Detection of
            <i>Trichomonas vaginalis</i>
            on Modified Columbia Agar in the Routine Laboratory

Stary, Angelika; Kuchinka-Koch, Angelika; Teodorowicz, L

doi:10.1128/jcm.40.9.3277-3280.2002

Cited by 29 publications

(20 citation statements)

References 26 publications

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“…The second vaginal swab was placed in phosphate buffer saline solution and inoculated in Modified Columbia Agar (Columbia agar and Malt extract Oxoid ltd Basingstoke HANTS England, Dextrose Oxoid ltd Basingstoke Hampshire England, Streptomycin and Penicillin CSPC Zhongnuo Pharmaceutical (Shijiazhuang) co. ltd China, Chloramphenicol KARNATAKA antibiotics and pharmaceuticals limited India, Nystatin (Mycostatin) Amman pharmaceutical industries co. Jordan) in petri dishes. Culture and identification of T. vaginalis were done following the procedures as described by [11].…”

Section: Data Collectionmentioning

confidence: 99%

Trichomonas vaginalis Infection among Pregnant Women in Jimma University Specialized Hospital, Southwest Ethiopia

Eshete

Mekonnen

Zeynudin

2013

ISRN Infectious Diseases

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Background. Trichomonas vaginalis is a sexually transmitted parasitic protozoan known to be responsible for an estimated 180 million new infections per year, making it the most prevalent nonviral sexually transmitted pathogen worldwide. Method. A crosssectional study design was conducted on vaginal swabs by wet mount and Modified Columbia Agar culture technique in Jimma University Specialized Hospital (JUSH), ANC clinic, Jimma, Ethiopia. The study was done to assess the magnitude and associated risk factors of T. vaginalis infection from December to May, 2011/2012. Result. A total of 361 pregnant women were involved in this study. From these, 18 (4.98%) of the pregnant women were positive for T. vaginalis infection by Modified Columbian Agar culture technique. Education status (AOR = 0.186, 95% CI: 0.059-0.585, < 0.05), patients with dysuria (AOR = 0.180; 95% CI: 0.046-0.704, < 0.05) and dyspareunia (AOR = 0.152; 95% CI: 0.035-0.667, < 0.05) were significantly associated with T. vaginalis infection. Conclusion. The prevalence of T. vaginalis infection at 4.89% is relatively high among young reproductive aged women. Because this infection increases the risk of HIV transmission and is associated with adverse pregnancy outcomes, there is a need for increased provision of health information concerning T. vaginalis to the community, educating women, screening, and treatment of T. vaginalis infection in Ethiopia.

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Section: Data Collectionmentioning

confidence: 99%

Trichomonas vaginalis Infection among Pregnant Women in Jimma University Specialized Hospital, Southwest Ethiopia

Eshete

Mekonnen

Zeynudin

2013

ISRN Infectious Diseases

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“…In the case of direct microscopic examinations, the detection of T. vaginalis depends on the microscopist's expertise and on the swift transport and processing of the sample, in order to avoid the loss of motility of the parasite [4]. Consequently, the liquid culture medium for T. vaginalis is considered the most accurate method (gold-standard) for the diagnosis of trichomoniasis [6,[10][11][12][13].The objectives of this work were to survey the prevalence of infection in asymptomatic and symptomatic pregnant women and to assess the utility of different microscopic methodologies for the diagnosis of trichomoniasis in pregnant women and to compare them with the method using the liquid culture medium.A total of 597 vaginal exudates from pregnant women were …”

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confidence: 99%

Prevalence and Comparison of Diagnostic Methods for Trichomonas vaginalis Infection in Pregnant Women in Argentina

