Detection of infectious bronchitis virus

Wit, Janneke

doi:10.1080/03079450094108

Cited by 167 publications

(142 citation statements)

References 163 publications

(328 reference statements)

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“…For example, the turkey coronaviruses replicate to about 10 4 -fold higher titres in turkey embryos than in chick embryos (Adams & Hofstad, 1971). The chick-embryo titres of the two viruses isolated in this study were similar to those of IBV strains (De Wit, 2000), as were the effects that virus replication had on embryo development. From the third to the fifth passage, the coronaviruses (as confirmed by EM) from the teal and peafowl induced embryo dwarfing, stunting, curling and death, characteristics that are typical of 'field' IBV strains (Clarke et al, 1972;De Wit, 2000).…”

Section: Discussionsupporting

confidence: 52%

Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas)

Liu

Chen

et al. 2005

Journal of General Virology

132

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Coronavirus-like viruses, designated peafowl/China/LKQ3/2003 (pf/CH/LKQ3/03) and teal/China/LDT3/2003 (tl/CH/LDT3/03), were isolated from a peafowl and a teal during virological surveillance in Guangdong province, China. Partial genomic sequence analysis showed that these isolates had the S-3-M-5-N gene order that is typical of avian coronaviruses. The spike, membrane and nucleocapsid protein genes of pf/CH/LKQ3/03 had >99 % identity to those of the avian infectious bronchitis coronavirus H120 vaccine strain (Massachusetts serotype) and other Massachusetts serotype isolates. Furthermore, when pf/CH/LKQ3/03 was inoculated experimentally into chickens (specific-pathogen-free), no disease signs were apparent. tl/CH/LDT3/03 had a spike protein gene with 95 % identity to that of a Chinese infectious bronchitis virus (IBV) isolate, although more extensive sequencing revealed the possibility that this strain may have undergone recombination. When inoculated into chickens, tl/CH/LDT3/03 resulted in the death of birds from nephritis. Taken together, this information suggests that pf/CH/LKQ3/03 might be a revertant, attenuated vaccine IBV strain, whereas tl/CH/LDT3/03 is a nephropathogenic field IBV strain, generated through recombination. The replication and non-pathogenic nature of IBV in domestic peafowl and teal under field conditions raises questions as to the role of these hosts as carriers of IBV and the potential that they may have to transmit virus to susceptible chicken populations. INTRODUCTIONCoronaviruses (family Coronaviridae) belong to the order Nidovirales and contain a positive-stranded RNA genome that ranges from 27 to 31 kb in size (Cavanagh, 1997). Members of the family Coronaviridae infect a wide range of hosts and have been classified into three groups on the basis of antigenicity, genome organization and sequence similarity. Usually, coronaviruses infect only their normal target host species. It has, however, been reported that some strains of canine coronavirus and human coronavirus 229E can infect other, non-target species without causing disease (Barlough et al., 1984(Barlough et al., , 1985. The recent emergence of severe acute respiratory syndrome coronavirus (SARSCoV), which has been classified tentatively into group 2, has focused a great deal of interest on this virus family (Holmes, 2003). It was reported that SARS-CoV-like viruses were isolated from Himalayan palm civets (Guan et al., 2003) and ferrets (Mustela furo). Moreover, domestic cats (Felis domesticus) are susceptible to infection by SARSCoV, suggesting that the reservoir for this pathogen might involve a range of animal species (Martina et al., 2003).Infectious bronchitis virus (IBV), together with genetically related coronaviruses of turkey and pheasant, belongs to the group 3 coronaviruses. IBV is a pleomorphic, enveloped virus with club-shaped surface projections (spikes) and a single-stranded, positive-sense RNA genome of >27 kb in length (Boursnell et al., 1987). Upon virus entry into cells, a 39-coterminal nested set of six ...

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Section: Discussionsupporting

confidence: 52%

Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas)

Liu

Chen

et al. 2005

Journal of General Virology

132

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“…As previously discussed in the case of infectious bronchitis (IB) (De Wit, 2000), the selection of the most appropriate test and subsequent interpretation of the results is not a simple matter, because of the lack of scientific data on the performance of individual tests. The situation is further complicated in the case of APV.…”

Section: Introductionmentioning

confidence: 99%

Detection and differentiation of avian pneumoviruses (metapneumoviruses)

Cook¹,

Cavanagh

2002

Avian Pathology

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The available detection methods for avian pneumoviruses (turkey rhinotracheitis virus; genus Metapneumovirus) in turkeys, domestic fowl and other species are reviewed. The advantages and disadvantages of virus isolation techniques, virus or genome (polymerase chain reaction) detection and serology are discussed. Some of the problems likely to be encountered are considered, including the detection of yet to be discovered subtypes, as are the factors that are likely to influence the outcome of the work.

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“…Portanto, a utilização de anticorpos monoclonais contra essa proteína viral é de grande valor para a detecção direta do VBI em amostras teciduais suspeitas de infecção, por meio de técnicas de imunofluorescência, imunoperoxidase ou de ELISA (DE WIT, 2000;NDIFUNA, et al, 1998).…”

Section: Resultsunclassified

“…Há ainda algumas técnicas como a reação de imunoflorescência e ELISA, em que o uso de anticorpos monoclonais para antígenos específicos desse vírus, como a nucleoproteína N, facilita e garante a rapidez na detecção desse patógeno viral em amostras teciduais de aves suspeitas de infecção (DE WIT, 2000).…”

Section: Anticorpos Monoclonais Constituem a Base De Vários Testes Usunclassified

Construção de uma biblioteca de fragmentos de anticorpos monoclonais de galinhas com cadeia única (scFv) por phage display com reatividade cruzada para estirpes heterólogas do vírus da bronquite infecciosa aviária

et al. 2010

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Detection of infectious bronchitis virus

Cited by 167 publications

References 163 publications

Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas)

Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas)

Detection and differentiation of avian pneumoviruses (metapneumoviruses)

Construção de uma biblioteca de fragmentos de anticorpos monoclonais de galinhas com cadeia única (scFv) por phage display com reatividade cruzada para estirpes heterólogas do vírus da bronquite infecciosa aviária

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