Detection of infectious bronchitis virus serotypes by reverse transcription polymerase chain reaction in broiler chickens

Jahantigh, Mohammad; Salari, Saeed; Hedayati, Mehdi

doi:10.1186/2193-1801-2-36

Cited by 17 publications

(18 citation statements)

References 10 publications

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“…The RNA was stored at −20°C until use. Amplification RT-PCR was performed with the following primer pairs, as described in Table-1: UTR41+/11− targeting the 3' untranslated region (3'-UTR) of the avian coronavirus genome [13], IBVN+/IBVN− targeting the N gene of IB [14], and XCE2+/XCE2− targeting the S1 gene [15]. Polymerase chain reaction amplification was carried out using the Bioline One-…”

Section: Viral Rna Extractionmentioning

confidence: 99%

“…Step RT-PCR kit (Bioline, UK) with the following thermal cycling profile: Reverse transcription at 45°C for 20 min, polymerase activation at 95°C for 1 min, then 40 cycles of denaturation at 95°C for 15 s, annealing temperatures and times specific for each primer pair and described below, and extension at 72°C for 30 s. This was followed by a final extension at 72°C for 7 min. Annealing occurred at 48°C for 1 min, 58°C for 30 s, and 50°C for 30 s for UTR41+/11− [13], the partial N gene [14], and the partial S1 gene [15], respectively.…”

Section: Viral Rna Extractionmentioning

confidence: 99%

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Isolation and characterization of avian coronavirus from healthy Eclectus parrots (Eclectus roratus) from Indonesia

et al. 2019

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Background and Aim: Avian coronavirus has a wide range of hosts, from chickens and turkeys to wild birds. This virus causes an economically and, possibly, environmentally, important loss in the poultry industry. Therefore, research into the avian coronavirus in various species of birds is required. The Eclectus parrot (Eclectus roratus) is an endemic bird to Indonesia and Northern Australia and often kept as pets. At present, there has been limited information about avian coronavirus infection among birds. This study aimed to determine the presence of and to characterize avian coronavirus isolated from Eclectus parrots in Indonesia. Materials and Methods:Cloacal swab samples were taken from 10 healthy Eclectus parrots (E. roratus). Each isolate was propagated into specific pathogen-free embryonated chicken eggs. The presence of avian coronavirus was determined using three sets of primers targeting the 3' untranslated region (3'-UTR) of avian coronavirus (UTR41+/11−), the N gene of the infectious bronchitis virus (IBVN+/−), and the S1 gene of the IBV (XCE2+/XCE2−). The infectious bronchitis vaccine strain H120 was used as a positive control. Resulting positive bands were sequenced for the S1 gene.Results: None of the isolates was positive for the 3'-UTR, four isolates were positive for the N gene of infectious bronchitis, and two isolates were positive for the S1 gene of the IBV. However, only one isolate (parrot/Indonesia/BX9/16) was sequenced for the partial S1 gene with primers XCE2+/XCE2−. The partial nucleotide sequence of this isolate showed 100% homology with the IBV GI-13 lineage, specifically with a field isolate of the 4/91 variant 1 Israel and the 4/91 vaccine on the hypervariable region 3 site of the S1 gene. Conclusion:An IB-like avian coronavirus was isolated from healthy Eclectus parrots. Our results indicate that IBV has a wide range of hosts, which prompt the need to understand the interspecies connection of this virus better.

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Section: Viral Rna Extractionmentioning

confidence: 99%

Section: Viral Rna Extractionmentioning

confidence: 99%

Isolation and characterization of avian coronavirus from healthy Eclectus parrots (Eclectus roratus) from Indonesia

et al. 2019

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“…The virus replicates in epithelial cells of the upper respiratory tract, producing different respiratory troubles, noisy respirations, and the formation of caseated plugs in tracheal bifurcation. Other IBV serotypes replicate mainly in epithelial cells of the kidney tubules or oviduct causing nephritis and decreased egg production [7][8][9][10]. Avian influenza virus (AIV) is members of the family Orthomyxoviridae.…”

Section: Introductionmentioning

confidence: 99%

“…Different studies showed that many organisms such as S. aureus, Haemophilus paragallinarum, E. coli, Ornithobacterium rhinotracheale, MG, MS, IBV, NDV, and even live IBV and NDV vaccines have synergistic effects that enhance the virulence of H9N2 and increase mortality in infected birds [7,[18][19][20][21][22][23]. Such synergistic effects may be occurring through enhancing hyaluronic acid (HA) cleavage by secretion of trypsin-like proteases [18,24,25].…”

Section: Introductionmentioning

confidence: 99%

Molecular survey and interaction of common respiratory pathogens in chicken flocks (field perspective)

