Detection of hydrogen peroxide with luminol electrogenerated chemiluminescence at mesoporous platinum electrode in neutral aqueous solution

Tailoring Catalytic Activity of Pt Nanoparticles Encapsulated Inside Dendrimers by Tuning Nanoparticle Sizes with Subnanometer Accuracy for Sensitive Chemiluminescence-Based Analyses

“…To demonstrate the feasibility of the Pt DENs (particularly G6-NH 2 (Pt 200 ) because its activity is the highest among the Pt DENs explored in the present study) in sensitive analytical applications, we first investigated the effects of the reaction conditions (including the pH, concentration of luminol, and concentration of G6-NH 2 (Pt 200 ) DEN) on the enhanced chemiluminescence of the luminol/H 2 O 2 system in the presence of the G6-NH 2 (Pt 200 ) DENs (Supporting Information, Figure S8). Figure S8a shows that the chemiluminescence intensity of luminol/H 2 O 2 is dependent on the pH values of the reaction solution containing the G6-NH 2 (Pt 200 ) DENs, which is consistent with previously reported nanoparticles enhancing the chemiluminescence of luminol/H 2 O 2 . , For example, the chemiluminescence intensity reached its maximal values under strong alkaline conditions, specifically at pH 13 or 11.5 with gold or cupric oxide nanoparticles, respectively. , However, compared to the previously reported nanoparticle catalysts, the maximum chemiluminescence was achieved under a relatively weak alkaline condition (i.e., pH 9.5) with the G6-NH 2 (Pt 200 ) DENs, which would make the DENs more beneficial for their direct applications in the analysis of biological samples that require mild assay conditions . The chemiluminescence of luminol/H 2 O 2 was also dependent on the concentration of luminol as shown in Figure S8b.…”

Electrogenerated Chemiluminescence of Luminol Mediated by Carbonate Electrochemical Oxidation at a Boron-Doped Diamond

“…While this is an organic molecule and could be part of this category, luminol cannot be regarded as a strict ECL luminophore because it is not regenerated after the light emission. However, the ECL of luminol is gaining high interest as it might be a substitute for more expensive inorganic complexes in many research activities within biosensing. − Furthermore, a multiplicity of mechanistic investigations exist for different electrode materials such as Pt, Au, , indium tin oxide, and carbon-based materials. − Whether the trigger to achieve light emission is different, such as electrochemical or chemical for ECL or CL, respectively, the mechanisms leading to the excited states show many similarities. CL emission is induced by the addition of hydrogen peroxide (H 2 O 2 ) and a suitable catalyst, such as metal ions (e.g., Co 2+ , Fe 2+ , or Cu 2+ ) − or heme proteins (e.g., hemoglobin and peroxidases). , The hydroxyl radicals (OH • ) generated from H 2 O 2 by the catalyst trigger the luminol oxidation, which leads to the formation of 3-aminophthalate dianion (3AP) in its triplet state.…”

“…However, the ECL of luminol is gaining high interest as it might be a substitute for more expensive inorganic complexes in many research activities within biosensing. 10−17 Furthermore, a multiplicity of mechanistic investigations exist for different electrode materials 18 such as Pt, 19 Au, 20,21 indium tin oxide, 22 and carbon-based materials. 23−25 Whether the trigger to achieve light emission is different, such as electrochemical or chemical for ECL or CL, respectively, the mechanisms leading to the excited states show many similarities.…”

Kinetics Simulation of Luminol Chemiluminescence Based on Quantitative Analysis of Photons Generated in Electrochemical Oxidation

“…Electrogenerated chemiluminescence, or electrochemiluminescence (ECL), has been used in a variety of fields such as clinical diagnostics, immunological analysis, and environmental monitoring due to its simplicity and high efficiency. , Luminol (5-amino-2,3-dihydro-1,4-phthalazinedione) is one of the most popular ECL reagents, and a large number of studies for analytical applications have been carried out by utilizing both anodic and cathodic polarized electrodes. − Since luminescence is produced by the reaction of oxidized luminol with reactive oxygen species such as H 2 O 2 and O 2 – , the mechanism of ECL of luminol involves many reaction processes. In spite of the complex reaction mechanism, cathodic ECL was employed with several kinds of electrodes, such as C-doped TiO 2 , niobate semiconductor, and indium tin oxide, for sensing reactive oxygens in bioactive compound.…”