Detection of hyperdiploid malignant cells in body cavity effusions by fluorescence in Situ hybridization on thinPrep slides

Florentine, Barbara D.; Sanchez, Barry; Raza, Anwar; Frankel, Kenneth A.; Martin, Sue Ellen; Kovacs, Bruce W.; Felix, Juan C.

doi:10.1002/(sici)1097-0142(19971025)81:5<299::aid-cncr8>3.0.co;2-i

Cited by 18 publications

(8 citation statements)

References 7 publications

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“…[25][26][27] Certainly, it is true that ThinPrep processing offers certain advantages over conventional techniques, such as clearing of obscuring background inflammation to allow for more rapid screening and the ability to make multiple slides for purposes of immunocytochemistry and molecular cytology. 28,29 The increased occurrence, however, of poor cellular preservation in ThinPrep-processed slides relative to conventionally prepared slides, observed in the present study, often resulted in a blurring of the nuclear details (Figs. 1, 2) important for the interpretation of specimens such as thyroid and breast FNAs.…”

Section: Discussionmentioning

confidence: 47%

Diagnostic value of liquid-based (Thinprep�) preparations in nongynecologic cases

Nasuti¹,

Tam²,

Gupta³

2001

Diagn. Cytopathol.

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Section: Discussionmentioning

confidence: 47%

Diagnostic value of liquid-based (Thinprep�) preparations in nongynecologic cases

Nasuti¹,

Tam²,

Gupta³

2001

Diagn. Cytopathol.

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“…By using an interphase-FISH technique, specific genetic aberrations have been reported in effusion samples of different patient groups with malignant and non-malignant. 12,[18][19][20][21][22] In current study, in order to detect chromosome aneuploidies in 14 patients with non-malignant and 18 patients with malignant we used an interphase-FISH technique on cytologic specimens. Both malignant and non-malignant patients showed significant chromosomes 9 and 11 aneuploidies when compared with their controls.…”

Section: Discussionmentioning

confidence: 99%

Analysis of chromosomes 9 and 11 aneuploidy frequency in pleural effusions of patients with and without malignancy: By interphase FISH technique

Cora

Acar²,

Ceran³

et al. 2005

Cancer Biology & Therapy

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Fluids of body cavities result in a series of pathophysiological events associated with non-malignant and malignant conditions that lead to the formation of exudative effusion. Diagnosis of effusion from the patients is frequently troublesome for the cytologist because of the differentiation and biological behavior of different cells type in effusion. In the present study, chromosomal aneuploidy status in effusion cells derived from 32 patients including 14 patients with non-malignant and 18 patients with malignant diseases [including malign mesothelioma (n = 6), adeno carcinoma (n = 10), small cell carcinoma (n = 2)] was analyzed by using fluorescence in situ hybridization (FISH) with centromere specific probes for chromosomes 9 and 11. There was significant difference in the incidence of chromosomal 9 and 11 aneuploidies when compared with controls (P = 0.000). However, aneuploidies of chromosomes 9 and 11 in effusion cells from patients with malignant disease had significantly higher than in effusion cells from patients with non-malignant (P = 0.000), suggesting that chromosomes 9 and 11 are frequently involved in the status of disease. The present study indicates that there is a association between chromosomes aneuploidies and pleural effusion cell status. Chromosome aneuploidies in non-malignant group may be an indicator of premalignancy.

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“…20 The morphology of the nuclei was taken into consideration. Around 10 to 100 cells were evaluated.…”

Section: Methodsmentioning

confidence: 99%

“…15 The application of fluorescence in situ hybridisation (FISH) as an adjunct to conventional cytology in diagnosing malignant diseases has been reported. [16][17][18][19][20][21] These studies take advantage of the fact that, although normal cells are usually diploid, many of the common malignant tumours harbour numerical chromosomal abnormalities that can be detected by FISH. Most of these chromosome in situ hybridisation (CISH) studies use fluorescence for visualisation.…”

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confidence: 99%

Chromosome in situ hybridisation, Ki-67, and telomerase immunocytochemistry in liquid based cervical cytology

Cheung

Chiu²,

Tsun³

et al. 2004

J Clin Pathol

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Aims: To assess the potential value of chromosome in situ hybridisation (CISH), Ki-67, and telomerase immunocytochemistry in liquid based cervical cytology to help detect carcinoma cells and precursors. Method: Sixty ThinPrep processed cervical cytology samples were studied: 23 cases within the normal limit, 13 low grade squamous intraepithelial lesions (LSILs), 10 high grade squamous intraepithelial lesions (HSILs), six squamous cell carcinomas, three endocervical adenocarcinomas, two cervical adenosquamous cell carcinomas, and three endometrial adenocarcinomas. CISH was performed with DNA probes specific for the pericentromeric regions of chromosome 11 and 16. Hybridisation signals were visualised with the streptavidin-biotin peroxidase technique. The monoclonal MIB1 and polyclonal TRT-H231 antibodies were used to detect Ki-67 and telomerase immunoreactivity, respectively. Results: Non-specific background staining was almost absent in CISH slides. Normal squamous and glandular cells showed a diploid chromosomal pattern. A relative gain in chromosomes 11 and 16 (aneusomy) was seen in HSIL and the carcinomas (p,0.0001). In MIB1 stained smears, normal cells and koilocytes showed inconspicuous immunoreactivity, whereas strongly immunoreactive nuclei were found in cancer cells and HSIL (p,0.0001). Not only carcinoma and HSIL cells, but also some normal cells, showed cytoplasmic staining for telomerase. Conclusions: These preliminary results indicate that ThinPrep processed cervical smears are suitable for CISH and immunocytochemical studies. The neoplastic squamous and glandular cells were easily identified based on nuclear aneusomy and strong Ki-67 immuoreactivity in the context of abnormal nuclear morphology. This is the first study to apply CISH in cervical cytology using an immunoenzymatic approach. C ervical cancer is one of the most common forms of cancer in women worldwide. Cytological examination of cervical smears is the most widely applied screening method for cervical cancer and its precursors. However, the success of the Papanicolaou smear test is limited with respect to sensitivity and specificity. False negative rates for cervical premalignant lesions and cervical cancer lie between 15% and 50% and false positive rates of approximately 30% have been reported.1 Such suboptimal performance may be related to the subjectivity of cytological diagnosis. ThinPrep processing, an automated cytopreparatory method, has been reported to produce good quality cervical cytology preparations. Moreover, it allows the use of auxiliary laboratory techniques, which may help to distinguish neoplastic from benign diseases, and thus may further improve the efficiency of cancer detection in cervical cytology.A wide array of immunohistochemical and molecular markers have been tested to evaluate their specificity in staining dysplastic cells in cervical smears.2-8 Immunohistochemical detection of the MIB1 (Ki-67) antigen is one of the most frequently used methods for studying cell proliferation in cancer. 9 We have shown in ea...

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Detection of hyperdiploid malignant cells in body cavity effusions by fluorescence in Situ hybridization on thinPrep slides

Cited by 18 publications

References 7 publications

Diagnostic value of liquid-based (Thinprep�) preparations in nongynecologic cases

Diagnostic value of liquid-based (Thinprep�) preparations in nongynecologic cases

Analysis of chromosomes 9 and 11 aneuploidy frequency in pleural effusions of patients with and without malignancy: By interphase FISH technique

Chromosome in situ hybridisation, Ki-67, and telomerase immunocytochemistry in liquid based cervical cytology

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