Detection of human enterovirus and human parechovirus (HPeV) genotypes from clinical stool samples: polymerase chain reaction and direct molecular typing, culture characteristics, and serotyping

Benschop, Kimberley; Minnaar, R. P.; Koen, Gerrit; Eijk, Hetty van; Dijkman, Karen; Westerhuis, Brenda M.; Molenkamp, Richard; Wolthers, Katja C.

doi:10.1016/j.diagmicrobio.2010.05.016

Cited by 92 publications

(120 citation statements)

References 34 publications

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“…HPeV4 is frequently found in stools as well (Benschop et al, 2008c(Benschop et al, , 2010aBoros et al, 2010;Pham et al, 2011b;Zhong et al, 2011), while HPeV6 seems to prevail as a secondary respiratory pathogen (Harvala et al, 2008). Infections with HPeV2 and 5 are reported sporadically (Benschop et al, 2010a;Ehrnst & Eriksson, 1996;van der Sanden et al, 2008). Circulation patterns of the newly reported HPeV types 7-16 are yet to be determined (Benschop et al, 2008c(Benschop et al, , 2010avan der Sanden et al, 2008).…”

Section: Epidemiology Of Hpev In Relation To Cns Infectionsmentioning

confidence: 99%

“…Similar data were found in studies on stool samples in other European countries (Baumgarte et al, 2008;Tapia et al, 2008), and Asia (Ito et al, 2010;Pham et al, 2010Pham et al, , 2011aWatanabe et al, 2007) where HPeV1 was identified as the most prevalent type, followed by HPeV3. HPeV4 is frequently found in stools as well (Benschop et al, 2008c(Benschop et al, , 2010aBoros et al, 2010;Pham et al, 2011b;Zhong et al, 2011), while HPeV6 seems to prevail as a secondary respiratory pathogen (Harvala et al, 2008). Infections with HPeV2 and 5 are reported sporadically (Benschop et al, 2010a;Ehrnst & Eriksson, 1996;van der Sanden et al, 2008).…”

Section: Epidemiology Of Hpev In Relation To Cns Infectionsmentioning

confidence: 99%

“…Epidemiological studies dating back over 30 year ago, showed HPeV1 to be widely spread (Ehrnst & Eriksson, 1993;Grist et al, 1978;Joki-Korpela & Hyypia, 1998;Khetsuriani et al, 2006b). In recent studies, involving the detection of the newer HPeV types, the new HPeV type 3 is identified as the second predominant strain (Benschop et al, 2006a(Benschop et al, , 2008c(Benschop et al, , 2010aIto et al, 2010;Tapia et al, 2008;Watanabe et al, 2007). HPeV3 comprised 22 to 26% of the HPeV types identified in HPeV positive stool samples collected in the Amsterdam region, while the majority of the HPeV positive stool samples comprised HPeV1 (65-71%) (Benschop et al, 2006a(Benschop et al, , 2008c(Benschop et al, , 2010a.…”

Section: Epidemiology Of Hpev In Relation To Cns Infectionsmentioning

confidence: 99%

“…HPeV1 is considered as the predominant strain mainly affecting young children (Baumgarte et al, 2008;Benschop et al, 2006aBenschop et al, , 2008cBenschop et al, , 2010aHarvala et al, 2008;Joki-Korpela & Hyypia, 1998;Stanway et al, 2000;Tapia et al, 2008;Watanabe et al, 2007). Epidemiological studies dating back over 30 year ago, showed HPeV1 to be widely spread (Ehrnst & Eriksson, 1993;Grist et al, 1978;Joki-Korpela & Hyypia, 1998;Khetsuriani et al, 2006b).…”

Section: Epidemiology Of Hpev In Relation To Cns Infectionsmentioning

confidence: 99%

“…In recent studies, involving the detection of the newer HPeV types, the new HPeV type 3 is identified as the second predominant strain (Benschop et al, 2006a(Benschop et al, , 2008c(Benschop et al, , 2010aIto et al, 2010;Tapia et al, 2008;Watanabe et al, 2007). HPeV3 comprised 22 to 26% of the HPeV types identified in HPeV positive stool samples collected in the Amsterdam region, while the majority of the HPeV positive stool samples comprised HPeV1 (65-71%) (Benschop et al, 2006a(Benschop et al, , 2008c(Benschop et al, , 2010a. Similar data were found in studies on stool samples in other European countries (Baumgarte et al, 2008;Tapia et al, 2008), and Asia (Ito et al, 2010;Pham et al, 2010Pham et al, , 2011aWatanabe et al, 2007) where HPeV1 was identified as the most prevalent type, followed by HPeV3.…”

