Detection of immunoglobulin light‐chain restriction in cutaneous B‐cell lymphomas by ultrasensitive bright‐field <scp>mRNA</scp><i>in situ</i> hybridization

Minca, Eugen C.; Wang, Hongwei; Wang, Zhen; Lanigan, Christopher; Billings, Steven D.; Luo, Yuling; Tubbs, Raymond R.; Ma, Xiao‐Jun

doi:10.1111/cup.12415

Cited by 13 publications

(35 citation statements)

References 14 publications

(34 reference statements)

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“…Sixty-three relevant articles assessing kappa and lambda IHC or ISH in the evaluation of an atypical cutaneous or mucosal lymphoid infiltrate were identified ( Table 1, Table S1). 5,[9][10][11][12][13][14][15][16][17][18] 44 papers evaluated IHC exclusively, 10,11,13,15,17,[22][23][24][25][26][27][28][29]31,32,34,35,37,40,42,45,46,[48][49][50][52][53][54][55][57][58][59][60][61][62][63][64][65][66]…”

Section: Resultsmentioning

confidence: 99%

“…5,12,14,33,36,38,39,41,51,56 Two studies examined frozen tissue only, 58,65 five studies used frozen and FFPE tissue, 25,35,49,61,62 one study used formalin-or glutaraldehydefixed, paraffin-embedded tissue, 28 one study used formalin or B5-fixed, paraffin-embedded tissue, 50 one study used formalin or Zenker's fixed paraffin-embedded tissue, 71 one study used formalin and B5-fixed paraffin-embedded tissue and frozen tissue, 67 three studies did not specify fixation, 47,70,73 and the remainder utilized FFPE tissue. 5,[9][10][11][12][13][14][15][16][17][18][22][23][24]26,27,[29][30][31][32][33][34][36][37]…”

Section: Resultsmentioning

confidence: 99%

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Kappa and lambda immunohistochemistry and in situ hybridization in the evaluation of atypical cutaneous lymphoid infiltrates

et al. 2020

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Background: Atypical cutaneous lymphoid infiltrates are challenging lesions in dermatopathology. We present a summary of the literature regarding kappa and lambda immunohistochemistry (IHC) and in situ hybridization (ISH) in the evaluation of atypical cutaneous or mucosal lymphoid infiltrates. Methods: Relevant articles from 1967 to 2018 in the English language were identified and summarized. In the absence of larger studies, case series of n ≥ 3 were included. Results: Sixty-three articles assessing kappa and lambda IHC and/or ISH were identified. Most focused on marginal zone lymphomas. Other lymphomas included follicle center lymphoma, diffuse large B-cell lymphoma, chronic lymphocytic leukemia/small lymphocytic lymphoma, mantle cell lymphoma, lymphoplasmacytic lymphoma, plasmablastic lymphoma, multiple myeloma, monoclonal gammopathy of undetermined significance, and polyneuropathy, organomegaly, endocrinopathy, monoclonal protein, skin changes (POEMS). Non-neoplastic lesions included reactive lymphoid hyperplasia, cutaneous plasmacytosis, connective tissue disease, IgG4-related disease, acrodermatitis chronic atrophicans, Zoon balanitis, dermatitides, and infiltrates around epithelial dysplasias/neoplasias. Conclusion: Kappa and lambda IHC and ISH are useful tools in the evaluation of cutaneous B-cell lymphomas and plasma cell neoplasms. The literature supports that the detection of light-chain restriction by IHC and ISH is one of the most useful findings in the differential diagnosis of reactive lymphoid hyperplasia vs B-cell lymphoma with plasmacytic differentiation.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Kappa and lambda immunohistochemistry and in situ hybridization in the evaluation of atypical cutaneous lymphoid infiltrates

et al. 2020

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“…resolution in situ mRNA (RNA scope technique) also has been able to be used to demonstrate clonality. 212,213 Clusters of plasmacytoid dendritic cells are typically present. Another helpful immunophenotypic clue is the abundance of immunoglobulin G4 þ (IgG4 þ ) plasma cells found in a subset of such cases.…”

Section: Cutaneous B-cell Lymphomasmentioning

confidence: 99%

A Systematic Approach to the Cutaneous Lymphoid Infiltrates: A Clinical, Morphologic, and Immunophenotypic Evaluation

Gru

McHargue

Salavaggione

2019

Archives of Pathology &Amp; Laboratory Medicine

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Context.— The evaluation of cutaneous lymphoid infiltrates, both neoplastic and inflammatory, occurs very frequently in routine dermatopathologic examination and consultation practices. The “tough” cutaneous lymphoid infiltrate is feared by many pathologists; skin biopsies are relatively small, whereas diagnostic possibilities are relatively broad. It is true that cutaneous lymphomas can be difficult to diagnose and that in many circumstances multiple biopsies are required to establish a correct diagnostic interpretation. As a reminder, one should understand that low-grade cutaneous lymphomas are indolent disorders that usually linger for decades and that therapy does not result in disease cure. It is also important to remember that in most circumstances, those patients will die from another process that is completely unrelated to a diagnosis of skin lymphoma (even in the absence of specific therapy). Objective.— To use a clinicopathologic, immunophenotypic, and molecular approach in the evaluation of common lymphocytic infiltrates. Data Sources.— An in-depth analysis of updated literature in the field of cutaneous lymphomas was done, with particular emphasis on updated terminology from the most recent World Health Organization classification of skin and hematologic tumors. Conclusions.— A diagnosis of cutaneous lymphoid infiltrates can be adequately approached using a systematic scheme following the proposed ABCDE system. Overall, cutaneous T- and B-cell lymphomas are rare and “reactive” infiltrates are more common. Evaluation of lymphoid proliferations should start with a good sense of knowledge of the clinical presentation of the lesions, the clinical differential considerations, and a conscientious and appropriate use of immunohistochemistry and molecular tools.

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“…Minca et al [44] used ultrasensitive bright-field mRNA-ISH (BRISH) for in situ light-chain detection in cutaneous B cell lymphomas on 27 skin biopsies and excisions from patients with available results of B cell PCR-based clonality studies: 16 clonal B cell lesions and 11 non-clonal B cell proliferations. BRISH was successful in 15/16 clonal B cell lesions and 11/11 non-clonal proliferations.…”

Section: Ancillary Techniquesmentioning

confidence: 99%

New developments in the pathology of malignant lymphoma: a review of the literature published from October 2014–December 2014

Krieken

2015

J Hematopathol

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Detection of immunoglobulin light‐chain restriction in cutaneous B‐cell lymphomas by ultrasensitive bright‐field mRNAin situ hybridization

Abstract: Ultrasensitive BRISH can successfully detect light-chain restriction in B-NHL from FFPE skin specimens and may be a useful adjunct ancillary tool in cases not resolved by CISH or immunohistochemical methods.

Cited by 13 publications

References 14 publications

Kappa and lambda immunohistochemistry and in situ hybridization in the evaluation of atypical cutaneous lymphoid infiltrates

Kappa and lambda immunohistochemistry and in situ hybridization in the evaluation of atypical cutaneous lymphoid infiltrates

A Systematic Approach to the Cutaneous Lymphoid Infiltrates: A Clinical, Morphologic, and Immunophenotypic Evaluation

New developments in the pathology of malignant lymphoma: a review of the literature published from October 2014–December 2014

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