Detection of<i>Aeromonas hydrophila</i>Using Fiber Optic Microchannel Sensor

Gauri, Samla; Abidin, Zurina Zainal; Kamuri, Mohd Firdaus; Mahdi, Mohd Adzir; Yunus, Nurul Amziah Md

doi:10.1155/2017/8365189

Cited by 13 publications

(11 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In view of the results obtained, A. hydrophila absorbed at the wavelength range of 325-345 nm. These results seem to be consistent with other researchers, which found the wavelength peak for A. hydrophila to be within this wavelength range [31,35].…”

Section: Resultssupporting

confidence: 93%

See 1 more Smart Citation

Performance Evaluation of Free-Space Fibre Optic Detection in a Lab-on-Chip for Microorganism

et al. 2019

Self Cite

View full text Add to dashboard Cite

This paper describes the development of a lab-on-chip (LOC) device that can perform reliable online detection in continuous-flow systems for microorganisms. The objective of this work was to examine the performance of a fibre optic detection system integrated into a LOC device. The microfluidic system was fabricated using dry film resist (DFR), integrated with multimode fibre pigtails in the LOC. Subsequently, the performance of the fibre optic detection was evaluated by its absorbance spectra, detection limit, repeatability and reproducibility, and response time. The analysis was carried out using a constant flow rate for three different types of microorganisms which are Escherichia coli, Saccharomyces cerevisiae, and Aeromonas hydrophila. Under the experimental conditions used in this study, the detection limit of 1.0×105 cells/mL for both A. hydrophila and E. coli, while a detection limit of 1.0×106 cells/mL for S. cerevisiae cells were measured. The results also revealed that the device showed good repeatability with standard deviations less than 0.2 for A. hydrophila and E. coli, while standard deviations for S. cerevisiae were larger than 1.0. The response times for A. hydrophila, E. coli, and S. cerevisiae were 104 s, 122 s, and 78 s, respectively, although significant errors were recorded for all three species for reproducibility experiment. It was found that the device showed generally good sensitivity, with the highest sensitivity towards S. cerevisiae. These findings suggest that an integrated LOC device, with embedded multimode fibre pigtails, can be a reliable instrument for microorganism detection.

show abstract

Section: Resultssupporting

confidence: 93%

“…Fabrication of LOC Devices. Fabrication of microfluidic integrated with fibre optic was based on already reported procedures using dry film resist [30,31]. The design of the microfluidic channel is shown in Figure 1.…”

Section: Methodsmentioning

confidence: 99%

Performance Evaluation of Free-Space Fibre Optic Detection in a Lab-on-Chip for Microorganism

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…The microfluidic channel was fabricated on a glass substrate using dry film resist (DFR) (Ordyl Alpha 940) by soft lithography. The microfluidic fabrication was prepared according to the procedure used by [36,37]. For the patterning processes, microstructures were designed in the Autocad program and fabricated on a negative mask (Figure 2).…”

Section: Methodsmentioning

confidence: 99%

Separation and Detection of Escherichia coli and Saccharomyces cerevisiae Using a Microfluidic Device Integrated with an Optical Fibre

et al. 2019

View full text Add to dashboard Cite

This paper describes the development of an integrated system using a dry film resistant (DFR) microfluidic channel consisting of pulsed field dielectrophoretic field-flow-fractionation (DEP-FFF) separation and optical detection. The prototype chip employs the pulse DEP-FFF concept to separate the cells (Escherichia coli and Saccharomyces cerevisiae) from a continuous flow, and the rate of release of the cells was measured. The separation experiments were conducted by changing the pulsing time over a pulsing time range of 2–24 s and a flow rate range of 1.2–9.6 μ L min − 1 . The frequency and voltage were set to a constant value of 1 M Hz and 14 V pk-pk, respectively. After cell sorting, the particles pass the optical fibre, and the incident light is scattered (or absorbed), thus, reducing the intensity of the transmitted light. The change in light level is measured by a spectrophotometer and recorded as an absorbance spectrum. The results revealed that, generally, the flow rate and pulsing time influenced the separation of E. coli and S. cerevisiae. It was found that E. coli had the highest rate of release, followed by S. cerevisiae. In this investigation, the developed integrated chip-in-a lab has enabled two microorganisms of different cell dielectric properties and particle size to be separated and subsequently detected using unique optical properties. Optimum separation between these two microorganisms could be obtained using a longer pulsing time of 12 s and a faster flow rate of 9.6 μ L min − 1 at a constant frequency, voltage, and a low conductivity.

show abstract

“…For complete of discussion, Table 2 25‐206 summarized all of the discussed articles on the detection of bacterial. It is important to point out that, there is other reports in this research area …”

Section: Biosensing Based On Type Of Bacteriamentioning

confidence: 99%

Recent progress and challenges on the bioassay of pathogenic bacteria

2020

View full text Add to dashboard Cite

The present review (containing 242 references) illustrates the importance and application of optical and electrochemical methods as well as their performance improvement using various methods for the detection of pathogenic bacteria. The application of advanced nanomaterials including hyper branched nanopolymers, carbon‐based materials and silver, gold and so on. nanoparticles for biosensing of pathogenic bacteria was also investigated. In addition, a summary of the applications of nanoparticle‐based electrochemical biosensors for the identification of pathogenic bacteria has been provided and their advantages, detriments and future development capabilities was argued. Therefore, the main focus in the present review is to investigate the role of nanomaterials in the development of biosensors for the detection of pathogenic bacteria. In addition, type of nanoparticles, analytes, methods of detection and injection, sensitivity, matrix and method of tagging are also argued in detail. As a result, we have collected electrochemical and optical biosensors designed to detect pathogenic bacteria, and argued outstanding features, research opportunities, potential and prospects for their development, according to recently published research articles.

show abstract

Detection ofAeromonas hydrophilaUsing Fiber Optic Microchannel Sensor

Cited by 13 publications

References 42 publications

Performance Evaluation of Free-Space Fibre Optic Detection in a Lab-on-Chip for Microorganism

Performance Evaluation of Free-Space Fibre Optic Detection in a Lab-on-Chip for Microorganism

Separation and Detection of Escherichia coli and Saccharomyces cerevisiae Using a Microfluidic Device Integrated with an Optical Fibre

Recent progress and challenges on the bioassay of pathogenic bacteria

Contact Info

Product

Resources

About