Detection of <i>Gallibacterium anatis</i> by TaqMan fluorescent quantitative PCR

Huangfu, Heping; Xu, Wenbo; Wang, Hongkui; Dong, Qing; Guo, H. W.; Sun, Yuhua; Li, Yunxia; Gao, Wenwen; Wang, Wuqing; Jia, Zhang; Shi, Jingke; Pan, Haochun; Li, Congcong; Wang, Linkang

doi:10.1080/03079457.2017.1416590

Cited by 13 publications

(11 citation statements)

References 20 publications

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“…Real‐time fluorescence qPCR is a commonly used and reliable detection method for nucleic acids. There are 2 approaches that use qPCR: 1 based on fluorescent dyes and 1 that is targeted at specific fluorescent‐labeled DNA sequences, called probes . The invention of TaqMan probes has overcome the limitations of SYBR Green I, that is, its lack of specificity owing to its ability to bind to all DNA double‐stranded structures .…”

Section: Discussionmentioning

confidence: 99%

“…There are 2 approaches that use qPCR: 1 based on fluorescent dyes and 1 that is targeted at specific fluorescent-labeled DNA sequences, called probes. [60][61][62] The invention of TaqMan probes has overcome the limitations of SYBR Green I, that is, its lack of specificity owing to its ability to bind to all DNA double-stranded structures. [63][64][65][66] During the extension stage of TaqMan-PCR, the DNA polymerase removes the TaqMan probe by hydrolysis, resulting in a fluorescent signal.…”

Section: Discussionmentioning

confidence: 99%

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circMAN1A2 could serve as a novel serum biomarker for malignant tumors

et al. 2019

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Novel diagnostic and prognostic biomarkers of cancers are needed to improve precision medicine. Circular RNAs act as important regulators in cancers at the transcriptional and posttranscriptional levels. The circular RNA circMAN1A2 is highly expressed in nasopharyngeal carcinoma according to our previous RNA sequencing data; however, the expression and functions of circMAN1A2 in cancers are still obscure. Therefore, in this study, we evaluated the expression of circMAN1A2 in the sera of patients with nasopharyngeal carcinoma and other malignant tumors and analyzed its correlations with clinical features and diagnostic values. The expression levels of circMAN1A2 were detected by quantitative real‐time PCR, and the correlations of clinical features with circMAN1A2 expression were analyzed by χ2 tests. Receiver operating characteristic curves were used to evaluate the clinical applications of circMAN1A2. The results showed that circMAN1A2 was upregulated in nasopharyngeal carcinoma, oral cancer, thyroid cancer, ovarian cancer, and lung cancer, with areas under the curves of 0.911, 0.779, 0.734, 0.694, and 0.645, respectively, indicating the good diagnostic value of circMAN1A2. Overall, our findings suggested that circMAN1A2 could be a serum biomarker for malignant tumors, providing important insights into diagnostic approaches for malignant tumors. Further studies are needed to elucidate the mechanisms of circMAN1A2 in the pathogenesis of cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

circMAN1A2 could serve as a novel serum biomarker for malignant tumors

et al. 2019

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“…It has a detection rate of 97% compared with that of conventional PCR (78%) and culture (34%). Another qPCR developed by Huangfu et al (2018) that targets gtxA gene showed a better detection rate than qPCR based on gyrB gene. In general, qPCR needs lower concentration of DNA template and in addition to that it takes less time and is cost effective as compared to conventional PCR and phenotypic identification (Wang et al 2016).…”

Section: Real-time Quantitative Pcr (Qpcr)mentioning

confidence: 99%

Etiology, epidemiology, pathology, and advances in diagnosis, vaccine development, and treatment ofGallibacterium anatisinfection in poultry: a review

