Detection of IncN‐pST15 one‐health plasmid harbouring bla _KPC‐2 in a hypermucoviscous Klebsiella pneumoniae CG258 isolated from an infected dog, Brazil

Abstract

The emergence and rapid spread of carbapenemase-producing Enterobacterales represents a serious public health concern. Critically, these global priority bacteria have begun to be reported in companion animals, implying a potential risk of crosstransmission between humans and pets. Using long-read (MinION) and short-read (Illumina) sequencing technologies, we have identified and characterized a hypermucoviscous KPC-2-producing Klebsiella pneumoniae strain belonging to the highrisk international clone ST11/CG258, in a dog with urinary tract infection. Strikingly, the bla KPC-2 gene was carried by a 54-kb IncN plasmid assignated to ST15, which shared 99.8 and 96.8% pairwise identity with IncN-pST15 plasmids from human and environmental K. pneumoniae strains, respectively; all come from an area with high endemicity of KPC-2. Our findings suggest that IncN-pST15 plasmids conferring carbapenem resistance can play as important a role as clonal transmission of K. pneumoniae, representing another major challenge for One Health.

Companion Animals—An Overlooked and Misdiagnosed Reservoir of Carbapenem Resistance

“…Briefly, three studies detected KPC-producing Escherichia coli and Klebsiella pneumoniae from dogs in Brazil and in Enterobacter xiangfangensis from a dog in the United States [9][10][11]. A IMP-4-enzyme in Salmonella isolates was recovered from a cat's faecal samples in Australia [12], VIM-2 in Pseudomonas aeruginosa from dogs with pyoderma and otitis in South Korea [13] and VIM-1 in K. pneumoniae from dogs in Spain [14].…”

Pandemic Clones of CTX-M-15 Producing Klebsiella pneumoniae ST15, ST147, and ST307 in Companion Parrots

“…This species is one of the most common MDR bacteria isolated in nosocomial and community-acquired infections in humans [ 24 ]. The reports about infection by K. pneumoniae in veterinary medicine have been associated with cats and dogs [ 7 , 25 , 26 , 27 , 28 ], and little is known about the impact of this pathogen in companion and wildlife bird species. In a previous study, colonization of passerine and psittacine seized from illegal trade in Brazil, by K. pneumoniae , was reported [ 9 ].…”

Characterization of 2 Klebsiella pneumoniae carbapenemase–producing Enterobacterales isolated from canine rectal swabs

“…19,26 KPC enzymes have been reported in clinical isolates from companion animals such as a dog in Brazil with a UTI caused by a K. pneumoniae harboring a bla KPC-2 gene, and 2 isolates of Enterobacter cloacae complex with bla KPC-4 in Ohio. 8,22 We report here 2 isolates of KPC-producing Enterobacterales identified at a veterinary teaching hospital (VTH) during the response to a CPE outbreak caused by a strain of E. coli harboring the bla NDM-5 gene. We performed whole-genome sequencing (WGS) to characterize the 2 isolates of KPC-producing Enterobacterales.…”

“…9 The main classes of carbapenemase enzymes are Klebsiella pneumoniae carbapenemase (KPC), New Delhi metallo-beta-lactamase (NDM), Verona integron-encoded metallo-beta-lactamase (VIM), imipenemase (IMP), and OXA-48/Oxa-48-like. 9 Reports of CPE have increased in companion animal veterinary medicine 7,8,18,21,22 ; descriptions of CPE outbreaks in veterinary settings demonstrate the importance of surveillance and isolate characterization. 7,21 Klebsiella pneumoniae carbapenemase (KPC) enzymes, the first of which (KPC-1) was reported in 2001 in North Carolina, are Ambler class A beta-lactamases (serine beta lactamase), and at the time of discovery were unique from previously described carbapenemase enzymes (class B or metallo-beta-lactamases).…”

“…9 The main classes of carbapenemase enzymes are Klebsiella pneumoniae carbapenemase (KPC), New Delhi metallo-beta-lactamase (NDM), Verona integron-encoded metallo-beta-lactamase (VIM), imipenemase (IMP), and OXA-48/Oxa-48–like. 9 Reports of CPE have increased in companion animal veterinary medicine 7,8,18,21,22 ; descriptions of CPE outbreaks in veterinary settings demonstrate the importance of surveillance and isolate characterization. 7,21…”