Detection ofSalmonellain Finishing Pigs on Farm and at Slaughter in Piedmont, Italy

Abstract

Salmonella is one of the most common causes of human gastroenteritis often associated with pork consumption. The aims of this cross-sectional study were to collect preliminary data on the presence of Salmonella enterica in pigs in Piedmont (Italy), through sampling on farm and at slaughter and to gather pilot data on serotypes and phagetypes present in the sampled area and distribution of anti-microbial resistance among isolated strains. Salmonella was detected through culture and identified with Salmonella spp. and Salmonella Typhimurium PCR; positive samples were serotyped, phagetyped and tested for antibiotic susceptibility. Positive samples (from 9% of faeces up to 29% of tonsils) were found in 64% of the herds. Salmonella spp. was retrieved also from scalding water. Most of the isolates were Salmonella Derby, Salmonella Typhimurium and Salmonella 4,5,12:i:-. The results of Salmonella Typhimurium specific PCR suggested that Salmonella 4,5,12:i:- might be unrecognized by serotyping. Anti-microbial resistance was recorded in 75-100% of the isolates. Phagetyping allowed the identification of DT104B and DT46A strains. These results set the bases for further research studies that would aim to estimate the real herd prevalence in Piedmont and the diffusion of serotypes and anti-microbial resistant strains within the same region.

Prevalence and antimicrobial resistance of Salmonella spp. and Escherichia coli isolated from pigs at slaughterhouses in Korea

“…In Germany, Salmonella was isolated from 3.7% of the feces at seven slaughterhouses in 1996 (Käsbohrer et al, 2000). In Italy, 9.33% of the feces were positive when examined using polymerase chain reaction (Lomonaco et al, 2009). In this study, the Salmonella prevalence was relatively low level (2.5%), and is markedly lower than that reported (21%) in another study conducted in Korea (Suh et al, 2005).…”

“…The entry of Salmonella carrier pigs into slaughterhouses is a major source of meat contamination and of introduction of Salmonella into the food chain (McDowell et al, 2007;Käsbohrer et al, 2000;Berends et al, 1997;Padungtod et al, 2008;Padungtod et al, 2006). For this reason, several studies have investigated the Salmonella prevalence from the feces of pigs in slaughterhouses and farms (Käsbohrer et al, 2000;Lomonaco et al, 2009). In Germany, Salmonella was isolated from 3.7% of the feces at seven slaughterhouses in 1996 (Käsbohrer et al, 2000).…”

“…In Europe and America, S. rissen is an uncommom serotype (McDowell et al, 2007;Käsbohrer et al, 2000;Mainar-Jaime et al, 2008;Lomonaco et al, 2009). On the other hand, similar, to the present study, S. rissen was the most common serotype in a report from Thailand (Padungtod et al, 2006).…”

“…Pigs are a major reservoir of bacterial zoonotic pathogens such as Salmonella (McDowell et al, 2007;Käsbohrer et al, 2000;Mainar-Jaime et al, 2008;Lomonaco et al, 2009), Campylobacter (Padungtod et al, 2008;Denis et al, 2009) and Escherichia coli (Teshager et al, 2000). Except for self-consumption and scientific *Corresponding author.…”

Sub-Iliac Lymph Nodes at Slaughter Lack Ability to PredictSalmonella entericaPrevalence for Swine Farms

“…Salmonella Typhimurium is the major cause of human salmonellosis (Olsen et al, 1997;Fullerton et al, 2007). Salmonella Derby has been frequently associated with pork ( Jayarao et al, 1990;Davies et al, 1997;Valdezate et al, 2005;Lomonaco et al, 2009), but not human infections.…”