Detection of<i> Enterocytozoon bieneusi</i> in White Yaks in Gansu Province, China

Ma, Jian-Gang; Zhang, Nian‐Zhang; Hou, Junling; Zou, Yang; Gui-xue, HU; Zhu, Xing‐Quan; Zhou, Dong‐Hui

doi:10.1155/2017/5790181

Cited by 15 publications

(10 citation statements)

References 19 publications

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“…We determined the prevalence and genetic diversity of E . bieneusi among yaks in five districts of Tibet, and the infection rate (5.0%, 29/577) was higher than previously reported in one study in Gansu (1.1%, 4/353) [16], but lower than that of two studies in Qinghai (7.0%, 40/554 and 7.2%, 40/554) [15, 17].…”

Section: Discussionmentioning

confidence: 61%

Molecular characterization and distribution of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi from yaks in Tibet, China

Chang

Zhang

et al. 2019

BMC Vet Res

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BackgroundWith worldwide distribution and importance for veterinary medicine, Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi have been found in a wide variety of vertebrate hosts. At present, few available molecular data can be used to understand the features of genetic diversity of these pathogens in areas without or less intensive farming. Dominated by grazing, Tibet is a separate geographic unit in China and yaks are in frequent contact with local herdsmen and necessary for their daily life. Therefore, to investigate the distribution of these pathogens in yaks of Tibet, 577 fecal specimens were screened using nested PCR for the presence and genotypes of the three intestinal pathogens.ResultsThe overall prevalence of Cryptosporidium spp., G. duodenalis, and E. bieneusi were 1.4% (8/577), 1.7% (10/577), and 5.0% (29/577), respectively. Cryptosporidium andersoni (n = 7) and Cryptosporidium bovis (n = 1) were detected by sequence analysis of the SSU rRNA gene. Genotyping at the SSU rRNA and triosephosphate isomerase genes suggested that all G. duodenalis positive specimens belonged to assemblage E. Sequence analysis of the internal transcribed spacer gene identified six known E. bieneusi genotypes: BEB4 (n = 11), I (n = 6), D (n = 5), J (n = 2), CHC8 (n = 1), and BEB6 (n = 1). One subtype (A5,A4,A2,A1) for C. andersoni and three multilocus genotypes for E. bieneusi were identified by multilocus sequence typing.ConclusionsWe report for the first time the status of three enteric pathogens infection simultaneously for grazing yaks in Tibet. Yaks in our study are likely to impose a low zoonotic risk for humans. The molecular epidemiology data add to our knowledge of the characteristics of distribution and transmission for these pathogens in Tibet and their zoonotic potential and public health significance.

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Section: Discussionmentioning

confidence: 61%

Molecular characterization and distribution of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi from yaks in Tibet, China

Chang

Zhang

et al. 2019

BMC Vet Res

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“…In China, the average prevalence of E. bieneusi in animals ranges from 0.9% (4/426) in rabbits [ 12 ] to 45.6% (426/934) in pigs [ 13 ]. However, E. bieneusi infection has only been reported in some animals in northwestern China (Table 2 ), the average prevalence ranging from 1.1% (4/353) in white yaks [ 14 ] to 47.8% (22/46) in sheep [ 2 ]. In Xinjiang, only dairy calves [ 15 ] and grazing horses [ 16 ] have been previously reported to have E. bieneusi infections, with a prevalence of 16.5% (85/514) and 30.9% (81/262), respectively.…”

Section: Resultsmentioning

confidence: 99%

“…infected (%) Genotype (no.) Reference Xinjiang Dairy cattle 514 85 (16.5) J (57), I (19), BEB4 (4), D (2), EbpC (2), CC4 (1) [ 15 ] Xinjiang Grazing horses 262 81 (30.9) EbpC (21), EpbA (20), BEB6 (9), CHG19 (2), CM6 (4), CM7 (2), CM8 (1), CS-1 (1), CS-4 (1), D (1), G (3), horse1 (4), horse2 (2), O (4), Peru8 (1), XJH1 (2), XJH2 (1), XJH3 (1), XJH4 (1) [ 16 ] Gansu White yaks 353 4 (1.1) BEB4 (2), I (1), WCY1 (1) [ 14 ] Ningxia Dairy cattle 109 51 (46.8) J (25), CM8 (14), I (8), O (1), CHC1 (1), CHC2 (1), CHC3 (1) [ 6 ] Qinghai Yaks 327 23 (7.0) BEB4 (16), I (1), J (1), CHN11 (4), CHN12 (1) [ 18 ] Shaanxi Sheep 46 22 (47.8) BEB6 (4), CHG1 (3), CHG3 (3), CD6 (3), CHG5 (2), E (1), F (1), CHG14 (1), CHG16 (1), CHG24 (1) [ 2 ] Shaanxi Monkeys 197 25 (12.7) D (10), BEB6 (4), MH (7), XH (2), BSH (2) [ 19 ] Shaanxi Dairy cattle 198 39 (19.7) I (21), J (16), CHN1 (1), CSX1 (1) [ 20 ] Shaanxi Beef cattle 173 34 (19.7) I (19), J (14), CSX2 (1) [ 20 ] Shaanxi Golden takins 191 28 (14.7) BEB6 (10), D (8), I (6), TEB1–4 (each 1) [ 21 ] Shaanxi Cashmere goats 315 50 (15.9) S...…”

