Detection of Human Bocavirus in Japanese Children with Lower Respiratory Tract Infections

Ma, Xiaoming; Endo, Rika; Ishiguro, Nobuhisa; Ebihara, Takashi; Ishiko, Hiroaki; Ariga, Tadashi; Kikuta, Hídeaki

doi:10.1128/jcm.44.3.1132-1134.2006

Cited by 205 publications

(252 citation statements)

References 7 publications

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“…HBoV ile enfekte olgularımızın laboratuvar bulguları değerlendirildiğinde, lökosit sayısı ve CRP değerlerinde artış olduğu izlenmiş, ancak bu fark istatistiksel olarak anlamlı bulunmamıştır (Tablo IV). Bu bulgu, diğer araştırmacıların verileri ile uyumludur 34,36,40 . Yapılan çalışmalarda HBoV enfeksiyonlarında patolojik akciğer grafisi bulgusu oranları %43-83 arasında değişmektedir 36,41 .…”

Section: Discussionunclassified

Determination of the Frequency of Human Bocavirus and Other Respiratory Viruses Among 0-2 Years Age Group Children Diagnosed as Acute Bronchiolitis

Uyar¹,

Kuyucu²,

Tezcan³

et al. 2014

Mikrobiyol Bul

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* Bu çalışma, Mersin Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir (Proje no. TF DTB [MU] 2010-4 TU). ÖZETAkut bronşiyolit, daha çok iki yaşından küçük çocuklarda sıklıkla viral etkenlerin neden olduğu bir alt solunum yolu enfeksiyonudur. Bu çalışmada, 0-2 yaş arası akut bronşiyolitli çocuklarda, insan bokavirusu (HBoV) da dahil olmak üzere solunum yolu viruslarının sıklığının belirlenmesi ve bu etkenlerle oluşan enfeksiyonların klinik özelliklerinin değerlendirilmesi amaçlanmıştır. Çalışmaya, Ocak-Haziran 2010 tarihleri arasında Mersin Üniversitesi Tıp Fakültesi, Çocuk Sağlığı ve Hastalıkları Anabilim Dalında akut bronşiyolit tanısı alan 62 hasta (45 erkek, 17 kız; yaş aralığı: 0-2 yıl) ile kontrol grubu olarak sağlıklı 33 çocuk (21 erkek, 12 kız; yaş aralığı: 0-2 yıl) dahil edilmiştir. Çalışma gruplarından alınan nazofarengeal aspirat örneklerinde solunum yolu virusları [respiratuar sinsityal virusu (RSV) A ve B; rhinovirus (RV); insan metapnömovirusu (hMPV) A ve B; influenza virus tip A (H1N1, H3N2, H1N1v), B ve C; parainfluenza virus (PIV) tip 1, 2, 3 ve 4A/B; adenovirus (AdV); HBoV; koronavirus (CoV 229E); enterovirus (EV)], multipleks polimeraz zincir reaksiyonu (M-PCR) ve DNA mikroarray prensibi ile çalışan CLART ® Pneumovir (Clinical Array Technology, Genomica, İspanya) ticari sistemi ile araştırılmıştır. Ayrıca, viral etkenler ile hastaların demografik, klinik ve laboratuvar özellikleri, uygulanan tedaviler ve hastanede yatış oranları arasındaki ilişki değerlendirilmiştir. Çalışmamızda, akut bronşiyolitli 62 hastanın 52 (%83.9)'sinde en az Geliş Tarihi (Received): 12.12.2013 • Kabul Ediliş Tarihi (Accepted): 11.04.2014

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Section: Discussionunclassified

Determination of the Frequency of Human Bocavirus and Other Respiratory Viruses Among 0-2 Years Age Group Children Diagnosed as Acute Bronchiolitis

Uyar¹,

Kuyucu²,

Tezcan³

et al. 2014

Mikrobiyol Bul

View full text Add to dashboard Cite

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“…Although IgG antibody present in a single serum sample does not reflect an acute infection, a high rate of IgG positive sera from healthy children does suggest that HBoV infection is more common in children than genomic detection has shown previously (Allander et al, 2005;Arnold et al, 2006;Bastien et al, 2006;Choi et al, 2006;Foulongne et al, 2006;Lin et al, 2007;Ma et al, 2006;Qu et al, 2007;Sloots et al, 2006;Weissbrich et al, 2006). Infection with B19 occurs in children in a high incidence asymptomatically.…”

