Detection of Hydroxyl Radicals by -Phenylalanine Hydroxylation: A Specific Assay for Hydroxyl Radical Generation in Biological Systems

Biondi, Roberto; Xia, Yong; Rossi, Ruggero; Paolocci, Nazareno; Ambrosio, Giuseppe; Zweíer, Jay L.

doi:10.1006/abio.2000.4958

Cited by 29 publications

(24 citation statements)

References 35 publications

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“…Many of the pathophysiological effects of hydrogen peroxide can be prevented by a 15-min pretreatment with 0.2 mmol/l salicylaldehyde isonicotinoyl hydrazone (SIH). This novel lipophilic iron chelator is known to attenuate the oxidative stress of hydrogen peroxide by reducing its ability to release hydroxyl radical via the Fenton reaction (2,14).…”

Section: Hydrogen Peroxide and Acetaminophenmentioning

confidence: 99%

“…However, acetaminophen is able to attenuate the production of hydroxyl radicals during reperfusion (21). Thus it seems reasonable to suggest that the protective effects of acetaminophen against hydrogen peroxide are mediated via a similar mechanism, i.e., by inhibition of the actions of hydroxyl radicals released from hydrogen peroxide in the Fenton reaction (2,14).…”

Section: Hydrogen Peroxide and Acetaminophenmentioning

confidence: 99%

“…Another purpose was to investigate several antioxidant mechanisms that might help explain the cardioprotective properties of acetaminophen, e.g., does acetaminophen attenuate the negative inotropic actions of exogenously administered hydrogen peroxide? The latter objective seemed important because it is well known that hydrogen peroxide releases hydroxyl radical via the Fenton reaction (2,14), and it has already been shown that acetaminophen attenuates the production of hydroxyl radical in the postischemic-reperfused myocardium (21).…”

mentioning

confidence: 99%

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Acetaminophen and low-flow myocardial ischemia: efficacy and antioxidant mechanisms

Merrill

2002

American Journal of Physiology-Heart and Circulatory Physiology

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Merrill, Gary F. Acetaminophen and low-flow myocardial ischemia: efficacy and antioxidant mechanisms. Am J Physiol Heart Circ Physiol 282: H1341-H1349, 2002; 10.1152/ajpheart.00716.2001.-In the current study, the cardioprotective efficacy of 0.35 mmol/l acetaminophen administered 10 min after the onset of a 20-min period of global, low-flow myocardial ischemia was investigated. Matched control hearts were administered an equal volume of KrebsHenseleit physiological buffer solution (vehicle). In separate groups of hearts, the concentration-dependent, negative inotropic properties of hydrogen peroxide and the ability of acetaminophen to attenuate these actions, as well as the effects of acetaminophen on ischemia-reperfusion-mediated protein oxidation, were studied. Acetaminophen-treated hearts regained a significantly greater fraction of baseline, preischemia control function during reperfusion than vehicle-treated hearts. For example, contractility [rate of maximal developed pressure in the left ventricle (ϮdP/dtmax)] after 10 min of reperfusion was 109 Ϯ 24 and 42 Ϯ 9 mmHg/s (P Ͻ 0.05), respectively, in the two groups. The corresponding pressure-rate products were 1,840 Ϯ 434 vs. 588 Ϯ 169 mmHg ⅐ beats ⅐ min Ϫ1 (P Ͻ 0.05). Acetaminophen attenuated peroxynitrite-mediated chemiluminescence in the early minutes of reperfusion (e.g., at 6 min, corresponding values for peak light production were ϳ8 ϫ 10 6 counts/min for vehicle vs. Ͻ4 ϫ 10 6 counts/min for acetaminophen, P Ͻ 0.05) and the negative inotropic effects of exogenously administered hydrogen peroxide (e.g., at 0.4 mmol/l hydrogen peroxide, pressure-rate products were ϳ1.0 ϫ 10 4 and 3.8 ϫ 10 3 mmHg ⅐ beats ⅐ min Ϫ1 in acetaminophen-and vehicle-treated hearts, respectively, P Ͻ 0.05). Ischemia-mediated protein oxidation was reduced by acetaminophen. The ability of acetaminophen to attenuate the damaging effects of peroxynitrite and hydrogen peroxide and to limit protein oxidation suggest antioxidant mechanisms are responsible for its cardioprotective properties during postischemia-reperfusion. coronary circulation; ventricular function; peroxynitrite; hydrogen peroxide ACETAMINOPHEN WAS INTRODUCED into Western medicine more than 100 years ago, and its pain-relieving and temperature-lowering actions have been under investigation for several decades (1, 9, 26). Potential cardiovascular properties of acetaminophen have gone undiscovered, in part, because no one has made an effort to do the experiments. This might have been influenced by standard textbooks of pharmacology that report that acetaminophen lacks efficacy in the cardiovascular system of mammals (12). The recent literature, however, suggests that investigators are beginning to fill this void. For example, Nakamoto et al. (22) reported beneficial effects of acetaminophen against gastric mucosal injury caused by ischemia-reperfusion in the rat. Farquhar et al. (10) reported reduced renal dysfunction in the stressed human kidney in the presence of acetaminophen vs. ibuprofen, and Colletti et al. (7) fou...

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Section: Hydrogen Peroxide and Acetaminophenmentioning

confidence: 99%

Section: Hydrogen Peroxide and Acetaminophenmentioning

confidence: 99%

mentioning

confidence: 99%

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Acetaminophen and low-flow myocardial ischemia: efficacy and antioxidant mechanisms

Merrill

2002

American Journal of Physiology-Heart and Circulatory Physiology

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“…For d-phenylalanine an elevation to 192 nm was advantageous because the noise could be reduced by selecting a higher bandwidth during data acquisition. Previous studies using HPLC coupled with photometric detection to analyse hydroxylation or nitration products of phenylalanine usually applied a detection wavelength of 274 nm [17,18,[21][22][23][24]. Although these compounds have a relative absorption maximum of around 274 nm (Table 2), all absolute maxima are below 200 nm, with the exception of nitrotyrosine (217 nm).…”

Section: Compoundmentioning

confidence: 98%

Analysis of hydroxylation and nitration products of d-phenylalanine for in vitro and in vivo radical determination using high-performance liquid chromatography and photodiode array detection

Oeckl

Ferger

2009

Journal of Chromatography B

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“…For this reason, we chose to use xanthine oxidase despite the possibility of ⅐OH scavenging by uric acid. Xanthine oxidase has been widely used in free radical research as a means to produce ⅐OH radicals (35,36).…”

Section: Production Of ⅐Ohmentioning

confidence: 99%

An in Vitro Hydroxyl Radical Generation Assay for Microdialysis Sampling Calibration

Chen

Stenken

2002

Analytical Biochemistry

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Detection of Hydroxyl Radicals by -Phenylalanine Hydroxylation: A Specific Assay for Hydroxyl Radical Generation in Biological Systems

Cited by 29 publications

References 35 publications

Acetaminophen and low-flow myocardial ischemia: efficacy and antioxidant mechanisms

Acetaminophen and low-flow myocardial ischemia: efficacy and antioxidant mechanisms

Analysis of hydroxylation and nitration products of d-phenylalanine for in vitro and in vivo radical determination using high-performance liquid chromatography and photodiode array detection

An in Vitro Hydroxyl Radical Generation Assay for Microdialysis Sampling Calibration

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