Detection of human papillomavirus in non–small cell carcinoma of the lung

Chang, Sing Yun; Keeney, Michael; Law, Mark E.; Donovan, Janis L.; Aubry, Marie Christine; García, Joaquín J.

doi:10.1016/j.humpath.2015.07.012

Cited by 34 publications

(38 citation statements)

References 24 publications

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“…Several authors have concluded that the prevalence of HPV16/18 infection in lung squamous cell carcinoma may therefore suggest a pathogenic mechanism . However, there is significant variability in the rate of HPV‐positive lung cancers reported between studies, and many have argued that a causal relationship cannot be established . Bishop et al assessed HPV status in 220 cases of SPLM SCC following HNSCC, finding 5% of these lung cancers to be HPV‐positive.…”

Section: Discussionmentioning

confidence: 99%

Second primary lung malignancy following head and neck squamous cell carcinoma

et al. 2018

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Objectives/Hypothesis Analyze the characteristics of second primary lung malignancies (SPLMs) following an index head and neck squamous cell carcinoma (HNSCC). Study Design Retrospective cohort study. Methods The Surveillance, Epidemiology and End Results database was queried for all cases of HNSCC between 1973 and 2014 (N = 101,856). This population was compared to a standard population to assess relative risk for lung cancer, calculated as the standardized incidence ratio (SIR). Patients who developed SPLMs were extracted (N = 8,116) and compared to all other cases of lung cancer (N = 1,160,853) to assess histopathological differences. SPLM subpopulations divided by head and neck primary site were compared for lung cancer histology and time interval between cancer diagnoses. Results Overall, 8.0% of HNSCC patients developed SPLMs (SIR = 4.22, P < .001), diagnosed an average of 6.7 years later. Patients with HNSCC of the supraglottis and hypopharynx were at the highest risk relative to a standard population, with SIRs of 8.10 and 6.34, respectively. When comparing SPLMs to all other lung cancers, there was no difference in the distribution of lung lobe affected, but SPLMs were significantly more likely to be of squamous cell carcinoma histology (42.0% vs. 21.0%, P < .001). Among head and neck subsites, lung cancers following larynx tumors had a significantly higher proportion of small cell histology, and those following oropharyngeal or hypopharyngeal tumors had significantly higher proportions of squamous cell histology. Conclusions Patients who undergo curative treatment of HNSCC are at high risk for developing SPLMs. Subsite‐specific differences may help elucidate the degree of risk attributable to smoking, genetic susceptibility, human papillomavirus infection, or metastasis masquerading as an SPLM. Level of Evidence 4 Laryngoscope, 129:903–909, 2019

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Section: Discussionmentioning

confidence: 99%

Second primary lung malignancy following head and neck squamous cell carcinoma

et al. 2018

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“…In addition, this novel ISH-RNA platform is helpful in the diagnosis of emerging pathogens in the absence of a commercial antibodies for IHC. This ISH-RNA technique has been applied in research and diagnostics in human medicine [13,14] while in veterinary medicine, ISH-RNA platform was used in tissues from cattle, pigs, and dogs [15–19] in the context of various infectious diseases.…”

Section: Discussionmentioning

confidence: 99%

A novel RNA-based in situ hybridization to detect Seneca Valley virus in neonatal piglets and sows affected with vesicular disease

2017

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Seneca Valley virus (SVV) is the causative agent of an emerging vesicular disease in swine, which is clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. In addition, SVV has been associated with neonatal mortality in piglets. While a commercial SVV qRT-PCR is available, commercial antibodies are lacking to diagnose SVV infections by immunohistochemistry (IHC). Thus, a novel in situ hybridization technique—RNAscope (ISH) was developed to detect SVVRNA in infected tissues. From a total of 78 samples evaluated, 30 were positive by qRT-PCR and ISH-RNA, including vesicular lesions of affected sows, ulcerative lesions in the tongue of piglets and various other tissues with no evidence of histological lesions. Nineteen samples were negative for SVV by qRT-PCR and ISH-RNA. The Ct values of the qRT-PCR from ISH-RNA positive tissues varied from 12.0 to 32.6 (5.12 x 106 to 5.31 RNA copies/g, respectively). The ISH-RNA technique is an important tool in diagnosing and investigating the pathogenesis of SVV and other emerging pathogens.