Perazzi

Menghi

Coppolillo³

et al. 2010

Korean J Parasitol

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Hormone changes produced during pregnancy predispose a higher incidence of infections of the lower genital tract. This leads to maternal and perinatological complications. The diagnosis of Trichomonas vaginalis infection during pregnancy is of great importance as such infections are related to premature rupture of membranes, preterm delivery, and low birth weight [1,2].Trichomoniasis is a sexually-transmitted disease associated with urethritis, vaginitis, cervicitis, pelvic inflammatory disease, and tubal infertility [3][4][5], and it is also linked to cervical cancer [6]. Up to 10 to 50% of infected women are asymptomatic and 50% of these women will develop clinical symptoms during the subsequent 6 months [7]. Moreover, reinfection may occur by sexual contact with the man who acts as an asymptomatic carrier [8].The laboratory diagnosis by microscopic examination has low sensitivity (35-80%) mainly in asymptomatic patients due to the low number of organisms in the sample. This is why it is necessary to implement more sensitive methodologies [9]. In the case of direct microscopic examinations, the detection of T. vaginalis depends on the microscopist's expertise and on the swift transport and processing of the sample, in order to avoid the loss of motility of the parasite [4]. Consequently, the liquid culture medium for T. vaginalis is considered the most accurate method (gold-standard) for the diagnosis of trichomoniasis [6,[10][11][12][13].The objectives of this work were to survey the prevalence of infection in asymptomatic and symptomatic pregnant women and to assess the utility of different microscopic methodologies for the diagnosis of trichomoniasis in pregnant women and to compare them with the method using the liquid culture medium.A total of 597 vaginal exudates from pregnant women were Prevalence and Comparison of Diagnostic Methods for Trichomonas vaginalis Infection in Pregnant Women in ArgentinaKorean J Parasitol. Vol. 48, No. 1: 61-65, March 2010 DOI: 10.3347/kjp.2010 61 Abstract: The objectives of this study were to conduct a prevalence survey of trichomoniasis in pregnant women and to evaluate the utility of different methods for its diagnosis. A total of 597 vaginal exudates from pregnant women who were examined at the Hospital de Clinicas in Buenos Aires, Argentina from 1 August 2005 to 31 January 2007, were prospectively and consecutively evaluated. The investigation of Trichomonas vaginalis was made by different microscopic examinations, and culture on liquid medium. The sensitivity and specificity of the microscopic examinations were assessed considering culture on liquid medium as the "gold standard". The prevalence of T. vaginalis obtained by culture on liquid medium was 4.0% (24/597). The prevalence of T. vaginalis obtained by direct wet smear, prolonged May-Grunwald Giemsa staining, and sodium acetate-formalin (SAF)/methylene blue staining-fixing technique was 1.8%, 2.3% and 2.5%, respectively. The sensitivity of the direct wet smear was 45.8%, that of the prolonged May-Grunwald Giemsa s...

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“…The prevalence of T. vaginalis in each age group depends on several factors, including the technique used to assess this prevalence [17][18][19][20]. For example, research using culture, wet mount, and Pap smear found a prevalence of 2.6% in women attending primary health clinics in Uberlândia, MG, in the southeast region of Brazil [18].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Trichomonas vaginalis in women of reproductive age at a family health clinic

Glehn

Sá

Silva

et al. 2017

J Infect Dev Ctries

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Introduction: Trichomonas vaginalis is considered the most prevalent curable sexually transmitted infection, and its occurrence exceeds that of gonococcal and chlamydia infections. This parasite has been identified as responsible for the increased risk of transmission of HIV and has also been associated with prostate and cervical cancer. Many carriers of T. vaginalis are asymptomatic and, when experiencing a health problem, they most often have nonspecific symptoms. The aim of this research was to estimate the presence of T. vaginalis and the associated factors in women of childbearing age at a primary health care clinic in the Federal District of Brazil. Methodology: A cross-sectional study was conducted with consecutive sampling of an outpatient population of women of childbearing age (excluding minors and pregnant women). The women answered a questionnaire and were examined. After vaginal pH measurement and whiff testing, a vaginal secretion sample was obtained for inoculation in TYM, a specific T. vaginalis culture medium. Results: The presence of T. vaginalis was identified in 16% of the sample. Fewer lifetime sexual partners and consistent condom use were identified as factors of protection against the infection. Complaints of dyspareunia were proportionally higher among women with positive cultures for T. vaginalis. Conclusions: The prevalence of T. vaginalis infection was high in the sample studied. The infection was positively associated with the number of lifetime sexual partners, and consistent condom use was a protective factor. Vaginal complaints were more common among women with T. vaginalis, but only dyspareunia had significant association.

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Detection of Trichomonas vaginalis on Modified Columbia Agar in the Routine Laboratory

Cited by 29 publications

References 26 publications

Trichomonas vaginalis Infection among Pregnant Women in Jimma University Specialized Hospital, Southwest Ethiopia

Trichomonas vaginalis Infection among Pregnant Women in Jimma University Specialized Hospital, Southwest Ethiopia

Prevalence and Comparison of Diagnostic Methods for Trichomonas vaginalis Infection in Pregnant Women in Argentina

Prevalence of Trichomonas vaginalis in women of reproductive age at a family health clinic

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