Krishnegowda

Dhama

Kumar

et al. 2020

Veterinary Quarterly

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Gallibacterium anatis is a Gram-negative bacterium of the Pasteurellaceae family that resides normally in the respiratory and reproductive tracts in poultry. It is a major cause of oophoritis, salpingitis, and peritonitis, decreases egg production and mortality in hens thereby severely affecting animal welfare and overall productivity by poultry industries across Europe, Asia, America, and Africa. In addition, it has the ability to infect wider host range including domesticated and free-ranging avian hosts as well as mammalian hosts such as cattle, pigs and human. Evaluating the common virulence factors including outer membrane vesicles, fimbriae, capsule, metalloproteases, biofilm formation, hemagglutinin, and determining novel factors such as the RTX-like toxin GtxA, elongation factor-Tu, and clustered regularly interspaced short palindromic repeats (CRISPR) has pathobiological, diagnostic, prophylactic, and therapeutic significance. Treating this bacterial pathogen with traditional antimicrobial drugs is discouraged owing to the emergence of widespread multidrug resistance, whereas the efficacy of preventing this disease by classical vaccines is limited due to its antigenic diversity. It will be necessary to acquire in-depth knowledge on important virulence factors, pathogenesis and, concerns of rising antibiotic resistance, improvised treatment regimes, and novel vaccine candidates to effectively tackle this pathogen. This review substantially describes the etio-epidemiological aspects of G. anatis infection in poultry, and updates the recent development in understanding the pathogenesis, organism evolution and therapeutic and prophylactic approaches to counter G. anatis infection for safeguarding the welfare and health of poultry.

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“…The description that LOC284454 is significantly reduced in prostate, uterus, breast, and kidney cancer [44] suggests instead that LOC284454 is relatively specific and is highly expressed in HNC. [49][50][51]. Since the qPCR instrument has a multicolor fluorescent channel, the experimental group and the control group are allowed to react in the same tube with the same cDNA template, which can reduce systematic errors and improve the specificity and sensitivity of the experiment [52].…”

Section: Discussionmentioning

confidence: 99%

Upregulation of long non-coding RNA LOC284454 may serve as a new serum biomarker for head and neck cancers

Fan¹,

Wang²,

Tang³

et al. 2020

Preprint

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Purpose: Identification of effective diagnostic and prognostic biomarkers of cancer is necessary for improving precision medicine. Long non-coding RNAs(lncRNAs) play an important regulatory role in tumorinitiation and progression. The lncRNA LOC284454 is distinctly expressed in various head and neck cancers(HNCs), as demonstrated by our previous bioinformatics analysis. However, the expression levels and functions of LOC284454 in cancer are still unclear.Methods: We investigated the dysregulation of lncRNAs in HNCs using the GEO database and found that LOC284454 was highly expressed in HNCs. Serum samples from 212 patients with HNCs and 121 normal controls were included in this biomarker study. We measured the expression of LOC284454 in the sera of HNC patients and normal controls using RT-qPCR. Receiver operating characteristics (ROC) analysis is an important statistical method that is widely used in clinical diagnosis and population screening. ROC was used to analyze the clinical value of LOC284454 in the early diagnosis of HNCs.Results: LOC284454 was significantly upregulated in the sera of patients with nasopharyngeal carcinoma, oral cancer, and thyroid cancer. LOC284454 upregulation had good clinical diagnostic value in these cancers, as evaluated by area under the ROC curve values of 0.931, 0.698, and 0.834,respectively.Conclusions: LOC284454 may be a valuable serum biomarker forHNCsfacilitating the early diagnosis of malignant cancers.Further studies are needed to elucidate the mechanisms underlying the involvement of LOC284454 in HNCs.This study provides the first evidence that LOC284454 may be a serum biomarker for HNCs.

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Detection of Gallibacterium anatis by TaqMan fluorescent quantitative PCR

Cited by 13 publications

References 20 publications

circMAN1A2 could serve as a novel serum biomarker for malignant tumors

circMAN1A2 could serve as a novel serum biomarker for malignant tumors

Etiology, epidemiology, pathology, and advances in diagnosis, vaccine development, and treatment ofGallibacterium anatisinfection in poultry: a review

Upregulation of long non-coding RNA LOC284454 may serve as a new serum biomarker for head and neck cancers

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