Detection of human papilloma virus (HPV) and K-ras mutations in human lung carcinomas

Noutsou, A.; Koffa, Maria; Ergazaki, M.; Siafakas, Nikos M.; Spandidos, Demetrios Α.

doi:10.3892/ijo.8.6.1089

Cited by 13 publications

(12 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…But in our study, no difference was found in gender and age. It was similar to the report from Noutsou et al (28) that no correlation was found between HPV infection with sex, age and clinical outcome of the patient, or the histological type and the differentiation grade of the tumor.…”

Section: Discussionsupporting

confidence: 91%

Correlation of HPV-16/18 infection of human papillomavirus with lung squamous cell carcinomas in Western China

Yan

2009

Oncol Rep

View full text Add to dashboard Cite

Abstract. Prevalent inconsistency of human papillomavirus (HPV) infection in lung cancer was found among recent studies from different countries with racial and geographic variations. Even in Chinese populations, the prevalent discrepancy of HPV infection in lung cancer patients was also found with the geographic variations and tumor types. To study the difference of HPV-16/18 infection in lung squamous cell carcinomas and non-cancer controls, we conducted this study to verify whether there was a similar HPV infection prevalence pattern in lung squamous cell carcinoma patients from the Western part of China. Paraffinembedded samples (180), of 72 lung squamous cell carcinomas, 37 lung adenocarcinomas and 71 non-cancer controls, were analyzed by INNO-LIPA Genotype polymerase chain reaction (PCR) and real-time PCR analysis. The data showed that 51.4% (37/72) of lung squamous cell carcinoma samples, 16.2% (6/37) of adenocarcinoma, and 22.5% (16/71) of non-cancer controls were HPV DNA positive. The risk of lung squamous cell carcinomas was 3.5 times higher among people HPV-positive (odds ratio 3.5, 95% CI 1.6-7.3, p<0.001) compared with the HPV-negative population. Adjusted by smoking status, the risk of lung squamous cell carcinomas was 3.5 times higher among people HPVpositive (odds ratio 3.5, 95% CI 1.7-7.5, p=0.001) compared with the HPV-negative population. The risk of lung squamous cell carcinomas was 16.9 times higher for patients with positive HPV-16 (odds ratio 16.9, 95% CI 3.8-75.3, p<0.0001) than negative HPV-16. Adjusted by smoking status, the risk of lung squamous cell carcinomas was 17.4 times higher among people HPV-16 positive (odds ratio 17.4, 95% CI 3.9-77.5, p<0.0001) compared with HPV-16 negative people. INNO-LIPA Genotype analysis revealed that the frequency of high risk HPV-16 and 18 genotype in lung squamous cell carcinoma cases appeared to be significant higher than that in the non-cancer controls (P<0.001), and the most frequent genotype was HPV-16 (21 cases), followed by HPV-18 (6 cases), HPV-16/18 (4 cases) in lung squamous cell carcinomas, respectively, and the majority genotype was HPV-6 (9 cases) in controls. The HPV-16 E 2 /E 6 ratio was significantly lower than the unity, suggested that the main presence of integrated form of HPV-16 genome (16/27) may contribute more to lung squamous cell carcinomas, despite its viral load estimated to be only <1 to 2 copies per cell.

show abstract

Section: Discussionsupporting

confidence: 91%

Correlation of HPV-16/18 infection of human papillomavirus with lung squamous cell carcinomas in Western China

Yan

2009

Oncol Rep

View full text Add to dashboard Cite

show abstract

“…In our series, HPV16 has been found in three large cell carcinomas and one poorly differentiated squamous cell carcinoma. The presence of HPV in large cell carcinomas is unusual since it has been reported in only two studies 24,25 with the presence of HPV16 in one case and of HPV31-33 in two cases. Of particular interest is the absence of koilocytes in all our positive cases.…”

Section: Discussionmentioning

confidence: 92%

Prevalence of human papillomaviruses in lung carcinomas: a study of 218 cases

et al. 2005

View full text Add to dashboard Cite

High-risk human papillomaviruses (HPV) are largely implicated in the carcinogenesis of cervical carcinomas. Their role in bronchopulmonary carcinomas is still unclear. In the present study, we have explored 218 fresh frozen lung tumours for the presence of HPV with the Roche line blot assay and for the expression of mRNAs encoding E6 oncoprotein in HPV positive tumours. Only four samples were positive for HPV detection, one poorly differentiated squamous cell carcinoma and three large cell carcinomas. E6 mRNA was undetectable in these four samples. Our data confirm the low prevalence of HPV in lung carcinomas in Western European countries and do not plead in favour of a carcinogenic role for HPV in these carcinomas.

show abstract

“…Since Noutsou et al (36) pointed out the involvement of highrisk HPVs in the carcinogenesis of the lung in cooperation with K-Ras mutation, it is worthwhile to examine the frequency of K-Ras mutation in HPV-positive and -negative lung carcinomas.…”

Section: ------------------------------------------------------------mentioning

confidence: 99%

Human papillomavirus is frequently detected in gefitinib-responsive lung adenocarcinomas

Baba

Castillo²,

Koriyama³

et al. 2010

Oncol Rep

View full text Add to dashboard Cite

Abstract.A number of studies have reported the presence of human papillomavirus (HPV) in lung carcinoma. Interestingly, its detection rate appears to differ histologically and geographically. The present study examined 30 adenocarcinomas and 27 squamous cell carcinomas of the lung in a southern area of Japan, and detected high-risk HPV genome in 9 (30%) adenocarcinomas and 2 (7%) squamous cell carcinomas, using PCR with SPF10 primers and INNO-LiPA HPV genotyping assay. The difference of HPV detection rates in adenocarcinomas and squamous cell carcinomas was statistically significant (P=0.044, Fisher's exact test). HPV-16 was the most prevalent HPV genotype, and was detected in 27% (8/30) of adenocarcinomas and in 7% (2/27) of squamous cell carcinomas. High-risk-HPV positive carcinomas had decreased proportions of pRb (P=0.107) and significantly increased proportions of p16INK4a expressing cells (P=0.031) when compared to HPV-negative lung carcinomas. All HPV-16-positive cases were considered to have an integrated form of HPV-16 but its viral load was low (geometric mean = 0.02 copy per cell). In 20 additional adenocarcinomas treated with gefitinib, a tyrosine kinase inhibitor specific for epidermal growth factor receptor, the presence of HPV was examined. Note that East Asian ethnicity is a predictive factor of gefitinib response. High-risk HPV genome was found in 75% (6/8) of adenocarcinomas with complete or partial response to gefitinib but was not found in the remaining 12, which did not respond to gefitinib. In conclusion, the present study suggests that high-risk HPV may be more strongly related to adenocarcinomas, particularly gefitinib-responsive adenocarcinomas, when compared to squamous cell carcinomas. However, its low viral load makes it difficult to determine the etiological significance of these findings.

show abstract

Detection of human papilloma virus (HPV) and K-ras mutations in human lung carcinomas

Cited by 13 publications

References 31 publications

Correlation of HPV-16/18 infection of human papillomavirus with lung squamous cell carcinomas in Western China

Correlation of HPV-16/18 infection of human papillomavirus with lung squamous cell carcinomas in Western China

Prevalence of human papillomaviruses in lung carcinomas: a study of 218 cases

Human papillomavirus is frequently detected in gefitinib-responsive lung adenocarcinomas

Contact Info

Product

Resources

About