Abdelaziz

Mohamed

Fayez³

et al. 2019

Vet World

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Aim:The present study was designed for the detection of the most prevalent respiratory infections in chicken flocks and clarifying their interaction and impact on flock health. Materials and Methods:A total of 359 serum samples were collected from 55 backyard chickens and tested using commercial enzyme-linked immunosorbent assay kits to determine the seroprevalence of Newcastle disease virus (NDV), infectious bronchitis virus (IBV), influenza type A, Mycoplasma gallisepticum (MG), and Mycoplasma synoviae (MS). Molecular prevalence of NDV, IBV, low pathogenic avian influenza virus (LPAIV) H9N2, MG, and MS was carried out on swab, and tissue samples collected from 55 backyard flocks and 11 commercial broiler flocks suffered from respiratory infections using polymerase chain reaction (PCR) and reverse transcription-PCR.Results: Seroprevalence of NDV, IBV, Influenza type A virus, MG, and MS in chicken backyard flocks was 56.4%, 50.9%, 12.7%, 14.5%, and 3.6%, respectively. Specific antibodies against one or more respiratory viruses and mycoplasma were detected in 36.4% of backyard flocks, indicating concurrent viral infections. The molecular survey showed that 90.9% of chicken backyard flocks were infected with common respiratory viruses (NDV, IBV, and LPAIV H9N2) while 81.8% of commercial broiler flocks were infected. The molecular prevalence rate of NDV, IBV, and LPAIV H9N2 was 46.97%, 56.1%, and 19.7% in backyard flocks, respectively. Combined viral and bacterial infection represented 40% and 63.6% of the respiratory infections, resulting in enhanced pathogenicity and increased mortalities of up to 87.5% and 27.8% in backyard and commercial flocks, respectively. Mixed infection of IBV, LPAIV H9N2, and/or Escherichia coli is the most prevalent mixed infection in broiler flocks, inducing severe clinical outcomes. Avian pathogenic E. coli was, respectively, isolated from 40% of backyard flocks and 81.82% of broiler flocks. Staphylococcus aureus was isolated from three backyard chicken flocks mixed with other respiratory pathogens with elevated mortality. Mixed infection of E. coli and MG reported in 9.1% of broiler flock. MG was detected in 14.5% of backyard flocks and 9.1% of broiler flocks while MS was detected only in 3.6% of backyard chickens mixed with E. coli, and other viruses. Conclusion:Our results confirm that mixed infections are more commonly prevalent and associated with dramatic exacerbation in clinical outcomes than a single infection. Bidirectional synergistic interaction between these concurrently interacted respiratory pathogens explains the severe clinical impact and high mortality rate. The high prevalence of IBV (either as a single or combined infection) with LPAIV H9N2 and/or E. coli, in spite of intensive use of commercial vaccines, increases the need for revising vaccination programs and the application of standard biosecurity measures. Backyard chickens impose a great risk and threaten commercial flocks due to the high prevalence of viral respiratory pathogens.

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“…Evolution of new IBV variants is an ongoing process which is associated with frequent point mutations in the nucleotide sequences of the S1 subunit mainly without alteration of the remaining viral genome (Jackwood, 2012). This genetic variability represents a modified method of the virus to immune selective pressures due to the extensive use of IBV vaccines allowing new field strains to evolve (Jahantigh et al, 2013). Although many…”

Section: Introductionmentioning

confidence: 99%

Molecular and Histopathological Investigation of Avian Infectious Bronchitis Virus in the Delta of Egypt between 2016 and 2017

Magouz

Abdo

Abdelsabour

et al. 2018

PVJ

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Infectious bronchitis virus (IBV) is a highly contagious viral disease of Family Coronaviridae. The current study reports the molecular and histopathological investigation of IBV strains emerged in outbreaks in the Nile Delta of Egypt. Tissue specimens from thirty-six chicken flocks were subjected to virus isolation, histopathology and PCR. Twenty-three isolates were confirmed as IBVs based on the amplification of the highly conserved nucleocapsid (N) gene. Further characterization of five selected isolates was done by amplification of the (S1) glycoprotein gene. Phylogenetic analysis revealed that the isolated strains were clustered into three distinct groups within the variant II clade. Alignment of S1gene amino acid sequences showed significant amino acid substitutions in relation to the currently used vaccinal strains which displays that new IBV variants which are evolutionarily distant from vaccinal strains are still emerging within the Egyptian chicken flocks and are mostly associated with acute tubular nephrosis syndrome.

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Detection of infectious bronchitis virus serotypes by reverse transcription polymerase chain reaction in broiler chickens

Cited by 17 publications

References 10 publications

Isolation and characterization of avian coronavirus from healthy Eclectus parrots (Eclectus roratus) from Indonesia

Isolation and characterization of avian coronavirus from healthy Eclectus parrots (Eclectus roratus) from Indonesia

Molecular survey and interaction of common respiratory pathogens in chicken flocks (field perspective)

Molecular and Histopathological Investigation of Avian Infectious Bronchitis Virus in the Delta of Egypt between 2016 and 2017

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