Detection of HHV‐8 (human herpesvirus‐8) genomes in induced peripheral blood mononuclear cells (PBMCs) from US blood donors

Qu, Lirong; Triulzi, Darrell J.; Rowe, David; Jenkins, Frank J.

doi:10.1111/j.1423-0410.2010.01404.x

Cited by 7 publications

(7 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More recently, Qu et al [6], testing purified DNA equivalent to more than 3×10 4 B-cells from 861 donors, including 40 samples from HHV-8 seropositive individuals, did not detect HHV-8 DNA in any of these samples. Furthermore, HHV-8 genomes were not detected from induced/cultured PBMCs and CD19+ B-cells from 164 blood donors, including seven HHV-8 seropositive samples [24]. Unfortunately, in our work, we could not isolate PBMCs from whole blood samples, as repository samples were immediately frozen after whole blood collection at the blood bank.…”

Section: Discussionmentioning

confidence: 88%

Non-Detection of Human Herpesvirus 8 (HHV-8) DNA in HHV-8-Seropositive Blood Donors from Three Brazilian Regions

et al. 2011

View full text Add to dashboard Cite

Human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma-associated herpesvirus (KSHV), is the etiologic agent of all forms of Kaposi's sarcoma, primary effusion lymphoma and the plasmablastic cell variant of multicentric Castleman disease. In endemic areas of sub-Saharan Africa, blood transfusions have been associated with a substantial risk of HHV-8 transmission. By contrast, several studies among healthy blood donors from North America have failed to detect HHV-8 DNA in samples of seropositive individuals. In this study, using a real-time PCR assay, we investigated the presence of HHV-8 DNA in whole-blood samples of 803 HHV-8 blood donors from three Brazilian states (São Paulo, Amazon, Bahia) who tested positive for HHV-8 antibodies, in a previous multicenter study. HHV-8 DNA was not detected in any sample. Our findings do not support the introduction of routine HHV-8 screening among healthy blood donors in Brazil. (WC = 140).

show abstract

Section: Discussionmentioning

confidence: 88%

Non-Detection of Human Herpesvirus 8 (HHV-8) DNA in HHV-8-Seropositive Blood Donors from Three Brazilian Regions

et al. 2011

View full text Add to dashboard Cite

show abstract

“…A third study induced peripheral blood mononuclear cells and CD19+ B cells from 164 blood donors and no HHV-8 was detected indicating the level of HHV-8 in blood donors is extremely low, if present at all. 11 Limitations of our study include small sample size and the inability to measure efficacy of leukoreduction filtration on blood from healthier study subjects without KS since none of them had detectable HHV-8 DNA.…”

Section: Discussionmentioning

confidence: 99%

Measurements of human herpesvirus 8 viral load in blood before and after leukoreduction filtration

et al. 2013

View full text Add to dashboard Cite

show abstract

“…For the in vitro experiments, a total of 5×10 5 B cells and 500 µl of culture supernatant were collected post-HHV-8 exposure, pelleted, and assayed by PCR (50, 51). Samples were treated with 1 µl DNase in 10 µl buffer (Sigma) for 15 min and then lysed in easyMAG buffer (NucliSENS).…”

Section: Methodsmentioning

confidence: 99%

“…Phocine herpesvirus was added as an internal control (52). DNA was mixed with a primer set specific for HHV-8 K8.1 (51), and the real-time PCR was done using a 7000, 7500, or ViiA7 ABI system (Applied Biosystems). B cells isolated from PBMC obtained from KS cases and controls were treated and tested for HHV-8 DNA as described above.…”

Section: Methodsmentioning

confidence: 99%

Human Herpesvirus 8 Induces Polyfunctional B Lymphocytes That Drive Kaposi’s Sarcoma

Knowlton

Rappocciolo

Piazza

et al. 2014

mBio

View full text Add to dashboard Cite

Kaposi’s sarcoma (KS) is an unusual neoplasia wherein the tumor consists primarily of endothelial cells infected with human herpesvirus 8 (HHV-8; Kaposi’s sarcoma-associated herpesvirus) that are not fully transformed but are instead driven to excess proliferation by inflammatory and angiogenic factors. This oncogenic process has been postulated but unproven to depend on a paracrine effect of an abnormal excess of host cytokines and chemokines produced by HHV-8-infected B lymphocytes. Using newly developed measures for intracellular detection of lytic cycle proteins and expression of cytokines and chemokines, we show that HHV-8 targets a range of naive B cell, IgM memory B cell, and plasma cell-like populations for infection and induction of interleukin-6, tumor necrosis factor alpha, macrophage inhibitory protein 1α, macrophage inhibitory protein 1β, and interleukin-8 in vitro and in the blood of HHV-8/HIV-1-coinfected subjects with KS. These B cell lineage subsets that support HHV-8 infection are highly polyfunctional, producing combinations of 2 to 5 of these cytokines and chemokines, with greater numbers in the blood of subjects with KS than in those without KS. Our study provides a new paradigm of B cell polyfunctionality and supports a key role for B cell-derived cytokines and chemokines produced during HHV-8 infection in the development of KS.

show abstract

Detection of HHV‐8 (human herpesvirus‐8) genomes in induced peripheral blood mononuclear cells (PBMCs) from US blood donors

Abstract: HHV-8 genomes were not detected from PBMCs, induced/cultured PBMCs and CD19+ B cells from 164 blood donors. The level of detectable HHV-8 genomes in blood donors seems to be extremely low, if they exist.

Cited by 7 publications

References 21 publications

Non-Detection of Human Herpesvirus 8 (HHV-8) DNA in HHV-8-Seropositive Blood Donors from Three Brazilian Regions

Non-Detection of Human Herpesvirus 8 (HHV-8) DNA in HHV-8-Seropositive Blood Donors from Three Brazilian Regions

Measurements of human herpesvirus 8 viral load in blood before and after leukoreduction filtration

Human Herpesvirus 8 Induces Polyfunctional B Lymphocytes That Drive Kaposi’s Sarcoma

Contact Info

Product

Resources

About