Detection of IgG and IgM in Sera from Canines with Blastomycosis using Eight Blastomyces dermatitidis Yeast Phase Lysate Antigens

Sestero, Christine M.; Scalarone, Gene M.

doi:10.1007/s11046-006-0028-7

Cited by 20 publications

(15 citation statements)

References 18 publications

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“…Sporothrix schenckii (Ssl7 - St. John's Institute of Dermatology, London, United Kingdom) yeasts were grown in Brain Heart Infusion (BHI) broth for 3 days at 37°C [31]. Blastomyces dermatitidis (NCPF 4076-National Collection of Pathogenic Fungi, Colindale, London, United Kingdom) yeasts were grown in BHI at 37°C for five days [32]. A. fumigatus (strain 3111 – Laboratório de Micologia, Instituto de Pesquisa Clínica Evandro Chagas, FIOCRUZ, Brazil) was grown in Sabouraud Broth at 37°C for 96 h (cellular extract) or 3 weeks (culture filtrate).…”

Section: Methodsmentioning

confidence: 99%

Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum

et al. 2008

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Histoplasmosis, due to the intracellular fungus Histoplasma capsulatum, can be diagnosed by demonstrating the presence of antibodies specific to the immunodominant M antigen. However, the role of this protein in the pathogenesis of histoplasmosis has not been elucidated. We sought to structurally and immunologically characterize the protein, determine yeast cell surface expression, and confirm catalase activity. A 3D-rendering of the M antigen by homology modeling revealed that the structures and domains closely resemble characterized fungal catalases. We generated monoclonal antibodies (mAbs) to the protein and determined that the M antigen is present on the yeast cell surface and in cell wall/cell membrane preparations. Similarly, we found that the majority of catalase activity was in extracts containing fungal surface antigens and that the M antigen is not significantly secreted by live yeast cells. The mAbs also identified unique epitopes on the M antigen. The localization of the M antigen to the cell surface of H. capsulatum yeast and the characterization of the protein's major epitopes have important implications since it demonstrates that although the protein may participate in protecting the fungus against oxidative stress it is also accessible to host immune cells and antibody.

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Section: Methodsmentioning

confidence: 99%

Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum

et al. 2008

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“…Lysates 48938, B5896 and T‐58 provided high specificity but low sensitivity (as a result of many false negatives). Other studies of lysates 48938 and B5896 have shown these lysates provide good detection levels for antibody to blastomycosis but varying results for T‐58 . On the other hand lysate ER‐598 provides high sensitivity but low specificity.…”

Section: Discussionmentioning

confidence: 99%

Development of a slide agglutination assay for detection of blastomycosis

Hatch

Scalarone

2013

Microbiology and Immunology

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Blastomycosis, caused by the thermally dimorphic fungus Blastomyces dermatitides, which is endemic to eastern regions of the USA, is commonly misdiagnosed as a viral or bacterial infection and therefore treated improperly. Over the years, many immunodiagnostic assays to aid in the diagnosis of blastomycosis have been developed; however, a reliable assay for use in local clinics still remains elusive. Procedures for a slide agglutination assay for detection of antibody in serum from rabbits immunized with B. dermatitidis were evaluated with antigenic preparations from B. dermatitidis adsorbed to polystyrene microparticles. Yeast-phase lysates from five isolates of B. dermatitides: namely ER-593 (Eagle River, WI, USA), ER-598 (Eagle River, WI, USA), 48938 (India), B5896 (Mt. Iron, MN, USA), and T-58 (TN, USA) were evaluated for their sensitivity and specificity. Sensitivities of the lysates ranged from 29% to 83% whereas specificities ranged from 13% to 100%. Lysate ER-593 provided the most promising results with a sensitivity of 82% and specificity of 100%. This study provides suggests that a simple rapid slide agglutination assay for detecting blastomycosis may be used for screening patients with suspected B. dermatitidis infection.

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“…In contrast a one-day period of lysis of H. capsulatum yeast cells was sufficient to produce a reagent that detected both delayed dermal hypersensitivity as well as being useful for the detection of antibodies in the ELISA [19,20]. With regard to our work with B. dermatitidis yeast phase lysate antigens many of our comparative studies have been done with reagents following growth, harvesting and allowing the cells to lyse for a period of 7 days [10,11,13,15,23,24].…”

Section: Discussionmentioning

confidence: 99%

Comparison of antibody detection with <i>Blastomycesdermatitidis</i> yeast lysate antigens in serum specimens from immunized rabbits and infected dogs

Christenson¹,

Horton²,

Campbell³

et al. 2011

OJI

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This present study was designed to evaluate B. dermatitidis antigens, prepared from two isolates (B5896, 597), when the yeast cells were allowed to lyse in distilled water for one day or seven days. The indirect enzyme-linked immunosorbent assay (ELISA) was used to determine the ability of the lysate reagents to detect antibodies in 30 rabbit and 30 dog serum specimens. Mean absorbance values with B5896 lysate antigen ranged from 1.637 (day 1) to 1.461 (day 7) and absorbance values with 597 antigen ranged from 1.579(day 1) to 1.396 (day 7) with the serum specimens from immunized rabbits. Serum specimens from infected dogs yielded absorbance values ranging from 1.672 (day 1) to 1.763 (day 7) with the B5896 and values ranging from 1.909 (day 1) to 1.224 (day 7) with the 597. Optimal reactivity was obtained with the day 1 lysate using both lysate antigens against the rabbit sera and with the 597 antigen against the dog sera. Slightly greater reactivity was evidenced with the day 7 B5896 antigen when the dog sera was tested. Comparative studies are continuing in order to produce an optimal antigenic preparation for antibody detection in blastomycosis.

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Detection of IgG and IgM in Sera from Canines with Blastomycosis using Eight Blastomyces dermatitidis Yeast Phase Lysate Antigens

Cited by 20 publications

References 18 publications

Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum

Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum

Development of a slide agglutination assay for detection of blastomycosis

Comparison of antibody detection with <i>Blastomycesdermatitidis</i> yeast lysate antigens in serum specimens from immunized rabbits and infected dogs

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Detection of IgG and IgM in Sera from Canines with Blastomycosis using Eight Blastomyces dermatitidis Yeast Phase Lysate Antigens

Cited by 20 publications

References 18 publications

Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum

Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum

Development of a slide agglutination assay for detection of blastomycosis

Comparison of antibody detection with &lt;i&gt;Blastomycesdermatitidis&lt;/i&gt; yeast lysate antigens in serum specimens from immunized rabbits and infected dogs

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Comparison of antibody detection with <i>Blastomycesdermatitidis</i> yeast lysate antigens in serum specimens from immunized rabbits and infected dogs