Detection of high-risk human papillomavirus by two molecular techniques: Hybrid capture and linear array

Antonio, Jesús; Fernández-Olmos, Ana; Mercadillo, María; Lindemann, María Luisa Mateos; Mochales, Fernando Baquero

doi:10.1016/j.jviromet.2008.01.021

Cited by 11 publications

(13 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Specifically, 8 of 15 specimens that were HR HPV negative and LR HPV positive by hc2 were subsequently found to have HR genotypes by LA. This is not surprising, as previous studies comparing hc2 and LA have reported a slightly higher sensitivity, but lower specificity, with the LA (11,14,20,28).…”

Section: Discussionsupporting

confidence: 64%

“…The LA has previously demonstrated sensitivity comparable to that of the hc2 (11,14,20,28) and was used in this study to resolve discordant hc2 and c4800 results and confirm paired-positive results. Total nucleic acid was extracted from 400 l of LBC specimen and eluted into 110 l using the easyMAG extractor (bioMérieux Canada Inc., Québec, Canada).…”

Section: Methodsmentioning

confidence: 95%

“…High-risk HPV testing (when used as either primary screening or for reflex testing following a nonnegative cytology result) improves patient management by providing a more accurate risk assessment for cervical cancer or its precursors (9,11,14,20,22,24). The first HR HPV test approved by the U.S. Food and Drug Administration (FDA) was the Hybrid Capture 2 (hc2) high-risk HPV DNA test (Qiagen, Mississauga, Ontario, Canada), which detects HPV by hybridization of genotype-specific RNA probes to denatured viral DNA.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Comparison of the Hybrid Capture 2 and cobas 4800 Tests for Detection of High-Risk Human Papillomavirus in Specimens Collected in PreservCyt Medium

et al. 2012

View full text Add to dashboard Cite

Clinical cervical cytology specimens (n ‫؍‬ 466) collected in PreservCyt (Hologic Inc.) were used to evaluate the agreement between Hybrid Capture 2 (hc2; Qiagen) and cobas 4800 (c4800; Roche Molecular Diagnostics) for the detection of high-risk human papillomavirus (HR HPV) genotype infections. The agreement between the two assays was 93.8% (kappa ‫؍‬ 0.87; 95% confidence interval, 0.828 to 0.918), with 186 and 251 concordant positive and negative results, respectively. All 186 concordant positives were confirmed using the Linear Array (LA; Roche Molecular Diagnostics) genotyping test. Of the 29 samples with discordant results (6.2%), 18 were hc2 positive and LA verified 17 as positive for HR HPV. Eleven discordant specimens were c4800 positive, and LA confirmed 5 as positive for HR HPV. As of October 2009, practice guidelines in Alberta, Canada, recommend reflex HPV testing for women over 30 years old with atypical squamous cells of undetermined significance (ASCUS) and for women over 50 years old with low-grade squamous intraepithelial lesions (LSIL) to help prioritize those who should undergo further evaluation. In this study, agreement between hc2 and c4800 results for samples from women over 30 years old with ASCUS cytology was 92.3% (n ‫؍‬ 13), while no samples from women over 50 years old with LSIL cytology were identified for analysis. Studies have shown that cervical cancer is a preventable disease, and its incidence and associated mortality rate have declined since the advent of Papanicolau (Pap) test screening programs (13,17). Opportunities for primary prevention and enhanced secondary prevention exist because of the understanding that human papillomaviruses (HPV) cause cervical cancer (33). To date, about 120 HPV genotypes have been classified (2); approximately 60 genotypes are known to infect the human genital tract, of which 12 genotypes (HPV 16,18,31,33, 35, 39, 45, 51, 52, 56, 58, and 59) are considered oncogenic or high risk (HR) and 5 genotypes (HPV 26, 53, 66, 68, and 72) are considered possibly oncogenic as their significance in cervical cancer incidence is unclear (21, 26). Persistent HR HPV infection is a requirement for progression from squamous intraepithelial lesions (SIL) to cervical intraepithelial neoplasia (CIN) and finally cervical cancer (7,10,16,27,30). Studies worldwide have shown that HPV 16 is detected most often (50 to 60%) in cervical cancer cases, followed by HPV 18 (10 to 12%) and HPV 31 and HPV 45 (4 to 5% each) (3). In the vast majority of women, HPV infections occur at a young age shortly after sexual debut and are transient. Detection of HR HPV in women over the age of 30 years is an indication that persistent infection may be occurring (7), which is necessary for the development of cervical cancer.Pap testing has limitations such as interoperator variability and low sensitivity. High-risk HPV testing (when used as either primary screening or for reflex testing following a nonnegative cytology result) improves patient management by providing a more accurate risk as...

