Detection of immune complexes. The use of radioimmunoassays with Clq and monoclonal rheumatoid factor.

Gabriel, Alexandre; Agnello, Vincent

doi:10.1172/jci108722

Cited by 139 publications

(44 citation statements)

References 26 publications

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“…Luthra et a1 (23) used a similar radioimmunoassay to detected immune complexes in 26% of sera and 22% of synovial fluids from patients with RA; Lindsley et a1 (6) found immune complexes in 32% of RA sera. Utilizing a radioassay based on competitive inhibition of the binding of radiolabeled MRF to IgGSepharose, Gabriel et a1 (20) It is of interest that the findings in this study suggested a correlation between C lq binding activity and some parameters of disease activity while the MRF-RIA and RC-RA correlated with extraarticular features. Zubler et a1 (7) have also noted a positive correlation between an elevated Clq binding and disease activity as measured by the joint score, sedimentation rate, and morning stiffness.…”

Section: Resultsmentioning

confidence: 61%

“…Synovial fluids from patients with joint counts of less than 10 showed lower levels of immune complexes than patients with joint counts greater than 10 (P = 0.015); sera from patients with morning stiffness of less than 2 hours gave lower values compared to those having a longer duration of morning stiffness (P = 0.03); sera from patients having sedimentation rates less than 50 mm/hr gave lower mean values of Clq binding than sera with sedimentation rates more compared to RA synovial fluid (18,19 (7) found immune complexes by Clq-BA in 76% of seropositive RA sera and in 80% of RA synovial fluids. Others have found a frequency of Clqreactive material in RA sera ranging from 48% to 87% (20)(21)(22).0nly a few reports are available concerning the detection of immune complex-like material in RA by MRF radioassays. Luthra et a1 (23) used a similar radioimmunoassay to detected immune complexes in 26% of sera and 22% of synovial fluids from patients with RA; Lindsley et a1 (6) found immune complexes in 32% of RA sera.…”

Section: Resultsmentioning

confidence: 99%

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Immune Complexes in Rheumatoid Arthritis Sera and Synovial Fluids

Halla

Volanakis

Schrohenloher

1979

Arthritis & Rheumatism

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Sera and synovial fluids from 88 patients with rheumatoid arthritis were examined for circulating immune complexes by three assays: monoclonal rheumatoid factor radioimmunoassay, Clq binding assay, and Raji cell radioassay. Paired samples were available for 82 patients. Immune complexes were detected with high frequency in the synovial fluid by each assay (75% by the monoclonal rheumatoid factor radioimmunoassay, 95% by the Clq binding assay, and 61% by the Raji cell radioassay). In rheumatoid arthritis sera, immune complexes were detected with high frequency by the Clq binding assay (85%) and the monoclonal rheumatoid factor radioimmunoassay (70%) but infrequently by the Raji cell radioassay (26%). The presence of immune complexes in serum was most frequently accompanied by the presence of complexes in fluid, regardless of the method of detection; moreover, the levels of immune complexes in synovial fluid were generally higher than in paired serum. Further, the levels of immune complexes as measured by the Clq binding assay correlated with certain parameters of clinical activity, while the monoclonal rheumatoid factor radioimmunoassay and Raji cell radioassay correlated with extraarticular features (excluding nodules) of rheumatoid arthritis. Available evidence suggests that immunologic processes initiate and/or sustain the inflammation of rheumatoid arthritis. This concept is based in part on the detection of immune complexes and complement abnormalities in both sera and synovial fluids from patients with this disease (1,2). Although numerous methods have been used for the detection of immune complexes, data comparing various procedures in rheumatoid arthritis are limited. The authors have previously presented (3) comparative data on the detection of immune complexes in sera and synovial fluids of rheumatoid arthritis patients by a radioimmunoassay based on reaction with monoclonal rheumatoid factor and gel diffusion procedures that use monoclonal rheumatoid factor and Clq. Immune complexes were detected at similar high frequencies in rheumatoid synovial fluids by precipitin methods and the radioimmunoassay; in contrast, immune complexes were detected with a much higher frequency in rheumatoid sera by the radioimmunoassay than by the precipitin methods. Levels of immune complexes noted by the radioimmunoassay in the sera were generally lower than those found in the synovial fluids. In addition, preliminary data from this and another laboratory have been obtained on the comparison of certain radioassays in various rheumatic diseases including rheumatoid arthritis (4-6). Different patterns of reactivity were observed for various diseases indicating differences in the biologic properties of the immune complexes detected.The frequency and levels of immune complexes by three radioassays (monoclonal rheumatoid factor radioimmunoassay, C 1 q binding assay, and Raji cell radioassay) in rheumatoid arthritis sera and synovial fluids are examined in the present study. Further, the

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Section: Resultsmentioning

confidence: 61%

Section: Resultsmentioning

confidence: 99%

Immune Complexes in Rheumatoid Arthritis Sera and Synovial Fluids

Halla

Volanakis

Schrohenloher

1979

Arthritis & Rheumatism

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show abstract

“…The lower molecular weight material that reacted mainly in the 1251-Clq binding assay may be similar to Clq-reactive, low molecular weight substances found in some patients with systemic lupus erythematosus (28). Its density and its failure to be detected in the Clq solid phase assay are incompatible with complement fixing immune complexes.…”

Section: Resultsmentioning

confidence: 85%

Circulating Immune Complexes in Lyme Arthritis

Hardin¹,

Walker²,

Steere³

et al. 1979

J. Clin. Invest.

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“…The Clq-inhibition (Clq-INH) and the mRF-inhibition (mRF-INH) assays were performed as described by Gabriel and Agnello (27). The assays quantitate the ability of immune complexes to inhibit the binding of radiolabeled Clq or mRF to normal human IgG coupled to a p-azobenzamidoethyl Sepharose-4B immunoadsorbent.…”

Section: Radioimmunoassaysmentioning

confidence: 99%