Detection of HPV-16 genome in human oral cancers and potentially malignant lesions from India

D'Costa, Jenice; Saranath, Dhananjaya; Dedhia, Pratiksha; Sanghvi, Vikram; Mehta, Ashok

doi:10.1016/s1368-8375(98)00028-1

Cited by 131 publications

(115 citation statements)

References 24 publications

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“…12 Ha et al 10 detected the presence of HPV 16 DNA using real-time quantitative PCR in only 1 of 102 (1%) cases of oral premalignant lesions. Using PCR, D'Costa et al 19 detected HPV-16 in 34% of potentially malignant lesions and 31% of normal mucosa while Bouda et al 20 demonstrated high-risk HPV in oral epithelial dysplasia in all the five cases studied. In general, in situ hybridization is a more specific test for detecting the presence of HPV on formalin-fixed material and PCR is overly sensitive.…”

Section: Discussionmentioning

confidence: 94%

Human papillomavirus-associated oral intraepithelial neoplasia

2013

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This study evaluated an unusual subset of oral epithelial dysplasia for the presence of transcriptionally active high-risk HPV subtypes and to further characterize the histological criteria for this condition. There were 20 cases diagnosed as epithelial dysplasia with marked apoptosis of the anterior oral cavity. Clinical and followup data were collected and histopathological features were documented. Immunoperoxidase studies were performed for p16 and in situ hybridization studies were performed for low-and high-risk HPV sub-types. Gender-and site-matched controls of conventional moderate-to-severe oral epithelial dysplasia were similarly evaluated using immunoperoxidase studies for p16 and in situ hybridization; the number of apoptotic cells for study and control cases was counted at two different tissue sites. There were 17 men and 3 women with a median age of 56 years. Seventeen lesions were described as white and five were described as rough or papillary. Thirteen were located on the lateral or ventral tongue, some extending onto the floor of the mouth. Epithelial hyperplasia with marked karyorrhexis and apoptosis were present in all the cases, along with features of conventional oral epithelial dysplasia. A statistically significant number of apoptotic cells were identified in the study cases when compared with controls (P40.0001). Twenty cases were positive for high-risk HPV by in situ hybridization and all 19 nineteen cases evaluated for p16 demonstrated overexpression. Two patients were diagnosed with squamous cell carcinomas and one patient developed recurrent disease. We report a subset of oral epithelial dysplasia that occurs mostly in adult men on the ventral or lateral tongue and is positive for high-risk HPV and for p16. We propose use of the term 'HPV-associated Oral Intraepithelial Neoplasia' to characterize these lesions of the oral cavity for consistency in nomenclature with HPV-associated lesions of the lower anogenital tract. One case recurred and one developed invasive cancer. Keywords: HPV apoptotic oral epithelial dysplasia; koilocytic dysplasiaIn the United States, smoking frequency has decreased by 16% and the overall number of cases of cancer of the oral cavity and pharynx has fallen by 18% since 1975. 1 However, rates of cancers of the oral tongue, base of tongue, and palatine tonsils have continued to rise by 2-4% among younger men. 2,3 This may, in part, be explained by the increased prevalence of a subset of oropharyngeal carcinoma associated with human papillomavirus (HPV), mostly HPV-16. 4-7 Importantly, p16 overexpression is noted in almost all HPV-associated head and neck cancers and indicates that the virus is transcriptionally active. Patients with HPV-positive, p16-positive oropharyngeal carcinomas are slightly younger than those with tumors not associated with HPV and show improved survival. 8,9 It is generally believed that HPV has only a minor role in squamous cell carcinoma of the anterior oral cavity, because it has been identified in only 2.9-5.9% of cases and the beha...

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Section: Discussionmentioning

confidence: 94%

Human papillomavirus-associated oral intraepithelial neoplasia

2013

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“…However, D'Costa et al [14] and Schwartz et al [17] found a higher frequency of HPV in tongue OSCC, whereas Premoli-De-Percoco and Ramirez [18] and Sugiyama et al [19] observed higher rates in cases in which the tumor involved the floor of the mouth and jugal mucosa.…”

Section: Discussionmentioning

confidence: 96%

Human papillomavirus in oral squamous cells carcinoma in a population of 75 Brazilian patients

