Detection of hydrogen peroxide with chemiluminescent micelles

Lee, Dongwon; Erigala, Venkata Reddy; Dasari, Madhuri; Yu, Junhua; Dickson, Robert M.; Murthy, Niren

doi:10.2147/ijn.s3728

Cited by 69 publications

(38 citation statements)

References 20 publications

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“…In this study, the molar ratio of cyclohexanedimethanol to HBA was decided after the careful consideration of stability in aqueous environments and reactivity to H 2 O 2 [6,16]. It has been reported that the aromatic peroxalate compounds instantaneously react with H 2 O 2 specifically to decompose into carbon dioxide, and rapidly degrade under aqueous conditions via hydrolysis [6,23,24]. In contrast, polyoxalate with aliphatic peroxalate ester has low reactivity to H 2 O 2 and undergo slow hydrolytic degradation under aqueous conditions.…”

Section: Discussionmentioning

confidence: 99%

Reduction of oxidative stress by p-hydroxybenzyl alcohol-containing biodegradable polyoxalate nanoparticulate antioxidant

et al. 2011

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Section: Discussionmentioning

confidence: 99%

Reduction of oxidative stress by p-hydroxybenzyl alcohol-containing biodegradable polyoxalate nanoparticulate antioxidant

et al. 2011

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“…For instance, peroxalate nanoparticles were fabricated and used as a chemiluminescence probe to detect H 2 O 2 activity in inflammation and infection model in vivo. 18,45,46 Oxazine conjugated nanoparticles have been synthesized and used for ex vivo detection of . Fibrin-affinity probes were used to assess acute implant-mediated edema formation and fibrin deposition.…”

Section: Imaging the Release Of Inflammatory Productsmentioning

confidence: 99%

Non-invasive Characterization of Immune Responses to Biomedical Implants

Zhou

Tang

2015

Ann Biomed Eng

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Biomedical implants play an important role in today's clinical practice. Unfortunately, biomedical implant-mediated host responses may lead to implant failure. Thus, all implants are tested for tissue compatibility prior to clinical trials. For that, after implantation in animals for different periods of time, the implants and surrounding tissues are isolated for histological analyses. Unfortunately, histological evaluation methods are labor intensive, time consuming, expensive and do not produce quantitative outcomes. With the advent of in vivo imaging technology, many imaging methods have been developed for evaluating biomedical implant-associated immune responses. In this review, we summarize the recent progress in the use of in vivo real-time imaging techniques for assessing acute phase foreign body reactions, including fibrin deposition, inflammatory cell recruitment and responses surrounding biomaterial implants. These new technologies may serve as powerful tools to characterize tissue compatibility of medical implants.

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“…Bioanalytical approaches for detection of H 2 O 2 described in the literature include chemiluminescence, 6 fluorescence, 7 and electrochemistry. 8,9 The latter approach is particularly attractive due to simplicity and reliability of measurements, and the possibility for miniaturization.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical biosensors for on-chip detection of oxidative stress from immune cells

et al. 2011

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Seamless integration of biological components with electrochemical sensors is critical in the development of microdevices for cell analysis. The present paper describes the integration miniature Au electrodes next to immune cells (macrophages) in order to detect cell-secreted hydrogen peroxide (H2O2). Photopatterning of poly(ethylene glycol) (PEG) hydrogels was used to both immobilize horseradish peroxidase molecules onto electrodes and to define regions for cell attachment in the vicinity of sensing electrodes. Electrodes micropatterned in such a manner were enclosed inside poly(dimethylsiloxane) fluid conduits and incubated with macrophages. The cells attached onto the exposed glass regions in the vicinity of the electrodes and nowhere else on the non-fouling PEG hydrogel surface. A microfluidic device was converted into an electrochemical cell by placing flow-through Ag/AgCl reference and Pt wire counter electrodes at the outlet and inlet, respectively. This microdevice with integrated H2O2-sensing electrodes had sensitivity of 27 μA/cm2 mM with a limit of detection of 2 μM. Importantly, this microdevice allowed controllable seeding of macrophages next to electrodes, activation of these cells and on-chip monitoring of H2O2 release in real time. In the future, this biosensor platform may be utilized for monitoring of macrophage responses to pathogens or for the study of inflammatory signaling in micropatterned cell cultures.

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Detection of hydrogen peroxide with chemiluminescent micelles

Cited by 69 publications

References 20 publications

Reduction of oxidative stress by p-hydroxybenzyl alcohol-containing biodegradable polyoxalate nanoparticulate antioxidant

Reduction of oxidative stress by p-hydroxybenzyl alcohol-containing biodegradable polyoxalate nanoparticulate antioxidant

Non-invasive Characterization of Immune Responses to Biomedical Implants

Electrochemical biosensors for on-chip detection of oxidative stress from immune cells

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