Detection of high levels of canine herpes virus-1 neutralising antibody in kennel dogs using a novel serum neutralisation test

Reading, Mark; Field, Hugh J.

doi:10.1053/rvsc.1998.0222

Cited by 22 publications

(16 citation statements)

References 8 publications

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“…In contrast to other studies suggesting that ELISA is more sensitive than SNT [15] and [19], this study shows that, at the dog level as well as the level of kennels, the seroprevalences obtained with SNT and ELISA were similar, which is in agreement with the findings of Reading and Field [20]. In the current study there was an almost perfect agreement between the results obtained by the SNT and the ELISA tests.…”

Section: Discussionsupporting

confidence: 85%

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Seroprevalence of canine herpesvirus in breeding kennels in the Gauteng Province of South Africa

et al. 2008

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Canine herpesvirus (CHV-1) causes neonatal deaths as well as infertility due to embryonal death, abortion and stillbirths in breeding kennels. The aim of this study was to determine the prevalence of antibodies against canine herpesvirus in the serum of dogs older than 1 year in breeding kennels in the Gauteng Province of South Africa. A serum neutralization test (SNT) and a newly developed enzyme linked immunosorbent assay (ELISA) were used to test the serum samples of 328 dogs in 38 breeding kennels. With SNT as well as ELISA, 22% of sera were positive (P > 0.9). Seventeen kennels (45% of total kennels) each had at least one positive dog on SNT compared with twenty kennels (53% of total kennels) that each had at least one positive dog on ELISA (P = 0.6). The prevalence of positive dogs in positive kennels was 42 ± 26% (n = 17 kennels) for SNT and 39 ± 26% (n = 20 kennels) for ELISA. Pairwise comparison of kennels showed that the prevalence of SNT positive dogs was similar to the prevalence of ELISA positive dogs (P = 0.3, n = 38 kennels). Seroprevalence was independent of age, gender or colony size. This study suggests that canine herpesvirus is sufficiently common in breeding dogs in the Gauteng Province of South Africa to pose a threat to neonatal survival and fertility.

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Section: Discussionsupporting

confidence: 85%

“…ELISA tests seem more sensitive than SNT [15] and [19] although Reading and Field [20] reported an SNT that had similar sensitivity to the ELISA they used.…”

Section: Introductionmentioning

confidence: 97%

Seroprevalence of canine herpesvirus in breeding kennels in the Gauteng Province of South Africa

et al. 2008

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“…Several serological investigations have revealed the prevalence of CaHV-1 infections in household and colony breeding dogs in various countries at different time points, such as USA (12.8% during 2003-2004) [6], Italy (27.9%, assuming before 1998) [30], Netherlands (39.3% during 1997-1998) [31], Belgium (45.8% in 2000) [32] and England (88% in 1994) [33]. In Asia, a CaHV-1 serological survey was fi rst reported in Korea and ranged from 18-70% depending on the region [34].…”

Section: Discussionmentioning

confidence: 99%

Viral molecular and pathological investigations ofCanid herpesvirus1 infection associated respiratory disease and acute death in dogs

et al. 2017

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Canid herpesvirus 1 (CaHV-1) is a member of the canine infectious respiratory disease complex (CIRDC). The outcome of CaHV-1 infection can be occasionally fatal. So far, no information on CaHV-1 circulation in Thailand has been reported resulting in a lack of preventive strategies. In this study, nasal (NS) and oropharyngeal (OS) swabs were collected from 100 live dogs with respiratory distress. Among them, 23 pleural effusions were aspirated. A panel of CIRDC-associated viruses was screened by (RT)-PCR, including CaHV-1, CIV, CPIV, CDV, CRCoV and CAdV-2, for all collected samples. The CaHV-1 was detected in 32 dogs. Additionally, CaHV-1 was consistently detected in six pleural effusions. Most CaHV-1 infected dogs were over 5 years of age (43.8%) and expressed a mild nasal discharge. Pathological results of four three-month-old puppies, naturally moribund from respiratory disease, revealed a severe multifocal necrotic-hemorrhagic disease in several organs without pathognomonic inclusion bodies. They were only found to be CaHV-1 positive by PCR. Phylogenetic analysis demonstrated concordant results of CaHV-1 circulation in Thailand. Although mostly found as a co-infection with other CIRDC viruses (68.8%) it also occurred alone. Therefore, rapid ante-mortem diagnosis might facilitate the investigation of unclassical CaHV-1 infection, which is fatal in neonates and causes illness in annually core-vaccinated adults.

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“…The prevalence of antibodies in dogs was 88% in England, 45.8% in Belgium, and 39.3% in the Netherlands [129,133,135]. Serological studies in Italy have revealed a similar prevalence in kennelled dogs (27.9%), while the prevalence was lower in pets (3.1%) [142].…”

Section: Canine Herpesvirusmentioning

confidence: 99%

Canine respiratory viruses

2007

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-Acute contagious respiratory disease (kennel cough) is commonly described in dogs worldwide. The disease appears to be multifactorial and a number of viral and bacterial pathogens have been reported as potential aetiological agents, including canine parainfluenza virus, canine adenovirus and Bordetella bronchiseptica, as well as mycoplasmas, Streptococcus equi subsp. zooepidemicus, canine herpesvirus and reovirus-1,-2 and -3. Enhancement of pathogenicity by multiple infections can result in more severe clinical forms. In addition, acute respiratory diseases associated with infection by influenza A virus, and group I and II coronaviruses, have been described recently in dogs. Host species shifts and tropism changes are likely responsible for the onset of these new pathogens. The importance of the viral agents in the kennel cough complex is discussed.

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Detection of high levels of canine herpes virus-1 neutralising antibody in kennel dogs using a novel serum neutralisation test

Cited by 22 publications

References 8 publications

Seroprevalence of canine herpesvirus in breeding kennels in the Gauteng Province of South Africa

Seroprevalence of canine herpesvirus in breeding kennels in the Gauteng Province of South Africa

Viral molecular and pathological investigations ofCanid herpesvirus1 infection associated respiratory disease and acute death in dogs

Canine respiratory viruses

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