Detection of<i>Listeria monocytogenes</i>in ready-to-eat food by Step One real-time polymerase chain reaction

Pochop, Jaroslav; Kačániová, Miroslava; Hleba, Lukáš; Lopašovský, Ľubomí­r; Bobková, Alica; Zeleňáková, Lucia; Stričík, Michal

doi:10.1080/03601234.2012.634353

Cited by 11 publications

(7 citation statements)

References 26 publications

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“…Thus, these results proved that the real-time PCR can be useful as a rapid diagnostic test for the direct detection of pathogens in food, without the need of enrichment steps. [35,36] The MICs of propolis samples ranged from 8 to 512 µg.mL −1 ( Table 7). The control sample (chloramphenicol) did not have any effect on the growth of bacteria (data not shown).…”

Section: Resultsmentioning

confidence: 99%

In vitroandIn vivoantimicrobial activity of propolis on the microbiota from gastrointestinal tract of chickens

Kačániová

Rovná

Arpášová

et al. 2012

Journal of Environmental Science and Health, Part A

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The aim of this study was to examine the effect of propolis extracts on the microbial colonization of chicken gastrointestinal tract in vivo. The propolis was administered to both feed mixtures in various amounts except of the control group. The addition of 150 mg propolis to 1 kg of feed was included in the first experimental group, the addition of 450 mg.kg(-1) in the second experimental group, the addition of 600 mg.kg(-1) the third experimental group and 800 mg kg(-1) in the fourth one. The highest count of faecal enterococci was found in the third group (8.6 cfu.g(-1)) where 600 mg of propolis to 1 kg was added to the feed mixture. The highest count of lactobacilli was detected in the fourth experimental group (8.83 cfu.g(-1)) where was 800 mg of propolis added to 1 kg of feed mixture and number of Enterobacteriaceae genera count was found in control group (8.73 cfu.g(-1)). With RTQ PCR detected species from the genus Enterococcus were: E. avium, E. casseliflavus, E cecorum, E. faecalis, E. faecium, E. gallinarum, E. hirae and E. malodoratus and from genus Lactobacillus were: Lactobacillus crispatus, L. acidophilus and L. salivarius. With MALDI TOF MS Biotyper from Enterobacteriaceae genera were identified Citrobacter braakii, Raoultella ornithinolytica, Serratia fonticola, Escherichia coli and Klebsiella oxytoca. Antimicrobial activities In vitro of six species of bacteria isolated from gastrointestinal tract of chickens were also tested. The best antimicrobial effect of Citrobacter braakii on ethanolic propolis extract in all concentrations were found.

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Section: Resultsmentioning

confidence: 99%

In vitroandIn vivoantimicrobial activity of propolis on the microbiota from gastrointestinal tract of chickens

Kačániová

Rovná

Arpášová

et al. 2012

Journal of Environmental Science and Health, Part A

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“…This tool for evaluating of birch pollen allergens was used before by Ziarovsk a et al [17] Longhi et al [18] used real-time PCR as a rapid, accurate, and automated tool for the detection and quantification of airborne allergenic pollen taxa. Real-time PCR is also a useful tool for detection: gene expression [19] identification and quantification of pathogens or allergens, [20][21][22] and authentication of food. [23,24] The aim of the study was to determinate and compare relative quantity of hazelnut allergens Corylus avellana, L. CorA and Corylus avellana, L. pollen profiling in pollen samples from different Ukraine areas by real-time PCR.…”

Section: Introductionmentioning

confidence: 99%

Variability ofCorylus Avellana, L. CorA and profilin pollen allergens expression

Ražná

Bežo

Nikolaieva

et al. 2014

Journal of Environmental Science and Health, Part B

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Corylus avellana is the source of inhalant allergies induced by hazel pollen as well as food allergies induced after ingestion of hazelnuts. In this study, real-time PCR approach was used to analyse expression of hazel pollen allergens on the molecular level. Relative quantity of hazelnut allergens Corylus avellana, L. CorA and Corylus avellana, L. pollen profiling in samples from different Ukraine areas were determining and comparing. Differences among the levels of both analysed allergen transcripts were found for hazel CorA and profillin. In both cases, the expression within the urbanized growth conditions was higher when compared to the sample from village area. The average expression for CorA was 0.84 times higher than for profilin and the results are very variable depending on the place of growth. Expression levels here were within the range of 2.957 up to the 52.936. Profilin expression was the highest in the sample from the polluted place of growth-cement plant area with the value of 52 times higher when compared to the sample from the village area. In this study, comparison of expression levels of hazel CorA and profiling pollen allergens was performed for the first time. Real-time PCR assay developed in this study proved the sensitivity for detection of the changes of the hazel pollen allergens expression levels and could benefit labs by fast and reproducible detection method of these allergens.

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“…Longhi et al [24] used real-time PCR as a rapid, accurate, and automated tool for the detection and quantification of airborne allergenic pollen taxa. Real time PCR is a useful tool for detection: gene expression [25] identification and quatification of pathogens, [26][27][28][29] authentication of food. [30] The aim of the study was to determinate relative quantity of birch allergen Betv1 in pollen samples from two Ukraine regions by RT-qPCR.…”

Section: Introductionmentioning

confidence: 99%

Changes in expression of BetV1 allergen of silver birch pollen in urbanized area of Ukraine

Žiarovská

Labajová

Ražná

et al. 2013

Journal of Environmental Science and Health, Part A

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The aim of the study was to determinate the level of expression of silver birch allergen Betv1 in pollen samples from different Ukraine areas by RT-qPCR SYBR Green assay. Protocol for quantifying the expression of Betv1 allergen was developed when testing of three housekeeping genes-cyclophylin, alpha-tubulin and transcription factor CBF1. Samples from urbanized area was analysed by real-time PCR when a sample from forest growth conditions was used as a calibrator. Real-time PCR based quantifying of Betv1 provides a useful method for rapid and sensitive analyses of this silver birch allergen. Our results show higher expression levels in samples from central parts of urbanized area as housing estates when compared to the samples from borders of the urbanized area.

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Detection ofListeria monocytogenesin ready-to-eat food by Step One real-time polymerase chain reaction

Cited by 11 publications

References 26 publications

In vitroandIn vivoantimicrobial activity of propolis on the microbiota from gastrointestinal tract of chickens

In vitroandIn vivoantimicrobial activity of propolis on the microbiota from gastrointestinal tract of chickens

Variability ofCorylus Avellana, L. CorA and profilin pollen allergens expression

Changes in expression of BetV1 allergen of silver birch pollen in urbanized area of Ukraine

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