Detection of<i>Brucella</i>antibodies in domestic animals of southern Cameroon: Implications for the control of brucellosis

Kamga, Rolin M. N.; Silatsa, Barberine A.; Farikou, Oumarou; Kuiaté, Jules-Roger; Simo, Gustave

doi:10.1002/vms3.264

Cited by 17 publications

(43 citation statements)

References 45 publications

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“…suis , [ 14 ]. In 2020, small ruminants and pigs were shown to be infected with a smooth strain Brucella spp., but the specific species remains unknown [ 26 ]. It is clear that there is a disease burden, but a continuous surveillance system utilizing standard diagnostic techniques is necessary to adequately define the current status of disease in both animals and humans.…”

Section: Discussionmentioning

confidence: 99%

“…Of these, nine of the studies assessed only cattle, one evaluated only human subjects, and one investigated both cattle and humans. Most recently, for the first time in this country, disease prevalence in small ruminants, swine, and dogs has been investigated [26]. This research was split between semi-intensive and pastoralist communities [26].…”

Section: Plos Onementioning

confidence: 99%

“…Most recently, for the first time in this country, disease prevalence in small ruminants, swine, and dogs has been investigated [26]. This research was split between semi-intensive and pastoralist communities [26]. Unfortunately, the study grouped all of the different animal species together, from all of the dispersed geographic locations (tropical forest, mountains, and central plateau), in its final seroprevalence estimate, making it difficult to analyze and draw conclusions from the data [26].…”

Section: Plos Onementioning

confidence: 99%

“…This research was split between semi-intensive and pastoralist communities [26]. Unfortunately, the study grouped all of the different animal species together, from all of the dispersed geographic locations (tropical forest, mountains, and central plateau), in its final seroprevalence estimate, making it difficult to analyze and draw conclusions from the data [26]. Despite wildlife also being an important factor for this country, due to transhumance and extensive production systems, no studies have ever been conducted in these species.…”

Section: Plos Onementioning

confidence: 99%

“…However, it is important to highlight the discrepancy in the usage of the standard serological testing approach between studies and the interpretation of such. Represented in Table 1, five of the studies used the Rose Bengal Test (RBT) in combination with the indirect enzyme-linked immunosorbent assay (iELISA) [18,[24][25][26][27], and one used RBT in combination with the complement fixation test (CFT) for confirmation [17]. In contrast, two studies simply employed the RBT [16,23], and the remaining four relied solely on the competitive ELISA (C-ELISA) [19][20][21][22].…”

Section: Plos Onementioning

confidence: 99%

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Scoping review of brucellosis in Cameroon: Where do we stand, and where are we going?

et al. 2020

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Brucellosis is a zoonotic disease known to be endemic to parts of western and sub-Saharan Africa. However, the epidemiology for humans and animals remains largely unknown in many of these countries with Cameroon being a typical example. Despite common knowledge that brucellosis affects livestock, the actual number of infected animals remains unknown. Through a scoping review, the current known status of the disease is described. The aim is to ascertain relevant and publicly accessible research and knowledge of human and animal brucellosis in the country, and to provide an overview of the factors associated with its known persistence. Seroprevalence has been estimated and published in 12 separate instances (1 human; 9 cattle; 1 human and cattle; and 1 that includes cattle, pigs, and small ruminants), between 1982 and 2020, in 9 of the country's 10 geopolitical regions. In 1983, Brucella abortus and B. melitensis were isolated in cattle, but no further bacterial isolation has been published since. The seroprevalence from 196 total humans has ranged between 5.6% and 28.1%, and between 3.0% and 30.8% for 14,044 total cattle. As there is no ongoing surveillance program, it is not currently possible to identify the specific Brucella spp. that are endemic to the country and its regions. There are sufficient agricultural systems of cattle, pigs, goats, and sheep to sustain the presence of multiple Brucella spp. Surveillance information is the cornerstone of epidemiologic decision making, and is needed to direct policy makers, public health authorities, and veterinary services to appropriate actions. A combination of serological and molecular based diagnostics for surveillance is necessary to identify, quantify, and direct the appropriate public health interventions. Cameroon has an opportunity to build public and animal health infrastructure, leading the way for central Africa in the management and future eradication of brucellosis.

