Detection ofEscherichia coliO157 andEscherichia coliO157:H7 by the immunomagnetic separation technique andstx1andstx2genes by multiplex PCR in slaughtered cattle in Samsun Province, Turkey

Abstract

This study was conducted to investigate the presence of Escherichia (E.) coli O157 and E. coli O157:H7 and stx1 and stx2 genes on cattle carcasses and in rectal samples collected from Samsun Province of Turkey. A total of 200 samples collected from cattle carcasses and the rectal contents of 100 slaughtered cattle from two commercial abattoirs were tested using the immunomagnetic separation technique and multiplex PCR methods. E. coli O157 and E. coli O157:H7 were detected in 52 of the 200 samples (26%) tested. Of the positive samples, 49 were E. coli O157 and three were E. coli O157:H7. The E. coli O157 strain was isolated from 24 carcasses and 25 rectal samples, while E. coli O157:H7 was isolated from two carcasses and one rectal sample. Of the 49 samples positive for E. coli O157, 32 were from the rectal and carcass samples of the same animal, while two E. coli O157:H7 isolates were obtained from rectal swabs and carcasses of the same animal. The stx1 and stx2 genes were both detected in 35 E. coli O157 isolates and one E. coli O157:H7 isolate, but the stx2 gene was only detected alone in two E. coli O157 isolates. Overall, 16 carcasses tested positive for E. coli O157 and one carcass tested positive for E. coli O157:H7 based on both carcass and rectal samples. Overall, the results of this study indicate that cattle carcasses pose a potential risk to human health due to contamination by E. coli O157 and E. coli O157:H7 in the feces.

Elazığ’da Mezbahalarda Kesilen Sığırlardan ve Piyasada Satılan Kıymalardan Shiga Toksin Üreten Escherichia coli O157’nin İzolasyonu, Virulens Genleri ve Antibiyotiklere Duyarlılıkları

“…However, in some of these studies the presence of shiga toxin producing E. coli O157 was examined. In the study by Inat and Siriken 13 conducted in Samsun city, the authors reported they had isolated STEC O157 from 18% of the rectal swab samples taken from 100 slaughtered cattle. In the study conducted by Aslantas et al 11 in Hatay city, it was reported that in 11% of 565 cattle faecal samples, STEC O157 was detected.…”

“…In studies conducted in several regions of Turkey, it is reported that E. coli O157 was detected in cattle faeces with rates varying between 0.6% and 25% [10][11][12][13] . However, in some of these studies the presence of shiga toxin producing E. coli O157 was examined.…”

“…Antibiotic resistance strains of STEC have been reported in many countries [7][8][9] . The presence of E. coli O157 in cattle [10][11][12][13] , ground beef [14][15][16][17] and red meat samples 18 has previously been reported in Turkey.…”

Prevalence and Characterization of Shiga Toxin-Producing Escherichia coli Isolated from Slaughtered Qurban Animal in Jakarta Province

“…All the extracted DNA of the standard strains and of the sample isolates were examined using multiplex PCR for molecular typing of the virulence genes (rfbE, stx1, stx2, fliC) using spesific nucleotide primers are listed in Table 1 (Hu et al, 1999). Amplification condition was carried out according to Inat and Siriken (2010) with a modification. The reaction mixture consisted of 4.8 µL of aquadest (DNAse, RNAse free), 10 µL of Maxima hot start green PCR master mix (2x) (Thermo Scientific™), and 0.4 µL of each primer.…”

“…Therefore, at present no information is available about pathogenic bacteria, especially STEC contamination in Qurban festival. Cattle are the main reservoir of STEC, where the organism typically colonizes the lower gastrointestinal tract and shed the bacteria through their feces (Inat & Siriken, 2010;Perera et al, 2015). Also, cattle has a significant proportion of beef become contaminated during slaughter (Kundu et al, 2014).…”