Detection of<i>Rhynchophorus palmarum</i>(Coleoptera: Curculionidae) and Identification of Associated Nematodes in South Texas

Esparza-Diaz, Gabriela; Olguin, Alma; Carta, Lynn K.; Skantar, Andrea M.; Villanueva, Raul T.

doi:10.1653/024.096.0433

Cited by 12 publications

(8 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A number of Panagrellus species have been found in phoretic association with beetles such as P. leperisini, P. dorsibidentatus, and P. dubius (in frass) (Stock and Nadler, 2006), and many reports of association with fruit flies in nature and the laboratory (Goodey, 1963). Thus far, to the best of our knowledge, panagrolaimid nematodes were never reported in association with this weevil pest (Mazza et al, 2014), nor were they reported in the related weevil species Rhynchophorus palmarum that contained nematodes from Rhabditidae, Diplogastridae (Rhabditida), and Parasitaphelenchidae (Aphelenchida) (Esparza-D ıaz et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Occurrence of Panagrellus (Rhabditida: Panagrolaimidae) Nematodes in a Morphologically Aberrant Adult Specimen of Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae)

Camerota

Mazza

Carta

et al. 2016

Journal of Nematology

Self Cite

View full text Add to dashboard Cite

An aberrant specimen of Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae) also known as red palm weevil (RPW), the most economically important insect pest of palms in the world, was found among a batch of conspecifics reared for research purposes. A morphological analysis of this weevil revealed the presence of nematodes associated with a structured cuticle defect of the thorax. These nematodes were not able to be cultured, but were characterized by molecular analysis using 28S and 18S ribosomal DNA and shown to belong to the family Panagrolaimidae (Rhabditida), within a clade of Panagrellus. While most nematodes in the insect were juveniles, a single male adult was partially characterized by light microscopy. Morphometrics showed similarities to a species described from Germany. Excluding the entomopathogenic nematodes (EPN), only five other genera of entomophilic or saprophytic rhabditid nematodes are associated with this weevil. This is the first report of panagrolaimid nematodes associated with this invasive pest. Possible mechanisms of nematode-insect association are discussed.Key words: insect thorax defect, invasive species, nematode phoresy, physiological ecology, saprophagous nematode, sour paste nematode.Rhynchophorus ferrugineus, the RPW, is currently considered as the most damaging pest of palm species in the world (Giblin-Davis et al., 2013). A recent review highlighted the paucity of data concerning the natural enemies and the organisms associated with the RPW (Mazza et al., 2014). Although new knowledge has been acquired in recent years, much remains to be done (Mazza et al., 2014). Excluding the EPN belonging to Steinernema and Heterorhabditis genera, few other insectassociated nematodes with entomophilic, saprobiotic, phoretic, commensal, or parasitic associations (Sudhaus, 2008) are known to be associated with this weevil. Those genera already identified include Acrostichus Rahm, 1928; Bursaphelenchus Fuchs, 1937; Caenorhabditis (Osche, 1952) Dougherty, 1953 Diplogasteritus Paramonov, 1952; Mononchoides Rahm, 1928, and Teratorhabditis (Osche, 1952) Dougherty, 1953 (reviewed in Troccoli et al., 2015. Further studies are necessary to identify weevil nematodes, clarify their biology and type of association with RPW, and consider their possible effects as biocontrol agents (Mazza et al., 2014). With this aim, we report the first finding of nematodes belonging to Panagrolaimidae (Nematoda: Rhabditida) associated with the RPW and briefly discuss the possible modes of association. MATERIALS AND METHODSOrigin and rearing of the RPW: Newly emerged RPW adults with a balanced sex ratio (n = 384) were provided by the UTAGRI-ENEA C.R. Casaccia, Laboratorio Gestione Sostenibile degli Agroecosistemi, Rome, Italy in 2013 to 2014. Larvae were individually reared, in a climatic room (298C, about 100% relative humidity with a photoperiod of 12:12), in plastic containers with perforated lids, fed with apple slices, and incubated until adult emergence. Final larval instars made cocoons using coc...

show abstract

Section: Resultsmentioning

confidence: 99%

Occurrence of Panagrellus (Rhabditida: Panagrolaimidae) Nematodes in a Morphologically Aberrant Adult Specimen of Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae)

Camerota

Mazza

Carta

et al. 2016

Journal of Nematology

Self Cite

View full text Add to dashboard Cite

show abstract

“…The observed insect behavior suggested the convenience of volatile chemical analysis. In several weevil species, aggregation pheromones are molecules containing ten carbons or less [ 7 , 8 , 9 ]. Because of this, solid-phase microextraction (SPME) polydimethylsiloxane (PDMS) and a carboxen/divinylbenzene in PDMS fibers were chosen to capture volatiles in the 40–275 g/mol range.…”

Section: Discussionmentioning

confidence: 99%

“…In Mexico, several curculios such as Scyphophorus acupunctatus , Rhynchophorus palmarum , Anthonomus eugenii , and Anthonomus grandis are considered principal crop pests, and they have been extensively studied; these insects attack agave, coconut palm, chili pepper, and cotton plants, respectively [ 7 , 8 , 9 , 10 ]. Weevil pests also attack soursop ( Annona muricata L.), another important Mexican agronomic product.…”

