Detection of inducible clindamycin resistance among Staphylococcal isolates from different clinical specimens in western India

N, Pal; Sharma, Babita; Sharma, Rameshwar K.; Vyas, Leela

doi:10.4103/0022-3859.68637

Cited by 38 publications

(38 citation statements)

References 17 publications

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“…Both inducible and constitutive MLS B phenotypes, as well as MS phenotypes, were reported to occur, but with slightly different frequencies (21). Among the erythromycinresistant staphylococcal isolates of the present study, 30.5% expressed the MLS B phenotype constitutively and 19.1% inducibly.…”

Section: Discussionmentioning

confidence: 74%

Macrolide and lincosamide resistance in staphylococcal clinical isolates in Nablus, Palestine

Al-Masri¹,

Hasan²,

Sabbah³

2016

Turk J Med Sci

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Background/aim: Macrolide and lincosamide antibiotics are used for the treatment of staphylococcal infections, especially for penicillin-allergic patients. In the present study, we evaluate the prevalence of resistance to macrolide and lincosamide antibiotics among staphylococci isolates. Materials and methods:A total of 200 staphylococcal clinical isolates were collected from January 2012 to April 2013. Minimal inhibitory concentrations of erythromycin and clindamycin were determined by agar dilution method. An erythromycin-clindamycin induction test was performed for isolates that were only resistant to erythromycin. Representative erythromycin-resistant isolates were examined for erythromycin resistance genes using PCR.Results: Among staphylococci isolates, resistance frequencies of erythromycin and clindamycin were 65.5% and 20.5%, respectively. Erythromycin resistance was found to be mediated by putative efflux (50.4%) and target site modification (49.6%). Inducible target site modification resistance was detected in 19.1% of erythromycin-resistant isolates. Among the examined 36 staphylococci isolates, msr(A), erm(C), erm(A), and mef(A/E) genes were detected in 55.6%, 30.6%, 25%, and 0%, respectively. Conclusion:Results of the current study indicate the presence of high rates of macrolide resistance and inducible phenotypes among staphylococcal isolates. It is also essential to keep in mind variations of resistance rates among various age groups and specimen types.

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Section: Discussionmentioning

confidence: 74%

Macrolide and lincosamide resistance in staphylococcal clinical isolates in Nablus, Palestine

Al-Masri¹,

Hasan²,

Sabbah³

2016

Turk J Med Sci

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“…Amerika Birleşik Devletleri'nde yapıl-mış iki ayrı çalışmada, S.aureus ve KNS suşlarında iMLS B direnç oranı %30 civarında iken, diğer bir çalışmada %20 civarında olmak üzere birbirinden farklı oranlar bildirilmiştir 16,17 . Hindistan'da yapılan bir çalışmada, suşların %24'ünde iMLS B , %47'sinde cMLS B direnci gözlenmiştir 18 . Bizim çalışmamızda, indüklenebilir tipte direnç kromozomal dirençten daha sık saptanmış; iMLS B direnci ise MRSA'da %22, MSSA'da %4, MRKNS'lerde %49 ve MSKNS'lerde %21 olarak bulunmuştur (Tablo I).…”

Section: Discussionunclassified

Macrolide-Lincosamide-Streptogramin B (MLSB) Resistance Phenotypes in Clinical Staphylococcus Isolates and Investigation of Telithromycin Activity

Uzun¹,

Güngör²,

Pektaş³

et al. 2014

Mikrobiyol Bul

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“…In two other studies, the sensitivity for inducible clindamycin resistance detection was 99% (Griffith et al, 2007;Sharp et al, 2009). In a study (Pal et al, 2010) sensitivity and specificity of vitek-2 system is 93 and 100%, respectively. In the present study, the Vitek 2 card failed to detect inducible clindamycin resistance in 2 strains (negative predictive value of 96.6%.…”

Section: Discussionmentioning

confidence: 99%

“…The MS and iMLS B phenotypes are indistinguishable by using standard susceptibility test methods, but can be distinguished by erythromycin-clindamycin disk approximation test (Dtest), automated vitek-2 system and demonstration of resistance genes by molecular methods (Steward et al, 2005;Pal et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Detection of inducible clindamycin resistance by an automated system in a tertiary care hospital

Urmi¹,

Summaiya²,

Latika³

et al. 2011

Afr. J. Microbiol. Res.

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Clindamycin is commonly used in treatment of erythromycin resistant Staphylococcus aureus causing skin and soft tissue infections. In vitro routine tests for clindamycin susceptibility may fail to detect inducible clindamycin resistance due to 'erm' genes resulting in treatment failure thus necessitating the need to detect such resistance by a rapid method. In the era of automation, vitek-2 system provides a panel for detection of inducible clindamycin resistance with conjunction of other antimicrobial susceptibility testing. The present study evaluated the performance of a vitek-2 card for detection of inducible clindamycin resistance in a tertiary care hospital. Non-duplicate clinical isolates of hundred S. aureus were obtained from various clinical samples. Antimicrobial susceptibility testing was carried out, including detection of clindamycin resistance and methicillin resistance pattern by vitek-2 identification and antimicrobial susceptibility testing (ID/AST) system by using AST-GP67 card. The results were compared to those of D-test as per CLSI guidelines on erythromycin resistant isolates. EPI INFO software, Licensed by CDC Atlanta was used for analysis of data. The sensitivity and specificity for the vitek-2 card was 95.4 and 100%, respectively in comparism to disk approximation test (D-test),. The performance of vitek-2 card was 100% specific and rapid for detection of inducible clindamycin resistance with other antimicrobial susceptibility results.

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Detection of inducible clindamycin resistance among Staphylococcal isolates from different clinical specimens in western India

Cited by 38 publications

References 17 publications

Macrolide and lincosamide resistance in staphylococcal clinical isolates in Nablus, Palestine

Macrolide and lincosamide resistance in staphylococcal clinical isolates in Nablus, Palestine

Macrolide-Lincosamide-Streptogramin B (MLSB) Resistance Phenotypes in Clinical Staphylococcus Isolates and Investigation of Telithromycin Activity

Detection of inducible clindamycin resistance by an automated system in a tertiary care hospital

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