Section: Resultsmentioning

confidence: 99%

Host specificity of Enterocytozoon bieneusi genotypes in Bactrian camels (Camelus bactrianus) in China

Zhao

et al. 2018

Parasites Vectors

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BackgroundEnterocytozoon bieneusi is an obligate, intracellular fungus and is commonly reported in humans and animals. To date, there have been no reports of E. bieneusi infections in Bactrian camels (Camelus bactrianus). The present study was conducted to understand the occurrence and molecular characteristics of E. bieneusi in Bactrian camels in China.ResultsOf 407 individual Bactrian camel fecal specimens, 30.0% (122) were E. bieneusi-positive by nested polymerase chain reaction (PCR) based on internal transcriber spacer (ITS) sequence analysis. A total of 14 distinct E. bieneusi ITS genotypes were obtained: eight known genotypes (genotype EbpC, EbpA, Henan-IV, BEB6, CM8, CHG16, O and WL17), and six novel genotypes (named CAM1 to CAM6). Genotype CAM1 (59.0%, 72/122) was the most predominant genotype in Bactrian camels in Xinjiang, and genotype EbpC (18.9%, 23/122) was the second-most predominant genotype. Phylogenetic analysis revealed that six known genotypes (EbpC, EbpA, WL17, Henan-IV, CM8 and O) and three novel genotypes (CAM3, CAM5 and CAM6) fell into the human-pathogenic group 1. Two known genotypes (CHG16 and BEB6) fell into the cattle host-specific group 2. The novel genotypes CAM1, CAM 2 and CAM4 cluster into group 8.ConclusionsTo our knowledge, this is the first report of E. bieneusi in Bactrian camels. The host-specific genotype CAM1 was the predominant genotype, which plays a negligible role in the zoonotic transmission of E. bieneusi. However, the second-most predominant genotype, EbpC, has greater zoonotic potential.

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“…The first cases of E. bieneusi infection in cattle were found in Germany, and two genotypes, I and J, were identified [ 12 ]. The prevalence of E. bieneusi in cattle ranged from 1.13% to 46.8% in China, the United States, Argentina, South Korea, Germany, Portugal, South Africa, Brazil, and the Czech Republic [ 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ]. Although most of E. bieneusi genotypes are detected in cattle and belong to Group 2, these genotypes have also been identified in humans, animals, drinking water sources, and municipal sewage, indicating the possibility of cross-species or zoonotic E. bieneusi transmission [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Novel Genotypes Identification of Enterocytozoon bieneusi in Dairy Cattle in Yunnan Province, China

Song

Wang

Yang

et al. 2021

Animals

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Enterocytozoon bieneusi is a fungus-like protist parasite that can cause diarrhea and enteric diseases. The infection of E. bieneusi has been reported in many host species, including cattle and humans. However, information on prevalence and genotype distribution of E. bieneusi in dairy cattle in Yunnan province in China is still absent. In this study, 490 Holstein Cows and 351 dairy buffalo fecal samples were collected from three regions in Yunnan province, China. By using nest-PCR that targets the internal transcribed spacer (ITS), we found that the prevalence of E. bieneusi was 0.59% (5/841). DNA sequence analysis showed that five E. bieneusi genotypes were identified in this study, including two novel genotypes, YNDCEB-90 and YNDCEB-174, and three known genotypes (I, J, BEB4). Phylogenetic analysis revealed that two novel genotypes, YNDCEB-90 and YNDCEB-174, were clustered into Group 1, representing the zoonotic potential. The remaining genotypes I, J, and BEB4, which are the most frequent genotypes of E. bieneusi infection in cattle and lead to E. bieneusi infection in humans, belonged to Group 2. Although the lower prevalence of E. bieneusi was detected in dairy cattle in Yunnan province, it indicates that dairy cattle should be considered to be one of the potential hosts for transmitting E. bieneusi to humans. These findings are important for the development of effective prevention strategies for microsporidiosis.

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Detection of Enterocytozoon bieneusi in White Yaks in Gansu Province, China

Cited by 15 publications

References 19 publications

Molecular characterization and distribution of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi from yaks in Tibet, China

Molecular characterization and distribution of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi from yaks in Tibet, China

Host specificity of Enterocytozoon bieneusi genotypes in Bactrian camels (Camelus bactrianus) in China

Prevalence and Novel Genotypes Identification of Enterocytozoon bieneusi in Dairy Cattle in Yunnan Province, China

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