Detection of human cytomegalovirus and Epstein-Barr Virus in symptomatic and asymptomatic apical periodontitis lesions by real-time PCR

Abstract

Objectives: Recent studies have investigated the occurrence of human cytomegalovirus and Epstein-Barr Virus in samples from apical periodontitis lesions and a role in the pathogenesis of this disease has been suggested. Because genotype distribution and seroprevalence of EBV and HCMV differ among populations, it is important to determine the presence of these viruses in endodontic periapical lesions of different populations. The aims of this study were to determine the presence of HCMV and EBV DNAs in samples from Turkish patients with symptomatic and asymptomatic apical periodontitis lesions using real-time polymerase chain reaction method and to evaluate their presence in both symptomatic and asymptomatic apical periodontitis lesions. Study Design: Periapical samples were collected from 12 asymptomatic and 16 symptomatic periapical lesions in conjunction with apicectomy. HCMV and EBV DNAs were identified in the samples by real-time PCR. The chi-squared test with Yates’s correction or the Fisher’s exact test was used to analyse the significance of differences. Results: HCMV DNA was detected in 10 of the 16 (62.5%) symptomatic and in five of the 12 (41.7 %) asymptomatic periapical study lesions. The EBV DNA was identified in seven of the 16 (43.7 %) symptomatic and three of the 12 (25 %) asymptomatic periapical lesions. The difference in occurrence of HCMV and EBV DNA between symptomatic and asymptomatic periapical lesions was not statistically significant. (All comparisons have p > 0.05). Conclusions: Our findings suggest that HCMV and EBV is a frequent inhabitant of both symptomatic and asymptomatic apical periodontitis lesions of endodontic origin in Turkish population. Key words:Human cytomegalovirus, Epstein-Barr Virus, apical periodontitis, Polymerase chain reaction method.

Herpesviral and “Red Complex” Bacterial Analysis of Acute Apical Abscesses

“…They found that human herpesvirus-8 and human papillomavirus were the most prevalent viruses showing associations with the target bacterial species. The lower prevalence of viral-bacterial associations in our study may have resulted from the clinical status of the study subjects, viral diagnostic methods used, or geographic differences in herpes viral occurrence [3,8,11,19,24].…”

“…uli. The differences might occur for several reasons, such as differences in the primers used and methods of DNA extraction, differences in the endodontic microbiota, and several host and environmental factors, such as genetic background, ethnicity, socioeconomic status, psychologic stress, smoking, and the nature of the species colonizing other individuals in the same country [11,24,25].…”

Viruses in pulp and periapical inflammation: a review

“…It is important to do differential diagnosis in these cases, to avoid making misdiagnoses resulting in unnecessary dental treatment. EBV was the virus most associated with endodontic [2,25,26,[28][29][30][31][34][35][36][37][38][39][40][41][42][43]. In all these studies, the EBV was present in the samples, with the exception of Rosaline's study [35].…”

“…The main association between viruses and endodontic diseases, that was HCMV and EBV, but still remains to be determined whether these associations have an effect on the symptoms or radiographic characteristics of apical lesions, or only have a minor clinical finding [2,25,26,29,30,34,36,37,[39][40][41][42][43].…”

The sero-epidemiology of infectious mononucleosis in Neyshabur, Northeast Iran during 2010–2014

“…Some individuals are more vulnerable than others to develop clinical symptoms from delayed infection, while there is no reason [5,6]. CD8 + T cells play a key protective role for the control of latent EBV infection.…”