Detection of High- and Low-Risk HPV DNA in Archived Breast Carcinoma Tissues from Ethiopian Women

Gebregzabher, Endale; Seifu, Daniel; Tigneh, Wondemagegnhu; Bokretsion, Yonas; Bekele, Abebe; Abebe, Markos; Lillsunde-Larsson, Gabriella; Karlsson, Christina; Karlsson, Mats G.

doi:10.1155/2021/2140151

Cited by 7 publications

(3 citation statements)

References 22 publications

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“…Non-malignant breast tumors had a higher HPV percentage (29.6%) than breast cancer (20.6%), which may suggest that there is no association between HPV infection and breast cancer. Another earlier study found that HPV was present in 2.7% of Ethiopian breast cancer cases [34] which is a far lower number than the current study. The percentage of HPV positivity was higher in…”

Section: Discussioncontrasting

confidence: 87%

High rate of high-risk human papillomavirus among benign and breast cancer patients in Ethiopia

Belachew,

Desta,

Mulu

et al. 2024

PLoS ONE

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Introduction There have been numerous studies that showed the presence of human papillomavirus (HPV) in breast cancer; nonetheless, there is ongoing debate regarding their association. Given few studies in Ethiopia, we aimed to investigate the magnitude of HPV infection in Ethiopian breast cancer patients. Methods A total of 120 formalin-fixed paraffin-embedded (FFPE) tissue blocks were obtained, and basic demographic, clinical, and histological data were collected from medical records. DNA was extracted from archived FFPE breast tissue specimens using GeneRead DNA FFPE Kit. The AnyplexTM II HPV28 Detection Kit (Seegene, Korea) was used to detect HPV by following the manufacturer’s instructions. The SPSS Version 25 was used to enter and analyze data. Results Among the 120 study participants; HPV (both high-risk and low-risk) was detected in 20.6% of breast cancer and 29.6% of non-malignant breast tumors. The most common genotype was the high-risk HPV 16 genotype. The frequency of HPV was nearly 10-fold higher in estrogen receptor-positive than ER-negative breast cancer. The percentage of HPV in the luminal (luminal A and luminal B) breast cancer subtypes was also much higher than in the non-luminal subtypes (HER-2 enriched and triple-negative breast cancer). Conclusion This study did not find a significant difference in HPV expression between breast cancer and non-malignant breast tumors; however, the higher percentage of HPV in ER-positive compared to ER-negative breast cancer warrants further attention.

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Section: Discussioncontrasting

confidence: 87%

High rate of high-risk human papillomavirus among benign and breast cancer patients in Ethiopia

Belachew,

Desta,

Mulu

et al. 2024

PLoS ONE

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“…HPV genotypes 16 and 18 are the most common virus types found in cancers worldwide ( 32 ). However, other types of HPV including 6, 11, 33, 35, 39, 45, 51 and 59 have also been detected in breast cancer patients ( 33 ). Two meta-analyses showed a higher prevalence of high-risk HPVs, including 16, 18 and 33, compared to other HPV strains.…”

Section: Discussionmentioning

confidence: 99%

Low presence of papillomavirus and its lack of correlation with clinicopathological factors in breast cancer: a case control study

