Detection of IL-6, IL-10, and TNF-α level in human single-blastocyst conditioned medium using ultrasensitive Single Molecule Array platform and its relationship with embryo quality and implantation: a pilot study

Zhong, Huixian; Sun, Qing; Chen, Peilin; Xiong, Feng; Li, Guan-Gui; Wan, Caiyun; Yao, Zhihong; Zeng, Yong

doi:10.1007/s10815-020-01805-7

Cited by 5 publications

(3 citation statements)

References 25 publications

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“…However, they could not detect the concentration of GM-CSF in the ECM, suggesting that the concentration of GM-CSF in the ECM may be in the fg/ml range, and therefore, too low to be measurable with Luminex. Previously, we found that SIMOA, a new ultrasensitive protein detection technology, can be used to determine proteins in SBCM at femtomolar concentrations ( 21 ). In the present study, the detection rate of GM-CSF in SBCMs was identified to be 50% (63/126), and the concentration of GM-CSF in the detected SBCM samples was 5.60 (1.95, 14.74) fg/ml.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, further studies with multicenter larger sample size are warranted to obtain a more accurate prediction model for clinical application. Second, it has been reported that embryo-secreted proteins, such as HCG ( 6 , 29 ), soluble form of HLA-G (sHLA-G) ( 30 , 31 ), and IL-6 ( 19 , 21 ) also could be used to predict embryo quality or pregnancy outcome. The predictive model based on multiple proteins in SBCMs may improve the sensitivity and specificity of the prediction model in the further study.…”

Section: Discussionmentioning

confidence: 99%

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Granulocyte-Macrophage Colony Stimulating Factor in Single Blastocyst Conditioned Medium as a Biomarker for Predicting Implantation Outcome of Embryo

et al. 2021

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BackgroundIt is highly desirable to develop new strategies based on secretomics to more accurately selection of embryos with the highest developmental potential for transfer. Granulocyte-macrophage colony-stimulating factor (GM-CSF) has been reported to promote embryo development and pregnancy establishment. However, the predictive value of GM-CSF in single blastocyst selection remains unclear. This study is to determine the concentration of GM-CSF in human single-blastocyst conditioned medium (SBCM) and to evaluate its association with embryo quality and pregnancy outcome.MethodsThe patients with ≤38 years of age receiving the first cycle of assisted reproductive therapy were included in this study. The patients who had <4 top-quality embryos formed by the fertilized two pronuclear zygotes on day 3 were excluded. A total of 126 SBCM samples (SBCMs) were included, of which blastocysts from 77 SBCMs were later transferred in subsequent frozen-thawed embryo transfer. The concentrations of GM-CSF were detected by single-molecule array (SIMOA) and analyzed for their possible association with embryo quality and pregnancy outcomes. The top-quality embryo (TQ), positive HCG (HP), clinical pregnancy (CP), and ongoing pregnancy (OP) rates were determined and compared between groups divided based on GM-CSF concentrations.ResultsThe detection rate of GM-CSF was found to be 50% in all SBCMs. There were significant differences in TQ rate, HP rate, CP rate and OP rate among high concentration group, medium concentration group and low concentration group. Both GM-CSF alone or GM-CSF combined with the morphological score (MS) had a greater AUC of ROC curve than that of MS alone to predict the pregnancy outcome, and GM-CSF combined with MS had the highest AUC.ConclusionsThe concentration of GM-CSF in SBCM was detected at fg/ml levels, which was associated with embryo quality and pregnancy outcome. Collectively, GM-CSF may be used as a biomarker for prediction of pregnancy outcome and selection of embryos with high developmental potential for transfer in assisted reproductive technology (ART).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Granulocyte-Macrophage Colony Stimulating Factor in Single Blastocyst Conditioned Medium as a Biomarker for Predicting Implantation Outcome of Embryo

et al. 2021

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“…A microarray analysis of the mid-secretory phase endometrium of patients suffering from recurrent implantation failure show a lower expression of IL-6, compared to healthy controls [ 28 ], further supporting the role of IL-6 in endometrial receptivity. Blastocysts also express and secrete IL-6 [ 29 , 30 , 31 , 32 ], and IL-6 in vitro treatment increases the embryos’ blastulation and hatching rates, compared to untreated embryos [ 33 ]. Furthermore, a higher IL-6 level in the follicular fluid also correlates with implantation success in patients undergoing in vitro fertilization (IVF) [ 34 ].…”

Section: Il-6 and Il-8 In A Healthy Pregnancymentioning

confidence: 99%

IL-6 and IL-8: An Overview of Their Roles in Healthy and Pathological Pregnancies

Vilotić

Nacka-Aleksić

Pirković

et al. 2022

IJMS

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Interleukin-6 (IL-6) is an acknowledged inflammatory cytokine with a pleiotropic action, mediating innate and adaptive immunity and multiple physiological processes, including protective and regenerative ones. IL-8 is a pro-inflammatory CXC chemokine with a primary function in attracting and activating neutrophils, but also implicated in a variety of other cellular processes. These two ILs are abundantly expressed at the feto-maternal interface over the course of a pregnancy and have been shown to participate in numerous pregnancy-related events. In this review, we summarize the literature data regarding their role in healthy and pathological pregnancies. The general information related to IL-6 and IL-8 functions is followed by an overview of their overall expression in cycling endometrium and at the feto-maternal interface. Further, we provide an overview of their involvement in pregnancy establishment and parturition. Finally, the implication of IL-6 and IL-8 in pregnancy-associated pathological conditions, such as pregnancy loss, preeclampsia, gestational diabetes mellitus and infection/inflammation is discussed.

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Interleukin-6 Concentration in Single-Embryo Medium Is Associated with Blastocyst Formation

Zha,

Yang,

Jiang

et al. 2023

Reprod. Sci.

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Detection of IL-6, IL-10, and TNF-α level in human single-blastocyst conditioned medium using ultrasensitive Single Molecule Array platform and its relationship with embryo quality and implantation: a pilot study

Cited by 5 publications

References 25 publications

Granulocyte-Macrophage Colony Stimulating Factor in Single Blastocyst Conditioned Medium as a Biomarker for Predicting Implantation Outcome of Embryo

Granulocyte-Macrophage Colony Stimulating Factor in Single Blastocyst Conditioned Medium as a Biomarker for Predicting Implantation Outcome of Embryo

IL-6 and IL-8: An Overview of Their Roles in Healthy and Pathological Pregnancies

Interleukin-6 Concentration in Single-Embryo Medium Is Associated with Blastocyst Formation

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