Section: Introductionmentioning

confidence: 99%

α-Terpineol: An Aggregation Pheromone in Optatus palmaris (Coleoptera: Curculionidae) (Pascoe, 1889) Enhanced by Its Host-Plant Volatiles

et al. 2021

View full text Add to dashboard Cite

The Annonaceae fruits weevil (Optatus palmaris) causes high losses to the soursop production in Mexico. Damage occurs when larvae and adults feed on the fruits; however, there is limited research about control strategies against this pest. However, pheromones provide a high potential management scheme for this curculio. Thus, this research characterized the behavior and volatile production of O. palmaris in response to their feeding habits. Olfactometry assays established preference by weevils to volatiles produced by feeding males and soursop. The behavior observed suggests the presence of an aggregation pheromone and a kairomone. Subsequently, insect volatiles sampled by solid-phase microextraction and dynamic headspace detected a unique compound on feeding males increased especially when feeding. Feeding-starvation experiments showed an averaged fifteen-fold increase in the concentration of a monoterpenoid on males feeding on soursop, and a decrease of the release of this compound males stop feeding. GC-MS analysis of volatiles identified this compound as α-terpineol. Further olfactometry assays using α-terpineol and soursop, demonstrated that this combination is double attractive to Annonaceae weevils than only soursop volatiles. The results showed a complementation effect between α-terpineol and soursop volatiles. Thus, α-terpineol is the aggregation pheromone of O. palmaris, and its concentration is enhanced by host-plant volatiles.

show abstract

“…Individual insects can simultaneously harbor more than one nematode genus (Grucmanová, Holuša, Čermák, & Nermut, ; Kanzaki et al, ; Shimizu et al, ); R. palmarum and R. cruentatus are reported to host several nematode associates, including Teratorhabditis and Mononchoides spp. (Esparza‐Diaz et al, ; Kanzaki et al, , , ; Mazza et al, ; Sudhaus et al, ). This finding confirmed the possibility of vertical transmission between Teratorhabditis and Mononchoides nematode genera and the insect genus Rhynchophorus (Kanzaki et al, ; Troccoli et al, ), suggesting that the ecological niche plays an important role in shaping the nematode community of Rhynchophorus spp.…”

Section: Discussionmentioning

confidence: 99%

“…Associations between Rhynchophorus species and nematodes are well documented for R. palmarum (L.) and R. cruentatus (F.), but very little is known about R. ferrugineus with regard to its fitness, evolution, coadaptation, speciation, defense, chemical communication, or pest management (Camerota et al, 2016;Esparza-Diaz, Olguin, Carta, Skantar, & Villanueva, 2013;Giblin-Davis et al, 2013;Kanzaki & Giblin-Davis, 2018). Nematodes are microscopic worms that are adapted to living in a variety of environments, and many can be associated with other organisms that provide them with shelter or transportation, for example, entomophilic, saprobiotic, phoretic, commensal, and parasitic nematodes (Kanzaki, 2017).…”

mentioning

confidence: 99%

Molecular profiling of nematode associates with Rhynchophorus ferrugineus in southern Italy

Luca

Fanelli

Oreste

et al. 2019

Ecology and Evolution

View full text Add to dashboard Cite

A survey of nematodes associated with the red palm weevil Rhynchophorus ferrugineus was conducted in southern Italy in 2015 and 2016 in order to create a species inventory and obtain data about nematode biodiversity. A total of 70 insect samples (pupae and adults) were collected from infested Phoenix canariensis, Phoenix dactylifera, and Chamaerops humilis palms in three Italian Regions: sampling took place at 11 locations in Apulia, 1 in Basilicata, and 1 in Sardinia regions. Individual insects were dissected to determine nematode presence, and different nematode species were also recovered from red palm weevil cocoons collected at the sites in Apulia. Individual nematodes were molecularly identified by sequencing the ITS, D2‐D3 expansion domains of the 28SrRNA gene and the mitochondrial COI and inferring the phylogenetic relationships. The insect‐associated nematofauna identified belonged to the families Rhabditidae, Cephalobidae, and Diplogastridae. Just two nematode species, Teratorhabditis synpapillata and Mononchoides macrospiculum, were always found in association with adult insects and cocoons taken from all sampling sites. This paper reports on the biodiversity of the nematodes associated with R. ferrugineus and on current knowledge of the specific habitat of specialized and divergent entomophilic nematodes.

show abstract

Detection ofRhynchophorus palmarum(Coleoptera: Curculionidae) and Identification of Associated Nematodes in South Texas

Cited by 12 publications

References 17 publications

Occurrence of Panagrellus (Rhabditida: Panagrolaimidae) Nematodes in a Morphologically Aberrant Adult Specimen of Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae)

Occurrence of Panagrellus (Rhabditida: Panagrolaimidae) Nematodes in a Morphologically Aberrant Adult Specimen of Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae)

α-Terpineol: An Aggregation Pheromone in Optatus palmaris (Coleoptera: Curculionidae) (Pascoe, 1889) Enhanced by Its Host-Plant Volatiles

Molecular profiling of nematode associates with Rhynchophorus ferrugineus in southern Italy

Contact Info

Product

Resources

About