Detection of human papillomavirus type 6/11 DNA in conjunctival papillomas by in situ hybridization with radioactive probes

McDonnell, Peter J.; McDonnell, Jan M.; Kessis, Ted; Green, W. Richard; Shah, Keerti V.

doi:10.1016/s0046-8177(87)80378-7

Cited by 88 publications

(46 citation statements)

References 29 publications

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“…In addition, HPV was detected in all five papilloma cases studied by Saegusa et al, 19 and in 9 of 10 papilloma cases (90%) studied by Gallagher et al 3 The presence of HPV in papilloma has also been confirmed by Southern blotting and in situ hybridization. 20 Based on these reports and our data, it is likely that HPV is closely involved in the pathogenesis of conjunctival papillomas. One of the advantages of HC-II is its ability to estimate viral genome copy number from the magnitude of the RLU value obtained.…”

Section: Discussionsupporting

confidence: 68%

Detection of human papillomavirus in pterygium and conjunctival papilloma by hybrid capture II and PCR assays

Takamura

Kubo

Tsuzuki

et al. 2008

Eye

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Aim To elucidate the putative role of human papillomavirus (HPV) infection in pterygium and conjunctival papilloma. Methods Hybrid capture II (HC-II) and polymerase chain reaction (PCR) assays were performed to detect HPV in pterygium (42 samples obtained from 40 patients) and conjunctival papilloma (8 samples from 6 patients). The amount of HPV DNA was evaluated by measurement of relative light units (RLUs) on a luminometer. Results All papilloma samples were positive for HPV DNA by PCR and HC-II. The RLU values for specimens of recurrent and re-recurrent papilloma were markedly higher than those for specimens of primary lesions. HPV was detected by PCR in 2 of 42 (4.8%) b-globin-positive pterygium specimens, whereas HC-II showed that HPV was negative in all pterygium samples. Conclusions Our results support the hypothesis that HPV DNA is associated with the pathogenesis of conjunctival papilloma, but not pterygium. RLU measurement by HC-II may serve as a marker for evaluating the activity of HPV in conjunctival tumours.

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Section: Discussionsupporting

confidence: 68%

Detection of human papillomavirus in pterygium and conjunctival papilloma by hybrid capture II and PCR assays

Takamura

Kubo

Tsuzuki

et al. 2008

Eye

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“…The aetiology of the conjunctival papilloma is not known, but it has been associated with human papillomavirus (HPV) types 6,11 and 16 (McDonnell et al 1987;Saegusa et al 1995). HPV types 16 and 18 have in addition been found in normal conjunctival tissue (Karcioglu 1997).…”

Section: Discussionmentioning

confidence: 99%

“…The exophytic configuration may be sessile or pedunculated. Inverted papillomas of the conjunctiva are very rare (Streeten et al 1979).Dysplasia may be observed, but carcinoma rarely develops in a conjunctival papilloma (Zimmerman 1980).In a study of 1016 epibulbar lesions (Ash 1950) the conjunctival papilloma was found to be the second most frequent epithelial lesion and the conjunctival papilloma is an equally frequent lesion of the caruncle (Santos & Gó mez-Leal 1994).The aetiology of the conjunctival papilloma is not known, but it has been associated with human papillomavirus (HPV) types 6,11 and 16 (McDonnell et al 1987;Saegusa et al 1995). HPV types 16 and 18 have in addition been found in normal conjunctival tissue (Karcioglu 1997).…”

