Detection of Human Papillomavirus L1 Protein in Condylomata Acuminata From Various Anatomical Sites

Wools, Kara; Bryan, Janine T.; Katz, Barry P.; Rodriguez, Michael; Davis, Thomas; Brown, Darron R.

doi:10.1097/00007435-199403000-00009

Cited by 4 publications

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“…Since immunohistochemical staining was reported to have very low sensitivity until the early 1990s, there have been few reports on immunohistochemical staining for wart lesions in the dermatological area 3 . On the contrary, immunohistochemical staining has been actively used in the area of obstetrics and gynecology as a tool for detecting HPV in various genital dysplasias, and many recent reports have been published without much concern over the method's sensitivity.…”

Section: Discussionmentioning

confidence: 99%

“…In general, immunohistochemical staining and polymerase chain reaction (PCR) are used as the representative methods of virus detection 2 . The HPV detection rate of immunohistochemical staining in a wart lesion on the skin and mucous membrane was known to have very low sensitivity until the early 1990s, and in 1994 Wools et al 3 reported that only 20% of genital warts were positive for L1 capsid protein on immunohistochemical staining. Since then there has been no dermatological report of HPV detection in wart lesions using immunohistochemical staining.…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemistry and Polymerase Chain Reaction for Detection Human Papilloma Virus in Warts: A Comparative Study

Lee

Choo

et al. 2016

Ann Dermatol

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BackgroundImmunohistochemistry and polymerase chain reaction (PCR) are the most widely used methods for the detection of viruses. PCR is known to be a more sensitive and specific method than the immunohistochemical method at this time, but PCR has the disadvantages of high cost and skilled work to use widely. With the progress of technology, the immunohistochemical methods used in these days has come to be highly sensitive and actively used in the diagnostic fields.ObjectiveTo evaluate and compare the usefulness of immunohistochemistry and PCR for detection human papilloma virus (HPV) in wart lesions.MethodsNine biopsy samples of verruca vulgaris and 10 of condyloma accuminatum were examined. Immunohistochemical staining using monoclonal antibody to HPV L1 capsid protein and PCR were done for the samples. DNA sequencing of the PCR products and HPV genotyping were also done.ResultsHPV detection rate was 78.9% (88.9% in verruca vulgaris, 70.0% in condyloma accuminatum) on immunohistochemistry and 100.0% for PCR. HPV-6 genotype showed a lower positivity rate on immunohistochemistry (50.0%) as compared to that of the other HPV genotypes.ConclusionImmunohistochemistry for HPV L1 capsid protein showed comparable sensitivity for detection HPV. Considering the high cost and great effort needed for the PCR methods, we can use immunohistochemistry for HPV L1 capsid protein with the advantage of lower cost and simple methods for HPV detection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immunohistochemistry and Polymerase Chain Reaction for Detection Human Papilloma Virus in Warts: A Comparative Study

Lee

Choo

et al. 2016

Ann Dermatol

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“…1,[35][36][37][38][39][40] As mentioned previously, the majority of these articles used pan-HPV antibodies. [36][37][38][39][40] These pan-HPV antibo- the majority of these were genital lesions diagnostic of condyloma or "consistent with" condyloma. They found that HPV IHC (Abcam antibody) had 91% sensitivity and 86% specificity when compared to ISH.…”

Section: Comments and Conclusionmentioning

confidence: 99%

“…We identified 7 relevant articles assessing HPV detection by IHC in cutaneous lesions . As mentioned previously, the majority of these articles used pan‐HPV antibodies . These pan‐HPV antibodies have lower sensitivity and it is unclear which HPV subtypes they target.…”

Section: Comments and Conclusionmentioning

confidence: 99%

Review of the current medical literature and assessment of current utilization patterns regarding human papillomavirus in situ hybridization and immunohistochemistry in dermatopathology

2017

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Human papillomaviruses have been implicated in many cutaneous diseases. Practicing dermatopathologists often consider using immunohistochemistry and in situ hybridization to help clarify the histologic diagnosis, particularly in cases with borderline or nondiagnostic features. We reviewed the current evidence behind the use of these two techniques in dermatopathology.We identified only two studies utilizing the currently available immunohistochemical antibodies. We found more evidence regarding the use of in situ hybridization; however, the majority of this evidence focuses on diagnosing condylomas and other lesions of the genital skin. We also assessed current utilization patterns of attendees of the American Society of Dermatopathology annual meeting (Chicago, 2016) which revealed a wide spectrum of current utilization ranging from no use to regular use more than once per month. Two-thirds of respondents utilized these tests primarily when requested by the submitting clinician and one-third of the respondents utilize these tests reflexively in specific clinical scenarios. The AUC Task Force chose to evaluate the use of HPV ISH and IHC in dermatopathology. We chose these tests because they are often used by dermatopathologists and also requested by our clinical colleagues. In addition, we felt that there was a broad spectrum of current utilization of these tests ranging from dermatopathologists who never or rarely perform these studies to labs that routinely order them in specific situations.HPV are double-stranded, nonenveloped deoxyribonucleic acid (DNA) viruses of the papovirus family. Over 100 subtypes have been described to date. These viruses have been implicated in many cutaneous diseases including: verruca, condyloma, verrucous carcinoma, squamous cell carcinoma (SCC) and squamous cell carcinoma in situ (SCCIS) of the nail unit, SCC/SCCIS of the genital skin and SCC/SCCIS in immunocompromised patients.With respect to HPV ISH, many commercially available typespecific probes and "cocktails" are available; however, type-specific probes for HPV 6,11,16,18,31 and

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Some clear answers regarding transmission of genital human papillomavirus

Dillner

2019

The Lancet Infectious Diseases

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Detection of Human Papillomavirus L1 Protein in Condylomata Acuminata From Various Anatomical Sites

Cited by 4 publications

References 0 publications

Immunohistochemistry and Polymerase Chain Reaction for Detection Human Papilloma Virus in Warts: A Comparative Study

Immunohistochemistry and Polymerase Chain Reaction for Detection Human Papilloma Virus in Warts: A Comparative Study

Review of the current medical literature and assessment of current utilization patterns regarding human papillomavirus in situ hybridization and immunohistochemistry in dermatopathology

Some clear answers regarding transmission of genital human papillomavirus

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