. 26,27 Ayrıca, HHV-6 reaktivasyonu ile skleroderma arasında güçlü bir ilişki olduğu öne sürülmekte-dir. 28 HHV-6 için tanısal testler seroloji, kültür, immünohistokimya ve nükleik asit incelemelerinden ibarettir.…”

Section: Human Herpes Vi̇rüs-6unclassified

Dermatological Skin Diseases Associated with Human Herpes Viruses 6, 7 and 8: Review

Adışen¹,

Aksoy²,

Aksakal³

et al. 2016

Turkiye Klinikleri J Dermatol

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“…The presence of bacteria within the peritoneal cavity during inflammatory processes was demonstrated at the beginning of the past century and the bacteria involved have been subsequently found, the most common ones being Escherichia coli, followed by Klebsiella pneumoniae, Streptococcus species, Enterococcus species and Pseudomonas aeruginosa. 1,2 This led to the adjustment of post-operative empirical antibiotic treatment, [3][4][5][6][7][8][9][10] although with the advent of broad spectrum antibiotics, routinary microbiological cultures of abdominal fluid specimens lost most of their importance. 11,12 Moreover, a specific disadvantage of cultures was that both pathogen identification and antibiotic sensitivity results were often available only after patients discharge.…”

Section: Introductionmentioning

confidence: 99%

The Role of DNA Amplification and Cultural Growth in Complicated Acute Appendicitis

et al. 2016

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Bacterial growth of peritoneal fluid specimens obtained during surgical procedures for acute appendicitis may be useful to optimize further antibiotic therapy in complicated cases. DNA amplification represents a fast technique to detect microbial sequences. We aimed to compare the potential of DNA amplification versus traditional bacterial growth culture highlighting advantages and drawbacks in a surgical setting. Peritoneal fluid specimens were collected during surgery from 36 children who underwent appendectomy between May and December 2012. Real-time polymerase chain reaction (RT-PCR) and cultures were performed on each sample. RT-PCR showed an amplification of 16S in 18/36 samples, Escherichia coli (in 7 cases), Pseudomonas aeruginosa (3), Fusobacterium necrophorum (3), Adenovirus (2), E.coli (1), Klebsiella pneumoniae (1), Serratia marcescens/Enterobacter cloacae (1). Bacterial growth was instead observed only in four patients (3 E.coli and 1 P.aeruginosa and Bacteroides ovatus). Preoperative C-reactive protein and inflammation degree, the most reliable indicators of bacterial translocation, were elevated as expected. DNA amplification was a quick and useful method to detect pathogens and it was even more valuable in detecting aggressive pathogens such as anaerobes, difficult to preserve in biological cultures; its drawbacks were the lack of biological growths and of antibiograms. In our pilot study RT-PCR and cultures did not influence the way patients were treated.

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Detection of herpes viruses in children with acute appendicitis

Cited by 29 publications

References 32 publications

Resistant Pathogens, Fungi, and Viruses

Resistant Pathogens, Fungi, and Viruses

Dermatological Skin Diseases Associated with Human Herpes Viruses 6, 7 and 8: Review

The Role of DNA Amplification and Cultural Growth in Complicated Acute Appendicitis

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