Section: Discussionmentioning

confidence: 99%

“…The incidence of HBoV has been reported to be between 1.5% to 11.3% based on tests of respiratory samples from individuals with acute respiratory illness (Allander et al, 2005;Arnold et al, 2006;Bastien et al, 2006;Choi et al, 2006;Foulongne et al, 2006;Lin et al, 2007;Ma et al, 2006;Qu et al, 2007;Sloots et al, 2006;Weissbrich et al, 2006). HBoV appears to be associated with lower respiratory tract infections, and in many cases, co-infection with other respiratory viruses (Allander et al, 2007;Fry et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

ELISAs using human bocavirus VP2 virus-like particles for detection of antibodies against HBoV

Lin

Guan

Cheng

et al. 2008

Journal of Virological Methods

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Human bocavirus (HBoV) has been identified worldwide in children with lower respiratory tract infections with an incidence of approximately 2% −11%. The role of HBoV in pathogenesis, however, is largely unknown, and little is known about the epidemiology of the virus. To study the seroepidemiology of HBoV infection, the capsid protein was expressed in insect cells. Expression of the putative major capsid protein VP2 in insect cells led to the formation of virus-like particles exhibiting the typical icosahedral appearance of parvoviruses with a diameter of approximately 20 nm. The expressed particles were used to establish an ELISA method, and serum samples from groups of children of various ages in China were tested for IgG antibodies against HBoV. HBoV antibodies were detected in as high as 36% of healthy children under 9 years. Of children hospitalized with lower respiratory tract infections, 31% were seropositive, and all age groups of these children showed a significantly higher level of HBoV IgG antibody than their healthy counterparts. When divided into age cohorts, results showed that more than 48% of healthy children had seroconverted by age of 4. Thus, HBoV appears to be a common infection in children. The potential pathogenesis of this virus, especially its role in lower respiratory tract infections in children warrants further investigation.

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“…2,3). Most prevalence studies were done on samples from children with respiratory tract diseases, with rates observed between 1.5-19% (1,(4)(5)(6)(7)(8)(9)(10)(11)(12). Studies made on fecal specimens indicate that HBoV is also prevalent in children with acute gastroenteritis, with 0.8-16% of samples being HBoV + (4,(13)(14)(15).…”

mentioning

confidence: 99%

Human Bocavirus NP1 Inhibits IFN-β Production by Blocking Association of IFN Regulatory Factor 3 with IFNB Promoter

Zhang

Zheng

Luo

et al. 2012

The Journal of Immunology

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Human bocavirus (HBoV) mainly infects young children. Although many infected children suffer from respiratory or gastroenteric tract diseases, an association between HBoV and these diseases is not definite. Because modulation of type I IFN is crucial for viruses to establish efficient replication, in this study, we tested whether HBoV modulates type I IFN production. We observed that a nearly full-length HBoV clone significantly reduced both Sendai virus (SeV)- and poly(deoxyadenylic-thymidylic) acid-induced IFN-β production. Further study showed that NP1 blocked IFN-β activation in response to SeV, poly(deoxyadenylic-thymidylic) acid, and IFN-β pathway inducers, including retinoic acid-inducible protein I, mitochondrial antiviral signaling protein, inhibitor of κB kinase ε, and TANK-binding kinase 1. In addition, NP1 interfered with IRF-3–responsive PRD(III-I) promoter activated by SeV and a constitutively active mutant of IRF-3 (IRF-3/5D). Although NP1 suppressed the IRF-3 pathway, it did not affect IRF-3 activation processes, including phosphorylation, dimerization, and nuclear translocation. Coimmunoprecipitation assays confirmed the interaction between NP1 and IRF-3. Additional deletion mutagenesis and coimmunoprecipitation assays revealed that NP1 bound to the DNA-binding domain of IRF-3, resulting in the interruption of an association between IRF-3 and IFNB promoter. Altogether, our results indicate that HBoV NP1 blocks IFN production through a unique mechanism. To our knowledge, this is the first study to investigate the modulation of innate immunity by HBoV. Our findings suggest a potential immune-evasion mechanism used by HBoV and provide a basis for better understanding HBoV pathogenesis.

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Detection of Human Bocavirus in Japanese Children with Lower Respiratory Tract Infections

Cited by 205 publications

References 7 publications

Determination of the Frequency of Human Bocavirus and Other Respiratory Viruses Among 0-2 Years Age Group Children Diagnosed as Acute Bronchiolitis

Determination of the Frequency of Human Bocavirus and Other Respiratory Viruses Among 0-2 Years Age Group Children Diagnosed as Acute Bronchiolitis

ELISAs using human bocavirus VP2 virus-like particles for detection of antibodies against HBoV

Human Bocavirus NP1 Inhibits IFN-β Production by Blocking Association of IFN Regulatory Factor 3 with IFNB Promoter

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