Detection of hospital environmental contamination during SARS-CoV-2 Omicron predominance using a highly sensitive air sampling device

Tan, Kai Sen; Ang, Alicia; Tay, Douglas Jie Wen; Somani, Jyoti; Ng, Alexander Jet Yue; Li, Peng; Chu, Justin Jang Hann; Tambyah, Paul Anantharajah; Allen, David Michael

doi:10.3389/fpubh.2022.1067575

Cited by 4 publications

(1 citation statement)

References 58 publications

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“…In addition, infectious virus was isolated from different hospital surfaces 30 and air samples 31 . Likewise, SARS-CoV-1 and SARS-CoV-2 RNA could be detected in air and surface samples 32,33 . Experimentally, infectious MERS-CoV at 20°C and 40% relative humidity could be recovered from plastic and steel surfaces for up to 48 hours 4 , and using a similar setup, SARS-CoV-1 and SARS-CoV-2 likewise retained viability for 48 hours 5 .…”

Section: Discussionmentioning

confidence: 88%

Transmission dynamics of MERS-CoV in a transgenic human DPP4 mouse model

Doremalen,

Bushmaker,

Fischer

et al. 2023

Preprint

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Since 2002, three novel coronavirus outbreaks have occurred: severe acute respiratory syndrome coronavirus (SARS-CoV-1), Middle East respiratory syndrome coronavirus (MERS-CoV), and SARS-CoV-2. A better understanding of the transmission potential of coronaviruses will result in adequate infection control precautions and an early halt of transmission within the human population. Experiments on the stability of coronaviruses in the environment, as well as transmission models, are thus pertinent. Here, we show that transgenic mice expressing human DPP4 can be infected with MERS-CoV via the aerosol route. Exposure to 5×106TCID50and 5×104TCID50MERS-CoV per cage via fomites resulted in transmission in 15 out of 20 and 11 out of 18 animals, respectively. Exposure of sentinel mice to donor mice one day post inoculation with 105TCID50MERS-CoV resulted in transmission in 1 out of 38 mice via direct contact and 4 out of 54 mice via airborne contact. Exposure to donor mice inoculated with 104TCID50MERS-CoV resulted in transmission in 0 out of 20 pairs via direct contact and 0 out of 5 pairs via the airborne route. Our model shows limited transmission of MERS-CoV via the fomite, direct contact, and airborne routes. The hDPP4 mouse model will allow assessment of the ongoing evolution of MERS-CoV in the context of acquiring enhanced human-to-human transmission kinetics and will inform the development of other transmission models.

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Section: Discussionmentioning

confidence: 88%

Transmission dynamics of MERS-CoV in a transgenic human DPP4 mouse model

Doremalen,

Bushmaker,

Fischer

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Advancements in CRISPR-diagnostic techniques for rapid on-site monitoring of environmental virus

Deng,

Liao,

et al. 2024

TrAC Trends in Analytical Chemistry

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Transmission dynamics of MERS-CoV in a transgenic human DPP4 mouse model

van Doremalen,

Bushmaker,

Fischer

et al. 2024

npj Viruses

View full text Add to dashboard Cite

Since 2002, three novel coronavirus outbreaks have occurred: severe acute respiratory syndrome coronavirus (SARS-CoV-1), Middle East respiratory syndrome coronavirus (MERS-CoV), and SARS-CoV-2. A better understanding of the transmission potential of coronaviruses will result in adequate infection control precautions and an early halt of transmission within the human population. Experiments on the stability of coronaviruses in the environment, as well as transmission models, are thus pertinent.Here, we show that transgenic mice expressing human DPP4 can be infected with MERS-CoV via the aerosol route. Exposure to 5 × 106 TCID50 and 5 × 104 TCID50 MERS-CoV per cage via fomites resulted in transmission in 15 out of 20 and 11 out of 18 animals, respectively. Exposure of sentinel mice to donor mice one day post inoculation with 105 TCID50 MERS-CoV resulted in transmission in 1 out of 38 mice via direct contact and 4 out of 54 mice via airborne contact. Exposure to donor mice inoculated with 104 TCID50 MERS-CoV resulted in transmission in 0 out of 20 pairs via direct contact and 0 out of 5 pairs via the airborne route. Our model shows limited transmission of MERS-CoV via the fomite, direct contact, and airborne routes. The hDPP4 mouse model will allow assessment of the ongoing evolution of MERS-CoV in the context of acquiring enhanced human-to-human transmission kinetics and will inform the development of other transmission models.

show abstract

Detection of hospital environmental contamination during SARS-CoV-2 Omicron predominance using a highly sensitive air sampling device

Cited by 4 publications

References 58 publications

Transmission dynamics of MERS-CoV in a transgenic human DPP4 mouse model

Transmission dynamics of MERS-CoV in a transgenic human DPP4 mouse model

Advancements in CRISPR-diagnostic techniques for rapid on-site monitoring of environmental virus

Transmission dynamics of MERS-CoV in a transgenic human DPP4 mouse model

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