2010
DOI: 10.1002/elps.201000038
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Detection of immunogenic parasite and host‐specific proteins in the sera of active and chronic individuals infected with Toxoplasma gondii

Abstract: Toxoplasma gondii infection in pregnant women may result in abortion and foetal abnormalities, and may be life-threatening in immunocompromised hosts. To identify the potential infection markers of this disease, 2-DE and Western blot methods were employed to study the parasite circulating antigens and host-specific proteins in the sera of T. gondii-infected individuals. The comparisons were made between serum protein profiles of infected (n=31) and normal (n=10) subjects. Antigenic proteins were identified by … Show more

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Cited by 6 publications
(5 citation statements)
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“…In addition, five non-host factors were detected, including proteins from A. lwoffii, B. multivorans, M. xanthus, L. hongkogenisis , and S. salivarius . As previously suggested [88] , combined proteomic and serological approaches, such as the one used in the present study, can reflect numerous events occurring in vivo simultaneously due to the fact that patient serum is complex and consists of many proteins. Our data indicate that immunoproteomics approach could be a promising application for biomarker discovery and disease progression.…”
Section: Discussionmentioning
confidence: 95%
“…In addition, five non-host factors were detected, including proteins from A. lwoffii, B. multivorans, M. xanthus, L. hongkogenisis , and S. salivarius . As previously suggested [88] , combined proteomic and serological approaches, such as the one used in the present study, can reflect numerous events occurring in vivo simultaneously due to the fact that patient serum is complex and consists of many proteins. Our data indicate that immunoproteomics approach could be a promising application for biomarker discovery and disease progression.…”
Section: Discussionmentioning
confidence: 95%
“…Proteomic analysis by 2-DE has been widely used to identify specific bacterial or viral antigens during vaccine and/or serodiagnostic test development [ 23 ]. Nevertheless, there have been few proteomic studies focused at elucidating pathogenic mechanisms or identifying potential diagnostic markers in malaria [ 24 - 26 ].…”
Section: Discussionmentioning
confidence: 99%
“…In order to select proteins that present strong similarity with T. gondii sequences and in the same time the lowest similarity with any non-T. gondii sequences, we used a ToxoDB database search with MASCOT followed by a NCBI non-redundant protein database to deduce sequence homology of these 7 proteins against their closest relatives (Hammondia, Neospora, and Eimeria) and other organisms including humans [14,22].…”
Section: Discussionmentioning
confidence: 99%
“…Immunoproteomics combining one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (1D-SDS-PAGE) followed by immunoblot and nano-liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) analysis is a powerful tool for the identification of proteins with diagnostic value [6,[12][13][14].…”
Section: Introductionmentioning
confidence: 99%