Section: Epidemiology Of Hpev In Relation To Cns Infectionsmentioning

confidence: 99%

See 4 more Smart Citations

Human Parechoviruses, New Players in the Pathogenesis of Viral Meningitis

Benschop

Wildenbeest²,

Wolthers³

2012

Meningitis

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Section: Epidemiology Of Hpev In Relation To Cns Infectionsmentioning

confidence: 99%

Section: Epidemiology Of Hpev In Relation To Cns Infectionsmentioning

confidence: 99%

Section: Epidemiology Of Hpev In Relation To Cns Infectionsmentioning

confidence: 99%

Section: Epidemiology Of Hpev In Relation To Cns Infectionsmentioning

confidence: 99%

Section: Epidemiology Of Hpev In Relation To Cns Infectionsmentioning

confidence: 99%

See 3 more Smart Citations

Human Parechoviruses, New Players in the Pathogenesis of Viral Meningitis

Benschop

Wildenbeest²,

Wolthers³

2012

Meningitis

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Human Parechoviruses: Biology and Infection

Fischer

Harvala

Midgley

2017

Encyclopedia of Life Sciences

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Infections with human parechoviruses (HPeVs) are highly prevalent, particularly in neonates, where they may cause substantial morbidity and mortality. The clinical presentation of HPeV infection in neonates is indistinguishable from that of enterovirus (EV) infection and may vary from mild disease involving gastrointestinal and/or respiratory symptoms to more severe illness with sepsis‐like symptoms such as fever, seizures, irritability, rash and/or feeding problems. HPeV is among the most common single causes of aseptic meningitis/meningoencephalitis in infants <90 days old worldwide. The mainstay of diagnosis for HPeV infections involves the use of molecular diagnostic techniques for detection of virus RNA (ribonucleic acid) in various materials (e.g. faeces, cerebrospinal fluid (CSF), nasopharyngeal, bronchial and autopsy materials). Despite the relatedness of HPeV and EV both at the genomic and clinical presentation levels, routine nucleic acid detection methods for EV will not detect HPeV. Laboratory diagnosis of these viral infections is important not only for differential diagnostic purposes and determining a patient's prognosis but also for guidance of clinical management. Clinical disease varies somewhat among genotypes; while HPeV1 mostly causes gastrointestinal illness, HPeV3 prominence is due to sepsis syndromes and central nervous system (CNS) infections in preterm and term infants. Of diagnostic importance, HPeV3 CNS infections usually lack CSF pleocytosis, and despite ‘unremarkable’ CSF, HPeV3 RNA can be amplified from CSF, nasopharyngeal and rectal swabs in an infected individual. Taking the global incidence of reported laboratory‐confirmed HPeV cases into consideration, mortality and sequelae are overall uncommon and usually accompanying initially severe or neurologically complicated acute illnesses. However, underreporting due to lack of testing and/or appropriate diagnostic methodology likely means gross underestimation of the true incidence of HPeV disease. Currently, neither effective treatment nor vaccines are available in the control of these common viruses with the potential to cause severe harm in neonates and young children worldwide. Key Concepts HPeV infections are common among infants and children worldwide. HPeV infections are underdiagnosed, and HPeV disease burden underestimated. Nineteen HPeV types identified so far – their role/significance in human infections unclear except for HPeV3. HPeV3 is a major cause of neonatal infections including severe meningoencephalitis. Genomic differences between EV and HPeV. Diagnostics: HPeV required specific PCR for detection, not that easy to culture. Majority of children with HPeV3 infection lack pleocytosis in CSF/have normal CSF. Having older siblings are associated with higher risk for an infant to be hospitalised with severe HPeV infection. HPeV infection should always be ruled out when caring for infants with sepsis‐like illness and/or CNS infection.

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Enteroviruses

Parikh,

Eberly

2024

Manual of Molecular and Clinical Laboratory Immunology

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Detection of human enterovirus and human parechovirus (HPeV) genotypes from clinical stool samples: polymerase chain reaction and direct molecular typing, culture characteristics, and serotyping

Cited by 92 publications

References 34 publications

Human Parechoviruses, New Players in the Pathogenesis of Viral Meningitis

Human Parechoviruses, New Players in the Pathogenesis of Viral Meningitis

Human Parechoviruses: Biology and Infection

Enteroviruses

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