show abstract

Section: Discussionsupporting

confidence: 64%

Section: Methodsmentioning

confidence: 95%

mentioning

confidence: 99%

See 1 more Smart Citation

Comparison of the Hybrid Capture 2 and cobas 4800 Tests for Detection of High-Risk Human Papillomavirus in Specimens Collected in PreservCyt Medium

et al. 2012

View full text Add to dashboard Cite

show abstract

“…The universal detection of HPV DNA usually focuses on the L1 region of the major capsid gene (Gravitt et al, 2000). Detection of the HPV DNA can be done by various techniques such as polymerase chain reaction (PCR) with specific primers, hybrid capture test, and linear array (De Antonio et al, 2008). Recently, a new technique to detect HPV DNA uses electrochemical DNA chip system combined with loop-mediated isothermal amplification (LAMP).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Detection Sensitivity for Human Papillomavirus between Self-collected Vaginal Swabs and Physician-collected Cervical Swabs by Electrochemical DNA Chip

Nilyanimit¹,

Wanlapakorn²,

Niruthisard³

et al. 2015

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Background: Human papillomavirus (HPV) DNA testing is an effective method to screen for precancerous changes in the cervix. Samples from self-collection rather than Pap smear can potentially be used to test for HPV as they are more acceptable and preferred for use in certain settings. The objective of this study was to compare HPV DNA testing from self-collected vaginal swabs and physician-collected cervical swabs. Materials and Methods: A total of 101 self-collected vaginal and physician-collected cervical swabs of known cytology from Thai women were tested by electrochemical DNA chip assay. The specimens were divided into 4 groups: 29 with normal cytology, 14 with atypical squamous cells of undetermined significance (ASCUS), 48 with lowgrade squamous intraepithelial lesion (LSIL), and 10 with high-grade squamous intraepithelial lesion (HSIL). Results: Positive detection rates of HPV from self-collected swabs were similar to those from physician-collected swabs. Among specimens with abnormal cytology, HPV was found in 50% of self-collected swabs and 47.2% of physician-collected swabs. In specimens with normal cytology, 17.2% of self-collected swabs and 24.1% of physician-collected swabs were positive for HPV. Concordance was relatively high between results from selfcollected and physician-collected samples. The most common HPV genotype detected was HPV 51. Conclusions: HPV DNA testing using self-collected swabs is a feasible alternative to encourage and increase screening for cervical cancer in a population who might otherwise avoid this important preventive examination due to embarrassment, discomfort, and anxiety.

show abstract

“…The detection of HPV DNA usually focuses on the L1 region of the major capsid-forming gene (Gravitt et al, 2000), and various techniques can be used to detect it such as polymerase chain reaction (PCR) with specific primers, Hybrid capture2 test (HCII) and linear array (De Antonio et al, 2008). Recently, a new technique for HPV DNA detection using an electrochemical DNA chip system combined with loop-mediated isothermal amplification (LAMP) was introduced.…”

Section: Introductionmentioning

confidence: 99%

Detection of Human Papillomavirus in Male and Female Urine by Electrochemical DNA Chip and PCR Sequencing

Nilyanimit

Wanlapakorn²,

Niruthisard³

et al. 2013

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Background: Cervical cancer is the second most common cancer in Thai women after breast cancer. Currently, the Papanicolaou (Pap) smear is the recommended procedure for cervical cancer screening in Thailand, but only a relatively small percentage of women follow this screening program. An alternative method to detect HPV genotypes associated with cervical cancer is self-sampling of urine, which is a more widely accepted method. Our study aimed to evaluate the prevalence of HPV in Thai women using urine and cervical swabs and prevalence of HPV in Thai men using urine samples. Materials and Methods: Tumorigenic HPV detection was accomplished by electrochemical DNA chip and PCR/direct sequencing. In addition to HPV prevalence, we report the concordance between different methods and sample types. One-hundred and sixteen women and 100 men were recruited. Histological examination revealed normal cytology in 52 women, atypical squamous cells of undetermined significance (ASCUS) in 9, low-grade squamous intraepithelial lesions (LSIL) in 24, and high-grade squamous intraepithelial lesions (HSIL) in 31. One-hundred men were classified as heterosexuals (n=45) and homosexuals (n=55). Results: The most prevalent HPV genotype in our study was HPV16. The HPV detection rate was generally lower in urine samples compared with cervical samples. Overall, there was good agreement for the detection of carcinogenic HPV from female cervical samples between the DNA chip and PCR/ sequencing, with 88.8% total agreement and a kappa value of 0.76. In male urine samples, the level of agreement was higher in heterosexuals compared with homosexuals. Conclusions: Further improvement is required to increase an overall yield of HPV DNA detection in urine samples before clinical application of a urine-based HPV screening program. The electrochemical DNA chip test is a promising technique for carcinogenic HPV detection.

show abstract

Detection of high-risk human papillomavirus by two molecular techniques: Hybrid capture and linear array

Cited by 11 publications

References 10 publications

Comparison of the Hybrid Capture 2 and cobas 4800 Tests for Detection of High-Risk Human Papillomavirus in Specimens Collected in PreservCyt Medium

Comparison of the Hybrid Capture 2 and cobas 4800 Tests for Detection of High-Risk Human Papillomavirus in Specimens Collected in PreservCyt Medium

Comparison of Detection Sensitivity for Human Papillomavirus between Self-collected Vaginal Swabs and Physician-collected Cervical Swabs by Electrochemical DNA Chip

Detection of Human Papillomavirus in Male and Female Urine by Electrochemical DNA Chip and PCR Sequencing

Contact Info

Product

Resources

About