Detection of human papillomavirus in oral lesions using commercially developed typing kits

Dhariwal, Sneha; Cubie, Heather; Southam, J.C.

doi:10.1111/j.1399-302x.1995.tb00119.x

Cited by 9 publications

(8 citation statements)

References 25 publications

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“…In 1974, Hausen et al initiated studies that led to the discovery of HPV in carcinoma of the cervix, and other researchers throughout the world have now confirmed this association (5,10,15,18) . Malignancy in many other urogenital and oral locations has now also been related to HPV (3) .…”

Section: Introductionmentioning

confidence: 99%

“…Dhariwal, Cubie and Southam (5) have stated that infections by HPV have been shown to be present in cancers of the mouth and also that the DNA of the Epstein-Barr virus (EBV) has likewise been isolated in these types of lesions, but there is no evidence of their direct action in the process of malign transformation of buccal epithelial cells (2) . Furthermore, Mao et al (12) have emphasized that the inactivation of the p53 protein is the most frequent genetic alteration found in human tumors, premalignant and malignant tumors of the buccal epithelium.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

HPV detection in oral carcinomas

Vidal¹,

Júnior²,

Mello³

et al. 2004

J. Bras. Patol. Med. Lab.

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The authors set out in this study to verify the presence of low-and high-risk DNA of human papillomavirus (HPV) in oral cancer by means of the hybrid capture Digene ® test (São Paulo-SP, Brazil) in smears from exfoliative cytology and also to compare the findings with those of conventional light microscopy (hematoxylin-eosin (HE)/Papanicolaou). Forty individuals gave their written informed consent to participate in the study and also had their clinical data analyzed. The 40 exfoliative cytology examinations performed to date produced the following results: 29 (72.5%) negative for low-and high-risk HPV-DNA; nine (22.5%) positive for low-and high-risk HPV-DNA; one (2.5%) positive for low-risk HPV-DNA; and one (2.5%) positive for high-risk HPV-DNA. There was agreement among the findings for the presence of DNA-HPV for both exfoliative cytology (smear to hybrid capture Digene ® test and the cytological smear readings made by conventional light microscopy). It was therefore concluded that the HPV virus may be a cocarcinogen of the mouth cancer as it is in the cervix cancer. Oral cancer HPV Exfoliative cytology Hybrid capture resumo Os autores buscaram verificar, neste estudo, a presença do papilomavírus humano (HPV) de baixo e de alto risco em carcinomas orais através do teste de captura híbrida Digene ® (São Paulo-SP, Brasil) em amostras colhidas pela citologia esfoliativa bucal e, ainda, avaliar comparativamente as referidas leituras com alterações celulares indicativas deste vírus obtidas com a interpretação citológica óptica convencional (hematoxilinaeosina (HE)/Papanicolaou). Quarenta indivíduos concordaram, espontaneamente, através de assinatura do termo de consentimento livre e esclarecido, em participar da pesquisa, e seus dados clínicos foram analisados. Entre as 40 amostras provenientes da citologia esfoliativa 29 (72,5%) mostraram-se negativas para presença de HPV-DNA de baixo e de alto risco; nove (22,5%) foram positivas para o HPV-DNA de baixo e de alto risco; uma (2,5%) foi positiva apenas para o HPV de baixo risco; e também uma (2,5%) foi positiva apenas para o HPV de alto risco. Houve concordância entre todos os resultados positivos para presença de HPV-DNA nas amostras citológicas submetidas ao teste de captura híbrida Digene ® e na leitura de esfregaço citológico ao microscópio óptico convencional. Desta forma, conclui-se que o vírus HPV pode comportar-se como mais um co-carcinógeno para o câncer de boca, à semelhança do carcinoma uterino.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

HPV detection in oral carcinomas

Vidal¹,

Júnior²,

Mello³

et al. 2004

J. Bras. Patol. Med. Lab.

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“…Commercially available DNA hybridization kits are approved for cervical specimens; this technology has been used successfully to identify strains of HPV in intraoral lesions. 24 In biopsy specimens, the pathological interpretation can be corroborative but may not always be definitively diagnostic. Clinical features alone are usually sufficient to make the diagnosis in adults with typical genital condylomata acuminata.…”

Section: Commentmentioning

confidence: 99%

Oral Condylomata in Children

Squires¹,

Persaud²,

Simon³

et al. 1999

Arch Pediatr Adolesc Med

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“…Characteristic features include koilocytosis, acanthosis and papillomatosis which, coupled to the clinical appearance, suggest the infection (14). The classical oral lesions associated with human papillomavirus are squamous cell papilloma, condyloma acuminatum, verruca vulgaris and focal epithelial hyperplasia (15,16).…”

Section: Diagnosismentioning

confidence: 99%

HPV and oral lesions: preventive possibilities, vaccines and early diagnosis of malignant lesions

Testi

Nardone

et al. 2015

ORL

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SUMMARYThe importance of HPV in world healthy is high, in fact high-risk HPV types contribute significantly to viral associated neoplasms.In this article we will analyze vary expression of HPV in oral cavity both benign and malignant, their prevalence and the importance in early diagnosis and prevention. The classical oral lesions associated with human papillomavirus are squamous cell papilloma, condyloma acuminatum, verruca vulgaris and focal epithelial hyperplasia. Overall, HPV types 2, 4, 6, 11, 13 and 32 have been associated with benign oral lesions while HPV types 16 and 18 have been associated with malignant lesions, especially in cancers of the tonsils and elsewhere in the oropharynx. Transmission of the virus can occur with direct contact, genital contact, anal and oral sex; latest studies suggest a salivary transmission and from mother to child during delivery. The number of lifetime sexual partners is an important risk factor for the development of HPV-positive head-neck cancer. Oral/oropharyngeal cancer etiologically associated with HPV having an increased survival and a better prognostic (85%-90% to five years). There is no cure for the virus. There are two commercially available prophylactic vaccines against HPV today: the bivalent (16 and 18) Cervarix® and the tetravalent (6,11, 16 and 18) Gardasil® and new vaccine Gardasil 9 (6,11,16,18,31,33, 45, 52, 58) was approved in the United States. To be effective, such vaccination should start before "sexual puberty". The vaccine could be an important preventive strategy, in fact the scientific community is in agreement on hypothesis that blocking the contagion it may also limit the distance complications as the oropharyngeal cancer.

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Detection of human papillomavirus in oral lesions using commercially developed typing kits

Cited by 9 publications

References 25 publications

HPV detection in oral carcinomas

HPV detection in oral carcinomas

Oral Condylomata in Children

HPV and oral lesions: preventive possibilities, vaccines and early diagnosis of malignant lesions

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