Detection of
 Toxoplasma gondii
 oocysts in soil and risk mapping in an island environment in the Northeast of Brazil

Oliveira, Pollyanne Raysa Fernandes de; Melo, Renata Pimentel Bandeira de; Oliveira, Umberto Diego Rodrigues de; Magalhães, Fernando Jorge Rodrigues; Ferreira, Rivaldo José; Ribeiro-Andrade, Müller; Mota, Rinaldo Aparecido

doi:10.1111/tbed.14705

Cited by 7 publications

(3 citation statements)

References 99 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Considering the high incidence of Toxocara spp. and T. gondii observed in other studies [42][43][44],…”

Section: Discussionmentioning

confidence: 56%

Soil-Transmitted Parasites and Non-Pathogenic Nematodes in Different Regions of Porto Alegre City, Brazil: A Comparison between Winter and Summer

Ziliotto,

Ellwanger,

Chies

2023

Parasitologia

View full text Add to dashboard Cite

We assessed the prevalence of soil-associated parasites and non-pathogenic nematodes in eight public areas of Porto Alegre (Rio Grande do Sul state, southern Brazil), the most populous city in Rio Grande do Sul. Soil samplings were carried out during the winter of 2022 and summer of 2023: A total of 80 samples were collected in winter and 80 in summer (ten samples from each sampling site per season), totaling 160 soil samples. The frequency of microscopic non-pathogenic nematode larvae was significantly higher (p = 0.048) in winter (93.75%) than in summer (82.50%). Considering the pooled data from winter and summer (n = 160) for human pathogenic parasites, the following frequencies were observed (using microscopy analysis): hookworm (filariform) larvae (1.25%), hookworm (rhabditiform) larvae (11.25%), Strongyloides spp. (filariform) larvae (0.63%), Strongyloides spp. (rhabditiform) larvae (2.5%), hookworm eggs (10.63%), Ascaris spp. eggs (10.00%), and Trichuris spp. eggs (1.25%). Hookworm (rhabditiform) larvae were the most frequent parasitic structures (15.00%) in winter, and A. lumbricoides eggs were the most frequent parasitic structures (8.75%) in summer. No statistically significant difference was observed in the frequency of pathogenic parasites between the seasons (p > 0.05). Toxoplasma gondii DNA was assessed, but all soil samples tested negative in molecular analysis. Our results indicate that soil from many regions of Porto Alegre shows a high prevalence of soil-transmitted helminths, indicating the need for improvements in social conditions and environmental sanitation in the city. Our study also suggests that climate change may affect soil biodiversity, potentially harming non-pathogenic nematodes and favoring human pathogenic parasites.

show abstract

“…Considering the high incidence of Toxocara spp. and T. gondii observed in other studies [42][43][44],…”

Section: Discussionmentioning

confidence: 56%

Soil-Transmitted Parasites and Non-Pathogenic Nematodes in Different Regions of Porto Alegre City, Brazil: A Comparison between Winter and Summer

Ziliotto,

Ellwanger,

Chies

2023

Parasitologia

View full text Add to dashboard Cite

show abstract

“…Felid species, including domestic cats and their relatives, are the definitive hosts and the only hosts that can shed oocysts into the environment [5]. Environmental contamination is an important health issue, therefore monitoring of the occurrence of this parasite is crucial for the One Health approach [6,7]. Rodents pose a particular threat to public health because they are intermediate hosts of T. gondii and are also the reservoirs of infection for other animals [8].…”

Section: Introductionmentioning

confidence: 99%

Identification of Toxoplasma gondii in wild rodents in Poland by molecular and serological techniques

Nowicka,

Antolova,

Lass

et al. 2024

Ann Agric Environ Med.

View full text Add to dashboard Cite

Rodents are recognized as reservoirs for Toxoplasma gondii, playing a crucial role in maintaining the parasite's presence in the environment. Biomonitoring was conducted to assess the role of sylvatic rodents in maintaining T. gondii, and to analyse the prevalence and seroprevalence of the parasite in seven wild rodent species. Rodents were collected in an open grassland study site located in northeastern Poland, and dissected. Brain, spleen, blood and serum samples were collected. Molecular (PCR assay, nested-PCR assay) and serological (ELISA and agglutination tests) methods were applied to indicate the best approach for application in the biomonitoring of T. gondii in small mammals. Samples were screened from 68 individuals using PCR assays but no T. gondii DNA were found. The agglutination test showed no signal. Antibodies against T. gondii were found in 5 sera samples out of 56 analysed (seroprevalence = 8. 9% [4.4-16.8]). The results confirmed that rodents participate in the life cycle of T. gondii as reservoirs of this parasite in the sylvatic environment. However, for effective bio-monitoring of T. gondii in small mammals, the results suggest a preference for utilizing ELISA tests to detect T. gondii antigens, as opposed to relying solely on molecular methods.

show abstract

“…Felid species, including domestic cats and their relatives, are the definitive hosts and the only hosts that can shed oocysts into the environment [5] . Environmental contamination is an important health issue, therefore detecting the occurrence of this parasite is crucial for the One Health approach [6,7]. Rodents pose a particular threat to public health because they are considered to be reservoirs (sources of T. gondii infection for other organisms) and are intermediate hosts for T. gondii [8].…”

Section: Introductionmentioning

confidence: 99%

Detection of Toxoplasma gondii in Sylvatic Rodents in Poland Using Molecular and Serological Methods

Nowicka¹,

Antolová²,

Lass³

et al. 2023

Preprint

View full text Add to dashboard Cite

Rodents are known to be reservoirs of Toxoplasma gondii and keep the parasite circulation in the environment. We conducted biomonitoring to assess the role of sylvatic rodents in maintaining T. gondii and to analyse the prevalence and seroprevalence of the parasite in seven wild rodent species. Rodents were collected in our study sites (woodland and open grasslands) located in northeastern Poland and dissected. We collected brain, spleen, blood and serum samples. We applied both molecular (PCR assay, nested-PCR assay) and serological (ELISA and agglutination tests) methods to indicate the best approach for application in the biomonitoring of T. gondii in small mammals. We screened samples from 95 individuals sing PCR assays and found no T. gondii DNA. The agglutination test showed no signal. We found antibodies against T. gondii in 6 sera samples out of 74 analysed (seroprevalence = 8.11% [4.0-17.1]). Our results confirm that rodents participate in the life cycle of T. gondii as reservoirs of this parasite in the sylvatic environment. However, biomonitoring should be performed with the ELISA tests to search for T. gondii antigens, rather than a molecular approach only.

show abstract

Detection of Toxoplasma gondii oocysts in soil and risk mapping in an island environment in the Northeast of Brazil

Cited by 7 publications

References 99 publications

Soil-Transmitted Parasites and Non-Pathogenic Nematodes in Different Regions of Porto Alegre City, Brazil: A Comparison between Winter and Summer

Soil-Transmitted Parasites and Non-Pathogenic Nematodes in Different Regions of Porto Alegre City, Brazil: A Comparison between Winter and Summer

Identification of <i>Toxoplasma gondii</i> in wild rodents in Poland by molecular and serological techniques

Detection of <em>Toxoplasma gondii</em> in Sylvatic Rodents in Poland Using Molecular and Serological Methods

Contact Info

Product

Resources

About