Detection of hog cholera virus antigens in experimentally-infected pigs using an antigen-capture ELISA

Shannon, Anthony; Morrissy, Chris; Mackintosh, Susan; Westbury, H. A.

doi:10.1016/0378-1135(93)90014-x

Cited by 33 publications

(20 citation statements)

References 18 publications

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“…The antigen capture-ELISA (AC-ELISA) used in this study was developed at CSIRO AAHL based on that described by Shannon et al (1993) with local modifications (Fuqing et al 2000). The test relies on the use of three monoclonal antibodies to infer the presence of CSF virus antigen: (1) pestivirus group reactive; (2) bovine viral diarrhoea virus-specific; and (3) pestivirus negative antibodies.…”

Section: Antigen Capture-elisa For Csf Virusmentioning

confidence: 99%

“…The test relies on the use of three monoclonal antibodies to infer the presence of CSF virus antigen: (1) pestivirus group reactive; (2) bovine viral diarrhoea virus-specific; and (3) pestivirus negative antibodies. The results of the test were expressed as a signal-to-noise (S/N) ratio, where an S/N ratio >2 was indicative of the presence of CSF virus antigen (Fuqing et al 2000;Shannon et al 1993).…”

Section: Antigen Capture-elisa For Csf Virusmentioning

confidence: 99%

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Development and evaluation of a rapid immunomagnetic bead assay for the detection of classical swine fever virus antigen

Conlan

Khounsy

Blacksell

et al. 2008

Trop Anim Health Prod

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Classical swine fever (CSF) is a highly contagious and severe viral disease of swine resulting in substantial production losses in different farming systems in many regions of the world.The accurate and rapid detection of CSF outbreaks is reliant on sensitive and specific laboratory testing and is a key component of disease control. Specific detection of CSF virus can be achieved by virus isolation in tissue culture, antigen capture or the detection of viral RNA using molecular techniques. In order to reduce the time taken to achieve a diagnostic result and simplify testing methods, an antigen capture ELISA using immunomagnetic beads (IMB) as the solid phase was developed and compared to a microplate-based antigen capture (AC)-ELISA. The IMB-ELISA has up to 64-fold greater analytical sensitivity than the AC-ELISA and initial estimates of diagnostic sensitivity and specificity are 100%. The IMB-ELISA has a highly robust, rapid and stable test format and is simpler to perform than the AC-ELISA. The IMB-ELISA has the added advantage that a result can be sensitively and specifically determined by eye, lending it to the possibility of adaptation to a near-to-field test with minimal equipment or expertise needed.

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Section: Antigen Capture-elisa For Csf Virusmentioning

confidence: 99%

Section: Antigen Capture-elisa For Csf Virusmentioning

confidence: 99%

Development and evaluation of a rapid immunomagnetic bead assay for the detection of classical swine fever virus antigen

Conlan

Khounsy

Blacksell

et al. 2008

Trop Anim Health Prod

View full text Add to dashboard Cite

show abstract

“…Current diagnostic methods, including detection of viral antigens in tonsils by using fluorescent antibody (18), antigen capture enzyme-linked immunosorbent assay (4,21), and detection of genomic RNA by reverse transcription-PCR (3,8,11,12,24), are relatively rapid diagnostic tests; however, these techniques require centralized laboratory facilities and clinical specimen submissions, which delay disease diagnosis, thus affecting the efficiency of emergency disease management measures.…”

mentioning

confidence: 99%

Diagnostic Evaluation of a Real-Time Reverse Transcriptase PCR Assay for Detection of Classical Swine Fever Virus

Risatti

Holinka

et al. 2005

J Clin Microbiol

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A fluorogenic-probe hydrolysis (TaqMan)-reverse transcriptase (RT) PCR for classical swine fever virus (CSFV) was evaluated for diagnostic sensitivity and specificity by using clinical samples obtained from the Dominican Republic, where the disease is enzootic. The sensitivity of this test, using nasal swab samples taken from both symptomatic and asymptomatic animals, exceeded the diagnostic sensitivity of virus isolation (100% versus 72.4%, respectively) with little loss of specificity (98.9% versus 100%, respectively). At the herd level, three of four infected farms were identified by virus isolation, while the CSFV real-time RT-PCR assay identified all four infected premises. This simple and accurate test permits rapid detection of CSFV in affected herds.

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“…Rapid and precise detection of CSFV is critical for disease containment. Current diagnostic methods, including detection of viral antigens in tonsils by using fluorescent antibodies (28) or antigen capture enzyme-linked immunosorbent assay (6,32) and detection of genomic RNA by reverse transcription-PCR (3,11,13,15,39), are relatively rapid diagnostic tests; however, these techniques require centralized laboratory facilities and clinical specimen submissions that might delay disease diagnosis, thus affecting the efficiency of emergency disease management measures.…”

mentioning

confidence: 99%

Rapid Detection of Classical Swine Fever Virus by a Portable Real-Time Reverse Transcriptase PCR Assay

et al. 2003

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A fluorogenic-probe hydrolysis (TaqMan)-reverse transcriptase PCR assay for classical swine fever virus (CSFV) was developed and evaluated in experimentally infected swine. The assay detected CSFV, representing different phylogenetic groupings, but did not amplify viral RNA from related pestiviruses. The assay met or exceeded the sensitivity (1 to 100 50% tissue culture infective doses per ml) of viral cultures of samples from experimentally infected animals. Viral RNA was detected in nasal and tonsil scraping samples 2 to 4 days prior to the onset of clinical disease. The assay can be performed in 2 h or less, thus providing a rapid method for the diagnosis of classical swine fever.

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Detection of hog cholera virus antigens in experimentally-infected pigs using an antigen-capture ELISA

Cited by 33 publications

References 18 publications

Development and evaluation of a rapid immunomagnetic bead assay for the detection of classical swine fever virus antigen

Development and evaluation of a rapid immunomagnetic bead assay for the detection of classical swine fever virus antigen

Diagnostic Evaluation of a Real-Time Reverse Transcriptase PCR Assay for Detection of Classical Swine Fever Virus

Rapid Detection of Classical Swine Fever Virus by a Portable Real-Time Reverse Transcriptase PCR Assay

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