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“…Other recent studies also failed to detect hrHPV DNA [Ventana INFORM HPV III Family 16 Probe (B)] and HPV E6/E7 messenger RNA (mRNA) using the RNAscope HPV kit (Advanced Cell Diagnostics Inc., Hayward, CA, USA) in primary lung SqCC and ADC 11, 12. Several other studies consistently have shown no presence of HPV16 E6/E7 mRNA by reverse transcription-PCR (RT-PCR) in primary lung SqCCs and ADCs 13, 14.…”

Section: Discussionmentioning

confidence: 99%

“…However, the sole finding of HPV DNA using PCR is not sufficient to prove the carcinogenic role of HPV in the development of cancer 12, 15. Compared with PCR, the HPV DNA ISH method permits visualization of HPV DNA signals using light microscopy to determine the prevalence of episomal or integrated HPV DNA in tissue specimens.…”

Section: Discussionmentioning

confidence: 99%

“…Although detecting mRNA from FFPE tissue using RT-PCR is challenging, the RNAscope HPV kit (Advanced Cell Diagnostics Inc.) for RNA ISH probes complementary to E6/E7 mRNA permits direct visualization of viral transcripts in tissues [16]. Previous studies comparing HPV DNA ISH and RNA ISH assay found a high concordance rate in head and neck SqCC and nonsmall cell lung carcinoma 4, 12. Therefore, HPV DNA ISH is a feasible detection method in the clinical laboratory to prove that HPV is the etiologic factor for tumor development.…”

Section: Discussionmentioning

confidence: 99%

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No Detection of Episomal or Integrated High-Risk Human Papillomavirus in Nonsmall Cell Lung Carcinomas among Korean Population

Lee

Seong

et al. 2016

Osong Public Health and Research Perspectives

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ObjectivesHigh-risk human papillomavirus (hrHPV) is known to be a representative cancer-causing agent in the genital and head and neck regions. Many studies have detected hrHPV DNA in nonsmall cell lung carcinoma. However, hrHPV–etiologic correlation in nonsmall cell lung carcinoma remains unclear. This study is designed to determine the prevalence of episomal or integrated hrHPV DNA in nonsmall cell lung carcinoma among the Korean population.MethodsSurgically resected nonsmall cell lung carcinoma tissues, including 134 cases of squamous cell carcinoma (SqCC) and 99 cases of adenocarcinoma (ADC), were examined. In situ hybridization (ISH) for detecting episomal or integrated hrHPV DNA was performed using the INFORM HPV III Family 16 Probe (B) in the Ventana-validated assay. Anyplex II HPV28 detection kit based on real-time polymerase chain reaction was used for HPV DNA detection and genotyping.ResultsAll members of the study population were of Korean ethnicity. Episomal or integrated hrHPV DNA ISH analysis result was negative in all 233 cases. One SqCC of 89 samples (42 SqCCs and 47 ADCs) was positive for an hrHPV genotype by Anyplex II HPV28 detection kit.ConclusionOur finding did not demonstrate hrHPV–etiologic correlation in primary lung SqCC and ADC in the Korean population.

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Detection of human papillomavirus in non–small cell carcinoma of the lung

Cited by 34 publications

References 24 publications

Second primary lung malignancy following head and neck squamous cell carcinoma

Second primary lung malignancy following head and neck squamous cell carcinoma

A novel RNA-based in situ hybridization to detect Seneca Valley virus in neonatal piglets and sows affected with vesicular disease

No Detection of Episomal or Integrated High-Risk Human Papillomavirus in Nonsmall Cell Lung Carcinomas among Korean Population

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