Soares

Oliveira

Souza

et al. 2007

American Journal of Otolaryngology

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Purpose: In the present study, we investigated the presence of human papillomavirus (HPV) DNA and viral types in 75 cases of oral squamous cells carcinoma from Brazil to obtain data that would contribute to a better understanding of the role of HPV in the pathogenesis of this tumor. Materials and methods: DNA was extracted from paraffin-embedded tissue and amplified by polymerase chain reaction using a pair of primers designated PCO3+ and PCO4+ for the detection of a fragment of the human h-globin gene, followed by polymerase chain reaction for the detection of HPV DNA using a pair of generic primers, GP5+ and GP6+. Viral typing was performed by dot blot hybridization.Results: Human papillomavirus DNA was detected in 18 (24%) of the 75 cases positive for the human h-globin gene. No significant association was observed between HPV and age, sex, or anatomical location of the tumor. The most prevalent viral type was HPV-18 (77,8%). Conclusion:The low frequency of detection of HPV DNA in oral epidermoid carcinomas suggests a possible participation of the virus in the development and progression of only a subgroup of these tumors. D

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“…The reported HPV prevalence in oral cancers in India varied from 0 to 100% in studies using biopsies in cases and PCR assay for HPV detection and genotyping. [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] For the studies using a methodology similar to ours (oral brushing to collect epithelial cells instead of biopsy, combined with PCR for detection and genotyping), the prevalence reported for oral cancer cases was 31.5 26 and 32.4%. 15 Our results are in agreement with two studies conducted in Gujarat and Mumbai in West India, which reported a null prevalence of HPV in oral cancer using biopsies (in one of the studies, only HPV-16 and 18 were tested).…”

Section: Discussionmentioning

confidence: 99%

“…12 One-on-one semistructured interviews using a questionnaire and life grid technique, which has been shown to improve recall, 13 were conducted by three professionally trained dentists. Data collected included sociodemographic information, religious beliefs, environmental variables and sexual behavior including age at first sexual intercourse, number of sexual partners and oral sex practice frequency during three different life stages ( 16,(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) and >30 years).…”

Section: Patient and Data Collectionmentioning

confidence: 99%

No role for human papillomavirus infection in oral cancers in a region in southern India

Laprise

Madathil

Allison

et al. 2015

Intl Journal of Cancer

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Oral cancer is a major public health issue in India with~77,000 new cases and 52,000 deaths yearly. Paan chewing, tobacco and alcohol use are strong risk factors for this cancer in India. Human papillomaviruses (HPVs) are also related to a subset of head and neck cancers (HNCs). We examined the association between oral HPV and oral cancer in a sample of Indian subjects participating in a hospital-based case-control study. We recruited incident oral cancer cases (N 5 350) and controls frequencymatched by age and sex (N 5 371) from two main referral hospitals in Kerala, South India. Sociodemographic and behavioral data were collected by interviews. Epithelial cells were sampled using Oral CDx V R brushes from the oral cancer site and the normal mucosa. Detection and genotyping of 36 HPV genotypes were done using a polymerase chain reaction protocol. Data collection procedures were performed by qualified dentists via a detailed protocol with strict quality control, including independent HPV testing in India and Canada. HPV DNA was detected in none of the cases or controls. Associations between oral cancer and risk factors usually associated with HPV infection, such as oral sex and number of lifetime sexual partners, were examined by logistic regression and were not associated with oral cancer. Lack of a role for HPV infection in this study may reflect cultural or religious characteristics specific to this region in India that are not conducive to oral HPV transmission. A nationwide representative prevalence study is needed to investigate HPV prevalence variability among Indian regions.Worldwide, nearly 300,000 new cases of oral cancers are diagnosed each year, accounting for 145,000 deaths. Approximately 77,000 new cases and 52,000 oral cancer-related deaths occur yearly in India, representing a quarter of all incident cases in the world. 1 Known risk factors include tobacco smoking and chewing, high alcohol consumption and, in a country such as India, paan chewing. 2 Oral human papillomavirus (HPV) infection has recently been recognized as another important risk factor for head and neck cancer (HNC), mainly for the oropharyngeal cancer subsite. In fact,

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Detection of HPV-16 genome in human oral cancers and potentially malignant lesions from India

Cited by 131 publications

References 24 publications

Human papillomavirus-associated oral intraepithelial neoplasia

Human papillomavirus-associated oral intraepithelial neoplasia

Human papillomavirus in oral squamous cells carcinoma in a population of 75 Brazilian patients

No role for human papillomavirus infection in oral cancers in a region in southern India

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