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Section: Discussionmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

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Scoping review of brucellosis in Cameroon: Where do we stand, and where are we going?

et al. 2020

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Potential Biomarkers for Neurobrucellosis Diagnosis and Treatment Based on Cerebrospinal Fluid Metabolomics Analysis

Liu,

Shi,

Guo

et al. 2024

Rapid Comm Mass Spectrometry

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BackgroundZhenzhu Tongluo pills (ZZTL) have been utilized for the treatment of neurobrucellosis (NB) in the Inner Mongolia region of China. However, the specific mechanism underlying the neuroprotective effects of ZZTL in NB remains insufficiently explored. Therefore, this study aimed to analyze the metabolite profiles across different groups to identify potential biomarkers for the diagnosis and treatment of NB.MethodsLC–MS analysis was used to screen differential metabolites in cerebrospinal fluid (CSF) samples from control subjects, NB patients, and NB patients undergoing ZZTL therapy.ResultsWe identified 225 common metabolites across two comparison groups (NB vs. control group and NB + ZZTL vs. NB group). Among these metabolites, 138 were downregulated, and 87 were upregulated in the NB group compared to the control group, while the levels of these metabolites were reversed by ZZTL therapy. Creatinine exhibited the highest VIP score in both comparison groups, with elevated levels observed in CSF samples from NB patients compared to controls; however, its levels were reduced following ZZTL therapy. Additionally, hypoxanthine and uric acids levels were also increased in CSF samples from NB patients, indicating dysregulation of purine metabolism in NB; however, these changes were reversed by ZZTL. Furthermore, pantothenic acid (vitamin B5) and niacinamide (vitamin B3) levels were decreased in CSF samples from NB patients, suggesting a potential link between NB and vitamin B3 and B5 deficiency; however, these changes were reversed by ZZTL.ConclusionCollectively, CSF metabolomics may effectively differentiate NB patients from control subjects, providing valuable insights for NB diagnosis and treatment.

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Seroprevalence study of brucellosis in wild boar hunted for private consumption in northeast Portugal

Ruano,

Mateus,

Chorense

et al. 2024

Vet Res Commun

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Brucellosis is an important infectious disease caused by bacteria of the genus Brucella. In the northeast region of Portugal, infection with Brucella melitensis is endemic in small ruminants, and there are also humans’ cases. However, the epidemiological role of the wild boar in the dynamics of this disease in this region is unknown. In this study, a total of 332 blood samples were collected from wild boar hunted in thirty-six hunting areas during the 2022/2023 hunting season. All were taken by the hunters for private consumption, with no evisceration or examination in the field. Serum samples were tested by indirect ELISA (i-ELISA). It was observed that 88 wild boars were exposed to Brucella spp., pointing to a seroprevalence of 26.5% (95% CI: 21.8 – 31.3%). This high prevalence underlines the importance that wild boar may have in the dynamics of this disease in the region and its potential transmission to other animals, and to humans (for example, during the handling of carcasses). Increased awareness and knowledge of brucellosis in wild boar is essential for the implementation of effective practices and habits and, consequently, for the control and prevention of this important zoonosis.

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Detection ofBrucellaantibodies in domestic animals of southern Cameroon: Implications for the control of brucellosis

Cited by 17 publications

References 45 publications

Scoping review of brucellosis in Cameroon: Where do we stand, and where are we going?

Scoping review of brucellosis in Cameroon: Where do we stand, and where are we going?

Potential Biomarkers for Neurobrucellosis Diagnosis and Treatment Based on Cerebrospinal Fluid Metabolomics Analysis

Seroprevalence study of brucellosis in wild boar hunted for private consumption in northeast Portugal

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