Detection of human papillomaviruses in squamous cell carcinomas of the lung

Papadopoulou, Katerina; Labropoulou, Vassiliki; Davaris, Panagiotis; Mavromara, Penelope; Tsimara-Papastamatiou, H.

doi:10.1007/s004280050215

Cited by 33 publications

(22 citation statements)

References 39 publications

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“…A Greek study also showed such an association (Papadopoulou et al, 1998). We also observed pearl formation in 4 out of 8 well-differentiated SQCs (50%).…”

Section: Discussionsupporting

confidence: 78%

Human papillomavirus-16 is integrated in lung carcinomas: a study in Chile

et al. 2007

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The human papillomavirus (HPV) was detected in 20 (29%) out of 69 lung carcinomas (LCs) in Chile, by PCR and Southern blot, and was more frequently detected in squamous cell carcinoma (SQC) than in adenocarcinomas (46 vs 9%, P ¼ 0.001). HPV-16, positive in 11 cases, was the most frequently detected HPV genotype determined by DNA sequencing. HPV-16 E2/E6 ratio, estimated from real-time PCR analysis, was much lower than the unity, suggesting that at least a partial HPV-16 genome was integrated in all but one HPV-16-positive SQCs. The remaining one case was suspected to have only episomal HPV-16. Although the viral load was low in most of the LCs, a case showed the HPV-16 copy number as high as 8479 per nanogram DNA, which was even a few times higher than the minimum viral load of seven cervical carcinomas (observed viral load: 3356 -609 392 per nanogram DNA). The expression of the HPV-16/18 E6 protein was found in only two HPV-16-positive SQCs (13%) but not in the case with the highest viral load. Although the viral load was in general very low and HPV E6 expression is none or weak, further studies seem warranted to examine aetiological involvement of high-risk HPV in lung carcinogenesis.

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“…A Greek study also showed such an association (Papadopoulou et al, 1998). We also observed pearl formation in 4 out of 8 well-differentiated SQCs (50%).…”

Section: Discussionsupporting

confidence: 78%

Human papillomavirus-16 is integrated in lung carcinomas: a study in Chile

et al. 2007

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“…High and low incidence regions are found in southern and northern European countries alike. In particular, the two studies from Athens, one reporting 0% and the other 69% are confusing [33,34]. They can neither be explained by the cancer type (both analyzed SCC, in one study also ADC and LCLC were investigated), material (both used frozen and FFPE samples) or the methodology (both studies used PCR, once in conjunction with a Southern blot technique while the other additionally applied in situ hybridization).…”

Section: Discussionmentioning

confidence: 92%

Incidence of human papilloma virus in lung cancer

2009

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“…Diagnosis of various infectious diseases can be efficiently supported by the application of molecular analysis [3,13,18]. For specific detection of mycobacterial DNA, amplification of the ribosomal 16S rRNA-fragment gene [6,11], the 65-kDa antigen gene [21,22], the insertion fragment IS986/6110 [4,19] and the fragment MTP40 [8,10] have proved useful.…”

Section: Introductionmentioning

confidence: 99%

Quality assessment in diagnostic molecular pathology: experience from a German-Austrian-Swiss multicenter trial

Cabras¹,

Kremer²,

Schulz³

et al. 2000

Virchows Archiv

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In order to assess the current technical standard of diagnostic molecular pathology, we have conducted a multicenter trial with 34 participating pathology laboratories in Germany, Austria and Switzerland. Formalin-fixed, paraffin-embedded tissue blocks were selected from 15 cases, comprising 4 B-cell non-Hodgkin's lymphomas, 4 T-cell non-Hodgkin lymphomas, 4 cases with lymphadenitis, 2 cases with confirmed tuberculosis and 1 case of sarcoidosis. All participating laboratories received one 10-microm section from each of the 15 cases to detect clonality using immunoglobulin heavy chain (IgH) gene or T-cell receptor (TCR)-gamma gene rearrangement analysis in 12 and mycobacterial DNA in 3 cases. In addition, participants had to answer technical questions about the application of internal quality controls and performance of fragment length or sequence analysis. Correct results were reported in 80% and 90% for IgH and TCR-gammagene rearrangement analysis, respectively, and in 83% for mycobacterial DNA analysis. No significant differences in the quality of results were obvious when the individual techniques used for molecular analysis were compared. However, when two independent techniques were used by the same laboratory, a higher rate of correct results was obtained for IgH and TCR rearrangement analysis. In conclusion, this study demonstrates a high technical standard of molecular diagnostic adjuncts among the participating laboratories. Regular multicenter trials with a greater number of participating laboratories working in this field will be indispensable to ensure a continuing or increasing standard in diagnostic molecular pathology.

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Detection of human papillomaviruses in squamous cell carcinomas of the lung

Cited by 33 publications

References 39 publications

Human papillomavirus-16 is integrated in lung carcinomas: a study in Chile

Human papillomavirus-16 is integrated in lung carcinomas: a study in Chile

Incidence of human papilloma virus in lung cancer

Quality assessment in diagnostic molecular pathology: experience from a German-Austrian-Swiss multicenter trial

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