Detection of<i>inv</i>A gene of<i>Salmonella</i>by DNA-gold nanoparticles biosensor and its comparison with PCR

Majdinasab, Marjan; Aminlari, Mahmoud; Sheikhi, Mohammad Hossein; Niakousari, Mehrdad; Shekarforoosh, Shahram

doi:10.1080/17458080.2011.569575

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…AuNPs possess unique optical properties with color shifts corresponding to their dispersion and aggregation status that well‐dispersed AuNPs solution displays a red color and the aggregated one appears in purple or blue (Bui, Ahmed, & Abbas, 2015). Based on DNA hybridization, AuNPs‐based methods are commonly used for the detection of Salmonella genomic DNA (Majdinasab, Aminlari, Sheikhi, Niakousari, & Shekarforoosh, 2013; Prasad, Shankaracharya, & Vidyarthi, 2011; Thavanathan, Huang, & Thong, 2015). For example, Thavanathan et al.…”

Section: Biosensors With Different Transduces For Detection Of Salmonmentioning

confidence: 99%

Biosensors for rapid detection of Salmonella in food: A review

Shen

2020

Comp Rev Food Sci Food Safe

148

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Salmonella is one of the main causes of foodborne infectious diseases, posing a serious threat to public health. It can enter the food supply chain at various stages of production, processing, distribution, and marketing. High prevalence of Salmonella necessitates efficient and effective approaches for its identification, detection, and monitoring at an early stage. Because conventional methods based on plate counting and real‐time polymerase chain reaction are time‐consuming and laborious, novel rapid detection methods are urgently needed for in‐field and on‐line applications. Biosensors provide many advantages over conventional laboratory assays in terms of sensitivity, specificity, and accuracy, and show superiority in rapid response and potential portability. They are now recognized as promising alternative tools and one of the most on‐site applicable and end user–accessible methods for rapid detection. In recent years, we have witnessed a flourishing of studies in the development of robust and elaborate biosensors for detection of Salmonella in food. This review aims to provide a comprehensive overview on Salmonella biosensors by highlighting different signal‐transducing mechanisms (optical, electrochemical, piezoelectric, etc.) and critically analyzing its recent trends, particularly in combination with nanomaterials, microfluidics, portable instruments, and smartphones. Furthermore, current challenges are emphasized and future perspectives are discussed.

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Section: Biosensors With Different Transduces For Detection Of Salmonmentioning

confidence: 99%

Biosensors for rapid detection of Salmonella in food: A review

Shen

2020

Comp Rev Food Sci Food Safe

148

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“…In this study, both genomic and amplified DNA sequences were used. The results demonstrated that the efficiency and sensitivity of detection of the AuNP-based assay was relatively low when non-amplified target DNA was used, as compared to Polymerase Chain Reaction (PCR) amplified DNA samples [108]. In a similar study, several pathovars of Pseudomonas syringae bacteria (the causal agent of different agricultural diseases) were detected using two thiol probes attached to AuNPs via cross-linked hybridization.…”

Section: - Colorimetric Detection Based On Aunpsmentioning

confidence: 99%

Optical assays based on colloidal inorganic nanoparticles

Ghasemi

Rabiee

Ahmadi

et al. 2018

Analyst

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Colloidal inorganic nanoparticles have wide applications in the detection of analytes and in biological assays. A large number of these assays rely on the ability of gold nanoparticles (AuNPs, in the 20 nm diameter size range) to undergo a color change from red to blue upon aggregation. AuNP assays can be based on cross-linking, non-cross linking or unmodified charge-based aggregation. Nucleic acid-based probes, monoclonal antibodies, and molecular-affinity agents can be attached by covalent or non-covalent means. Surface plasmon resonance and SERS techniques can be utilized. Silver NPs also have attractive optical properties (higher extinction coefficient). Combinations of AuNPs and AgNPs in nanocomposites can have additional advantages. Magnetic NPs and ZnO, TiO and ZnS as well as insulator NPs including SiO can be employed in colorimetric assays, and some can act as peroxidase mimics in catalytic applications. This review covers the synthesis and stabilization of inorganic NPs and their diverse applications in colorimetric and optical assays for analytes related to environmental contamination (metal ions and pesticides), and for early diagnosis and monitoring of diseases, using medically important biomarkers.

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“…On amplification of the target gene by PCR, high sensitivity was observed in comparison with agarose gel electrophoresis. Detection limit of the probe was 21.78 ng/µL (Majdinasab et al, 2012). AuNPs assembled via 2-aminoethanethiol on the surface of gold chip were immobilized with gold-binding polypeptides (GBP)protein A-human IgG conjugated to anti-Salmonella Ab.…”

Section: Sensing the Nucleic Acids Of Pathogensmentioning

confidence: 99%