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confidence: 99%

Conjunctival papilloma

Sjö

Heegaard

Prause

2000

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To determine morphological and clinical characteristics of conjunctival papilloma. Methods: Specimens of conjunctival papilloma registered during the period 1983-1997 were collected. For each case the patient's sex and age, number of papillomas/patient, seasonal debut, duration, number of recurrences, localisation and presence of dysplasia and/or inflammation in the papilloma were registered. Results: A total of 245 papillomas in 219 patients were found. 132 (60.3%) of the patients were males. The age distribution showed skewness to the left with a peak in the age groups 20-29 years and 30-39 years. No variation of seasonal debut was found. 14 patients (6%) had more than one papilloma concurrently. 24 (11%) of the patients had recurrence. Most of the papillomas were located medially and inferiorly. 98 (40%) of the papillomas were inflamed. 14 (6%) of the papillomas were dysplastic. Conclusion:The conjunctival papilloma is a common tumour. More males than females develop conjunctival papillomas and the incidence is highest among patients aged 20-39 years. The conjunctival papilloma is primarily located medially and inferiorly.Key words: papilloma -conjunctiva -localisation -dysplasia -sex -age -distribution. T he squamous cell papilloma of the conjunctiva is a benign and common epithelial tumour.Conjunctival papillomas may have an exophytic, mixed or inverted growth pattern (Conlon et al. 1994;Streeten et al. 1979). The exophytic configuration may be sessile or pedunculated. Inverted papillomas of the conjunctiva are very rare (Streeten et al. 1979).Dysplasia may be observed, but carcinoma rarely develops in a conjunctival papilloma (Zimmerman 1980).In a study of 1016 epibulbar lesions (Ash 1950) the conjunctival papilloma was found to be the second most frequent epithelial lesion and the conjunctival papilloma is an equally frequent lesion of the caruncle (Santos & Gó mez-Leal 1994).The aetiology of the conjunctival papilloma is not known, but it has been associated with human papillomavirus (HPV) types 6,11 and 16 (McDonnell et al. 1987;Saegusa et al. 1995). HPV types 16 and 18 have in addition been found in normal conjunctival tissue (Karcioglu 1997). Material and MethodsThe files at the Eye Pathology Institute, University of Copenhagen, Denmark were reviewed for the period 1983 to 1997, included. Specimens diagnosed as a conjunctival papilloma were identified.The following data were registered from the pathology referral forms: the patient's sex and age, number of papillomas/patient, seasonal debut of the papilloma, duration, number of recurrences, the affected eye (right or left), localisation and presence of dysplasia and/or inflammation in the papilloma.A chi-squared test was used in order to test for associations between the following parameters: sex and localisation of the papilloma, localisation and affected eye and sex and age-distribution. A probability level of pϽ0.05 was considered significant. ResultsA total number of 245 papillomas in 219 patients were registered. The chi-squared...

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“…Even CASE REPORT 1,2 Postgraduate (3rd Year), 3 Professor and Head, 4 Associate Professor [1][2][3][4] Department of Ophthalmology, Rohilkhand Medical College and Hospital, Bareilly, Uttar Pradesh, India though benign they carry profound malignancy potential and can present at numerous anatomical locations, such as skin, cervix, esophagus, respiratory tract, breast duct, etc. Commonly referred as warts or genital warts when present over skin or genitals, the papillomas can be pedunculated or sessile.…”

Section: Introductionmentioning

confidence: 99%

“…Polymerase chain reaction studies have highlighted the association between HPV type and site and cell specificity for papilloma; e.g., HPV 6 & 11 for skin and genital warts, HPV 6a & 45 for conjunctival papilloma. 4 Infectious role of HPV explains the multiple sites or crop-like involvement of papillomas.…”

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confidence: 99%

Squamous Papilloma over Lid Margin: An Uncommon Presentation

Goyal¹,

Gupta²,

Dokania³

et al. 2016

International Journal of Advanced and Integrated Medical Sciences

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Papilloma is a histopathological term describing tumors of a classical finger-like or cauliflower morphology. Though benign, they carry profound malignancy potential and can present at numerous anatomical locations, such as skin, cervix, esophagus, respiratory tract, breast duct, etc. Papillomas over conjunctiva are not frequently encountered and carry special significance.A 60-year-old diabetic female presented with a left upper lid marginal sessile mass of 9 months duration with mild epiphora and foreign body sensation. On examination, a solitary, fleshy, sessile, polypoidal mass measuring 1 × 1 cm was seen, with irregular cauliflower-like surface present over lateral aspect of left upper lid, firm in consistency with raised edges. Visual acuity in right eye was 6/186/9 and left eye 6/366/9, intraocular pressure (IOP) was 14.6 mm Hg in both eyes by Schiötz tonometry. Complete excision of the lesion employing microsurgical technique with close apposition of lid margin using 6-0 Vicryl sutures was done under local anesthesia (LA). Histopathological examination revealed stratified squamous epithelium forming papillae with variable goblet cells supported by prominent branching fibrovascular cores in the center. A satisfactory cosmetic result was achieved. Follow-up of 6 months did not reveal any recurrence. A satisfactory functional and cosmetic result achieved.Squamous papillomas over lid margin are uncommon presentations. Even though benign they carry morbidity potential in the form of chronic epiphora, corneal erosions, and lid deformities if inadequately managed.

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Detection of human papillomavirus type 6/11 DNA in conjunctival papillomas by in situ hybridization with radioactive probes

Cited by 88 publications

References 29 publications

Detection of human papillomavirus in pterygium and conjunctival papilloma by hybrid capture II and PCR assays

Detection of human papillomavirus in pterygium and conjunctival papilloma by hybrid capture II and PCR assays

Conjunctival papilloma

Squamous Papilloma over Lid Margin: An Uncommon Presentation

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