Detection of<i>Listeria monocytogenes</i>from Fresh and Marine Water Fish using Real Time PCR, PCR and Standard ISO Methods

Salamoura, C.; Papadopoulou, Chrissanthy; Filioussis, George; Dontorou, C.; Zakas, G.; Levidiotou, S.; Malamas, Michalis

doi:10.1002/9783527611904.ch50

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(1 citation statement)

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“…This organism could pose a serious public health threat drug for managing listeriosis in pregnant women (Hof, 2004), it is desirable that this favourable situation (lack of resistance observed in this study) is preserved through the establishment of antimicrobial stewardship programmes emphasizing on personal hygiene of frozen fish sellers/buyers. The 100% susceptibility recorded in this work is consistent with the findings of Salamoura et al (2008)…”

Section: Discussionsupporting

confidence: 91%

Epidemiology and antibiogram of listeria species from retail raw frozen atlantic mackerel fish (Scomber scombrus) in Nigeria

Okorie-Kanu¹,

Anyanwu²,

Okosi³

et al. 2023

Nig. Vet. J.

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This study was undertaken to determine the epidemiology and antibiogram of Listeria species isolated from raw frozen Atlantic Mackerel (Scomber Scombrus) fish sold in Enugu State Southeastern Nigeria. Twentyfive grams each of raw fish gut and skin were collected from 800 raw fish sold in 8 major markets randomly selected from the study area. Isolation of Listeria was done using half and full fraser broths, and polymyxin acriflavine lithium chloride ceftazidime aesculin mannitol (PALCAM) agar. Confirmation of the genus Listeria was done by a polymerase chain reaction. Identification of species was done using an analytic profile index (API) kit specific for Listeria. The antibiotic resistance of the isolates was determined using disc diffusion method. Out of the 800 fish samples, 38 (4.75%) were positive for Listeria species. Enugu North (6.50%) had significantly (P<0.05) higher prevalence than Enugu East (3.0%). Five Listeria species were identified. Listeria innocua (71.1%) predominated, followed by Listeria welshimeri (10.5%), Listeria ivanovii (7.9%), Listeria grayi (5.3%) and Listeria monocytogenes (5.3%). All the isolates were susceptible to the tested antibacterial agents. The occurrence of Listeria species in raw frozen Atlantic mackerel fish, poses a threat, when consumed raw or lightly cooked, to people especially the immunocomprormised persons whose population is increasing as a result of improvement in medical technology. Also, the possibility of cross contamination in the kitchen or food service establishments is also a concern. Since all the Listeria species got in this study showed 100% susceptibility to all the antimicrobials tested, therefore, there is no fear of acquiring and transferring antibiotic resistance genes from raw frozen Atlantic mackerel fish to the general human population.

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Section: Discussionsupporting

confidence: 91%