Makvandi,

Rashno,

Faghihloo

et al. 2023

IJM

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Background and Objectives: Breast cancer is currently the most commonly diagnosed neoplasm in women worldwide. There is evidence that human papillomavirus (HPV) infection may play a key role in breast cancer aggressiveness, but results are conflicting across studies. The aim of this study was to investigate the presence of the HPV viral genome in benign and malignant breast tissue samples and its clinicopathological characteristics of cancer. Materials and Methods: In this case-control study, 100 formalin-fixed paraffin-embedded (FFPE) of breast cancer and 100 blocks of non-cancerous breast tissue were selected as a control group from the pathology department of Imam Khomeini Hospital in Ahvaz from 2020-2022. The presence of HPV was detected using nested PCR including MY09/11 primers and sequencing were performed for virus genotyping. Results: The present study enrolled 100 subjects each in two cancer and control groups with a mean age of 52.81±13.23 and 35.77±11.65, respectively. The risk of cancer in HPV-infected patients is almost 5 times higher than in HPV-negative individuals, it is not statistically significant (OR =4.99, 95% CI 0.35 to 72.15, p=0.238). The prevalence of HPV in the cancer and control groups was 7% and 1%, respectively and HPVs detected in two groups were of the HPV 16 genotype. Although the chance of ER and PR expression, lymphvascular involvement, perineural invasion, and higher tumor grade was higher in HPV-positive subjects than in HPV-negative subjects, this was not statistically significant (OR>1, p>0.05). Conclusion: Based on studies reporting the existence of sequences of different high-risk HPV types (oncogenes) in breast cancer tissues, this study confirmed the hypothesis of a possible infectious cause in the development of breast cancer. So far, however, the results have been controversial and inconclusive. Further studies with large sample sizes are needed to demon- strate the link between HPV and breast cancer.

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“…Viral etiology of breast carcinoma has been addressed in numerous studies, but still remains controversial ( 9 ). Several viruses, including bovine leukemia virus, Kaposi's sarcoma-associated herpesvirus, Epstein-Barr virus (EBV), mouse mammary tumor virus (MMTV), simian vacuolating virus 40 ( 10 ), human mammary tumor virus ( 11 ), cytomegalovirus (CMV) ( 12 ), herpes simplex virus-1 (HSV), human herpes virus type-8 ( 13 ) have been regarded as potential breast oncogenic factors a number of years ago. In 1944, the discovery that MMTV caused BC in mice led researchers to investigate a possible viral contribution to BC ( 14 ).…”

Section: Introductionmentioning

confidence: 99%

Human papillomaviruses and breast cancer: A systematic review and meta‑analysis

Karachalios,

Petousis,

Margioula‑Siarkou

et al. 2023

Oncol Lett

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Breast cancer (BC) is the leading malignancy worldwide. The association between human papillomavirus (HPV) and BC is debatable. The present systematic review and meta-analysis aimed to assess the prevalence of HPV DNA in malignant breast tumors. An extensive search of the PubMed and SCOPUS databases was carried out for case-control studies published between January 1, 2003 and January 7, 2023, which compared HPV DNA detection in breast tissue specimens of female patients with BC and women with absent or benign breast disorders. Once the initial title/abstract screening was completed by two independent investigators, the full texts of the included studies from that stage were reviewed by the aforementioned investigators to determine if they should be included in the present study. Data extraction was independently conducted by two investigators. A third investigator was consulted to resolve disagreements through free discussion. MedCalc was used for quantitative synthesis. The significance of association was estimated by pooled odds ratios (ORs) with 95% confidence intervals (CIs) calculated using the random-effects model. A total of 23 primary studies, including 3,243 subjects (2,027 patients and 1,216 controls), were eligible for quantitative analysis. HPV prevalence in patients with BC and controls was 21.95 and 8.96%, respectively. The prevalence of HPV differed significantly between the two groups (OR 3.83; 95% CI 2.03-7.25; P<0.01). Heterogeneity among studies was quantified using the I 2 index which was 69.57% (95% CI 51.89-80.75). The risk of bias was assessed using an appropriate tool contributed by the CLARITY Group at McMaster University. Seven studies had a low risk of bias, 15 studies had a moderate risk of bias and only one study had a serious risk of bias. These results reinforce the hypothesis that HPV is involved in BC development and progression, indicating a possible role of HPV vaccination in BC prevention.

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Detection of High- and Low-Risk HPV DNA in Archived Breast Carcinoma Tissues from Ethiopian Women

Cited by 7 publications

References 22 publications

High rate of high-risk human papillomavirus among benign and breast cancer patients in Ethiopia

High rate of high-risk human papillomavirus among benign and breast cancer patients in Ethiopia

Low presence of papillomavirus and its lack of correlation with clinicopathological factors in breast cancer: a case control study

Human papillomaviruses and breast cancer: A systematic review and meta‑analysis

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