Detection of human papillomavirus in oropharyngeal squamous cell carcinoma

Fauzi, Fatin Hazwani; Hamzan, Nurul Izzati; Rahman, Nurhayu Ab; Suraiya, Siti; Mohamad, Suharni

doi:10.1631/jzus.b2000161

Cited by 18 publications

(11 citation statements)

References 83 publications

(109 reference statements)

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“…It has been reported that RNA ISH technique had a higher sensitivity, specificity and positive and negative predictive values in comparison to p16, and HPV DNA ISH techniques in identifying HR-HPV (26). Although, sensitivity of HPV DNA ISH is a bit lower in HPV related cancer types in different studies (63%-67%) (41,42), it showed 91.3% sensitivity for the detection of HPV in HPVA tumors in our series. This result has been increased to 97.9% (92/94) by combining HPV DNA ISH and p16 IHC results.…”

Section: Discussioncontrasting

confidence: 55%

Reproducibility of Morphologic Parameters of the International Endocervical Adenocarcinoma Criteria and Classification System and Correlation With Clinicopathologic Parameters: A Multi-Institutional Study

Bulutay

Haberal

Özen

et al. 2021

International Journal of Gynecological Pathology

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Endocervical adenocarcinomas (ECAs) have been recently reclassified according to their morphologic features linked to etiology by the International Endocervical Adenocarcinoma Criteria and Classification (IECC) and this system is adopted by WHO 2020. This classification separates the ECAs as human papillomavirus (HPV)-associated (HPVA) and HPV-independent (HPVI) subtypes. According to WHO 2020, high risk (HR)-HPV association can be histologically recognized by the presence of luminal mitoses and apoptosis. Therefore, investigating the reproducibility of the morphologic criteria of this new classification will be important in observing the recognizability of tumor types. Full slide sets of 94 ECAs were collected from 4 institutions in Turkey and reclassified on the basis of IECC/WHO 2020 criteria and the presence or absence of HR-HPV. HR-HPV presence was confirmed by HPV DNA in situ hybridization, p16 immunohistochemistry and in conflicted cases with real time-polymerase chain reaction. The final diagnoses were given based on the combination of the histologic evaluation and ancillary test results. Our cohort consisted of 73.4% HPVA and 26.6% HPVI cases. According to the WHO 2020 criteria 92.7% of HPVAs and 88% of HPVIs were easily classified. HPV DNA in situ hybridization was positive in 91.3% of the HPVAs and p16 was positive in all HPVAs, and also positive in 8% of the HPVIs. In conclusion, most of the ECAs can be diagnosed by their characteristic morphologic features by the WHO 2020 criteria. However, we want to emphasize that mitosis/apoptosis criteria may not be helpful especially in mucinous ECAs and ancillary tests for HR-HPV should be used in challenging cases.

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Section: Discussioncontrasting

confidence: 55%

Reproducibility of Morphologic Parameters of the International Endocervical Adenocarcinoma Criteria and Classification System and Correlation With Clinicopathologic Parameters: A Multi-Institutional Study

Bulutay

Haberal

Özen

et al. 2021

International Journal of Gynecological Pathology

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“…A recent meta-analysis by She et al proved that EBV infection is statistically significantly associated with a higher risk for OSCC; in the tumor tissue, DNA regions such as viral oncogene BamH1W have been detected [ 96 ]. Selected viruses that have been found in the oral carcinomas are presented in Table 3 [ 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 ].…”

Section: Viruses Associated With Oral Cancermentioning

confidence: 99%

The Role of the Oral Microbiota in the Etiopathogenesis of Oral Squamous Cell Carcinoma

et al. 2021

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Dysbiosis in the oral environment may play a role in the etiopathogenesis of oral squamous cell carcinoma (OSCC). This review aims to summarize the current knowledge about the association of oral microbiota with OSCC and to describe possible etiopathogenetic mechanisms involved in processes of OSCC development and progression. Association studies included in this review were designed as case–control/case studies, analyzing the bacteriome, mycobiome, and virome from saliva, oral rinses, oral mucosal swabs, or oral mucosal tissue samples (deep and superficial) and comparing the results in healthy individuals to those with OSCC and/or with premalignant lesions. Changes in relative abundances of specific bacteria (e.g., Porphyromonas gingivalis, Fusobacterium nucleatum, Streptococcus sp.) and fungi (especially Candida sp.) were associated with OSCC. Viruses can also play a role; while the results of studies investigating the role of human papillomavirus in OSCC development are controversial, Epstein–Barr virus was positively correlated with OSCC. The oral microbiota has been linked to tumorigenesis through a variety of mechanisms, including the stimulation of cell proliferation, tumor invasiveness, angiogenesis, inhibition of cell apoptosis, induction of chronic inflammation, or production of oncometabolites. We also advocate for the necessity of performing a complex analysis of the microbiome in further studies and of standardizing the sampling procedures by establishing guidelines to support future meta-analyses.

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“…Chronic infections by viruses have been described as a cause of several hematologic and non-hematologic neoplasms [ 99 ]. The presence of the virus is usually determined by immunohistochemistry [ 100 ], immune-fluorometry assay [ 101 ], or PCR on the tumor biopsy [ 100 ]. It can also be detected either by assaying for circulating viral antigens or antibodies directed against the virus [ 102 ] or the presence of the viral genome in the bloodstream [ 103 ].…”

Section: Methodology To Detect Minimal Residual Disease With Ctdnamentioning

confidence: 99%

Liquid Biopsy to Detect Minimal Residual Disease: Methodology and Impact

Honoré

Galot

Marcke

et al. 2021

Cancers

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One reason why some patients experience recurrent disease after a curative-intent treatment might be the persistence of residual tumor cells, called minimal residual disease (MRD). MRD cannot be identified by standard radiological exams or clinical evaluation. Tumor-specific alterations found in the blood indirectly diagnose the presence of MRD. Liquid biopsies thus have the potential to detect MRD, allowing, among other things, the detection of circulating tumor DNA (ctDNA), circulating tumor cells (CTC), or tumor-specific microRNA. Although liquid biopsy is increasingly studied, several technical issues still limit its clinical applicability: low sensitivity, poor standardization or reproducibility, and lack of randomized trials demonstrating its clinical benefit. Being able to detect MRD could give clinicians a more comprehensive view of the risk of relapse of their patients and could select patients requiring treatment escalation with the goal of improving cancer survival. In this review, we are discussing the different methodologies used and investigated to detect MRD in solid cancers, their respective potentials and issues, and the clinical impacts that MRD detection will have on the management of cancer patients.

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Detection of human papillomavirus in oropharyngeal squamous cell carcinoma

Cited by 18 publications

References 83 publications

Reproducibility of Morphologic Parameters of the International Endocervical Adenocarcinoma Criteria and Classification System and Correlation With Clinicopathologic Parameters: A Multi-Institutional Study

Reproducibility of Morphologic Parameters of the International Endocervical Adenocarcinoma Criteria and Classification System and Correlation With Clinicopathologic Parameters: A Multi-Institutional Study

The Role of the Oral Microbiota in the Etiopathogenesis of Oral Squamous Cell Carcinoma

Liquid Biopsy to Detect Minimal Residual Disease: Methodology and Impact

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