Detection of herpesviruses in neotropical primates from São Paulo, Brazil

Furusato, Isabella Naomi; Figueiredo, Ketlyn Bolsachini; de Carvalho, Ana Carolina Souza Ramos; da Silva Ferreira, Camila Santos; Takahashi, Juliana Possatto Fernandes; Kimura, Lidia Midori; Aleixo, Camila Siqueira; de Brito, Odília Pereira; Luchs, Adriana; Cunha, Mariana Sequetin; de Azevedo Fernandes, Natália Coelho Couto; de Araújo, Leonardo José Tadeu; Catão-Dias, José Luiz; Guerra, Juliana Mariotti

doi:10.1007/s42770-023-01105-z

Cited by 1 publication

(1 citation statement)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Total DNA was extracted from the AGM samples using the QIAamp DNA Mini Kit (Qiagen Sciences, Germantown, MD, USA) following the manufacturer’s instructions. The samples were screened for the presence of herpesviruses by a broad-spectrum nested-PCR assay (targeting a short stretch (~215–315 bp) of the herpesvirus DPOL gene) that has been successfully used to detect alpha-, beta- and/or gammaherpesviruses in NHPs and various other host species, including identification of novel herpesviruses [ 20 , 49 , 58 , 59 ]. Primers employed in PCRs/Semi-nested PCRs/Nested PCRs to determine the partial DPOL and gB CDS of the AGM herpesviruses were designed in the present study and have been shown in Supplementary Table S1 .…”

Section: Methodsmentioning

confidence: 99%

Molecular Detection and Genetic Diversity of Cytomegaloviruses and Lymphocryptoviruses in Free-Roaming and Captive African Green Monkeys (Chlorocebus sabaeus)

Mancuso,

Gainor,

Dore

et al. 2024

IJMS

View full text Add to dashboard Cite

To date, limited information is available on cytomegalovirus (CMV) and lymphocryptovirus (LCV) from Chlorocebus monkeys. We report here high detection rates of herpesviruses in free-roaming African green monkeys (AGMs, Chlorocebus sabaeus) (26.4%, 23/87) and in captive AGMs (75%, 3/4) with respiratory disease on the Caribbean Island of St. Kitts. LCV (81.25%) was more prevalent than CMV (18.75%) in the AGMs. Applying a bigenic PCR approach (targeting DNA polymerase (DPOL) and glycoprotein B (gB) genes), long sequences were obtained from representative AGM CMV (KNA-SD6) and LCV (KNA-E4, -N6 and -R15) samples, and mixed LCV infections were identified in KNA-N6 and -R15. The nucleotide (nt) sequence (partial DPOL-intergenic region-partial gB) and partial DPOL- and gB-amino acid (aa) sequences of AGM CMV KNA-SD6 were closely related to Cytomegalovirus cercopithecinebeta5 isolates from grivet monkeys, whilst those of AGM LCV KNA-E4 and -N6 (and E4-like gB of KNA-R15) were more closely related to cognate sequences of erythrocebus patas LCV1 from patas monkey than other LCVs, corroborating the concept of cospeciation in the evolution of CMV/LCV. On the other hand, the partial DPOL aa sequence of KNA-R15, and additional gB sequences (N6-gB-2 and R15-gB-2) from samples KNA-N6 and -R15 (respectively) appeared to be distinct from those of Old World monkey LCVs, indicating LCV evolutionary patterns that were not synchronous with those of host species. The present study is the first to report the molecular prevalence and genetic diversity of CMV/LCV from free-roaming/wild and captive AGMs, and is the first report on analysis of CMV nt/deduced aa sequences from AGMs and LCV gB sequences from Chlorocebus monkeys.

show abstract